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Literature summary for 6.3.1.2 extracted from
- Purification and properties of glutamine synthetase from spinach leaves (1985), Plant Physiol., 79, 923-927.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Met | irreversible, competitive | Spinacia oleracea |  |
| Phosphinothricin | irreversible, competitive | Spinacia oleracea | |
| Sulfoxamine | irreversible, competitive | Spinacia oleracea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
ATP | synthetase assay | Spinacia oleracea | |
| 6.7 | - |
Glu | synthetase assay | Spinacia oleracea | |
| 37.6 | - |
Gln | transferase assay | Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
8 * 44000, enzyme form GS2, SDS-PAGE | Spinacia oleracea |
| 360000 | - |
native PAGE | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 198 | - |
- |
Spinacia oleracea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for several days | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Glu + NH4+ | - |
Spinacia oleracea | ADP + phosphate + L-Gln | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 44000, enzyme form GS2, SDS-PAGE | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
transferase activity in presence of Mn2+ | Spinacia oleracea |
| 7.3 | - |
in presence of Mg2+ | Spinacia oleracea |
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Release 2023.1 (January 2023)
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