Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | - |
Lupinus luteus |
General Stability | Organism |
---|---|
keto compounds, e.g. 2-oxoglutarate, oxaloacetate or pyruvate, enhance stability during purification | Lupinus luteus |
mercaptoethanol and divalent metal ions are essential to maintain the enzyme activity | Lupinus luteus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ala | - |
Lupinus luteus | |
Asp | - |
Lupinus luteus | |
Ca2+ | strong inhibition of Mg2+-activated enzyme | Lupinus luteus | |
Co2+ | strong inhibition of Mg2+-activated enzyme | Lupinus luteus | |
Gly | - |
Lupinus luteus | |
Mn2+ | strong inhibition of Mg2+-activated enzyme | Lupinus luteus | |
additional information | combined inhibition by Gly, Ala and Ser is cumulative | Lupinus luteus | |
phosphate | transferase assay an biosynthetic assay | Lupinus luteus | |
Ser | - |
Lupinus luteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000597 | - |
ADP | gamma-glutamyl transferase assay | Lupinus luteus | |
0.0125 | - |
NH4+ | biosynthetic assay | Lupinus luteus | |
0.22 | - |
ATP | biosynthetic assay | Lupinus luteus | |
3.37 | - |
hydroxylamine | gamma-glutamyl transferase assay | Lupinus luteus | |
8.58 | - |
Glu | biosynthetic assay | Lupinus luteus | |
48.6 | - |
Gln | gamma-glutamyl transferase assay | Lupinus luteus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Lupinus luteus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimal ratio of Mg2+ to ATP is 3:1 | Lupinus luteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41500 | - |
x * 41500, SDS-PAGE | Lupinus luteus |
347000 | - |
gel filtration | Lupinus luteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lupinus luteus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lupinus luteus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root nodule | - |
Lupinus luteus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
62 | - |
gamma-glutamylhydroxamate assay | Lupinus luteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + NH4+ | - |
Lupinus luteus | ADP + phosphate + L-Gln | - |
? | |
Gln + hydroxylamine + ADP | - |
Lupinus luteus | gamma-Glutamylhydroxamate + NH4+ + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41500, SDS-PAGE | Lupinus luteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
translase assay | Lupinus luteus |
7.9 | - |
biosynthetic assay | Lupinus luteus |