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Literature summary for 6.3.1.2 extracted from

  • Chen, J.; Kennedy, I.R.
    Purification and properties of lupin nodule glutamine synthetase (1985), Phytochemistry, 24, 2167-2172.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
cysteine
-
Lupinus luteus

General Stability

General Stability Organism
keto compounds, e.g. 2-oxoglutarate, oxaloacetate or pyruvate, enhance stability during purification Lupinus luteus
mercaptoethanol and divalent metal ions are essential to maintain the enzyme activity Lupinus luteus

Inhibitors

Inhibitors Comment Organism Structure
Ala
-
Lupinus luteus
Asp
-
Lupinus luteus
Ca2+ strong inhibition of Mg2+-activated enzyme Lupinus luteus
Co2+ strong inhibition of Mg2+-activated enzyme Lupinus luteus
Gly
-
Lupinus luteus
Mn2+ strong inhibition of Mg2+-activated enzyme Lupinus luteus
additional information combined inhibition by Gly, Ala and Ser is cumulative Lupinus luteus
phosphate transferase assay an biosynthetic assay Lupinus luteus
Ser
-
Lupinus luteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000597
-
ADP gamma-glutamyl transferase assay Lupinus luteus
0.0125
-
NH4+ biosynthetic assay Lupinus luteus
0.22
-
ATP biosynthetic assay Lupinus luteus
3.37
-
hydroxylamine gamma-glutamyl transferase assay Lupinus luteus
8.58
-
Glu biosynthetic assay Lupinus luteus
48.6
-
Gln gamma-glutamyl transferase assay Lupinus luteus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Lupinus luteus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal ratio of Mg2+ to ATP is 3:1 Lupinus luteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41500
-
x * 41500, SDS-PAGE Lupinus luteus
347000
-
gel filtration Lupinus luteus

Organism

Organism UniProt Comment Textmining
Lupinus luteus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lupinus luteus

Source Tissue

Source Tissue Comment Organism Textmining
root nodule
-
Lupinus luteus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
62
-
gamma-glutamylhydroxamate assay Lupinus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + NH4+
-
Lupinus luteus ADP + phosphate + L-Gln
-
?
Gln + hydroxylamine + ADP
-
Lupinus luteus gamma-Glutamylhydroxamate + NH4+ + ?
-
?

Subunits

Subunits Comment Organism
? x * 41500, SDS-PAGE Lupinus luteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
translase assay Lupinus luteus
7.9
-
biosynthetic assay Lupinus luteus