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Literature summary for 6.3.1.17 extracted from

  • Collard, F.; Stroobant, V.; Lamosa, P.; Kapanda, C.N.; Lambert, D.M.; Muccioli, G.G.; Poupaert, J.H.; Opperdoes, F.; van Schaftingen, E.
    Molecular identification of N-acetylaspartylglutamate synthase and beta-citrylglutamate synthase (2010), J. Biol. Chem., 285, 29826-29833.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.1.17

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol stimulates Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in bacteria or HEK293T cells Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0096
-
 ATP pH 8.0, 30°C Mus musculus
0.73
-
 L-glutamate pH 8.0, 30°C Mus musculus
1.24
-
 citrate pH 8.0, 30°C Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 x * 42000, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + citrate + L-glutamate Mus musculus
-
 ADP + phosphate + beta-citryl-L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q80WS1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
central nervous system
-
 Mus musculus
-
placenta
-
 Mus musculus
-
testis
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7
-
 pH 8.0, 30°C, formation of beta-citryl-L-glutamate Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + citrate + L-glutamate
-
 Mus musculus ADP + phosphate + beta-citryl-L-glutamate
-
 ?
ATP + citrate + L-glutamate the enzyme also has the activity of EC 6.3.2.41, N-acetylaspartylglutamate synthase. It catalyses the synthesis of beta-citryl-L-glutamate and N-acetyl-L-aspartyl-L-glutamate at nearly equal rates Mus musculus ADP + phosphate + beta-citryl-L-glutamate
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
NAAG synthetase I
-
 Mus musculus
NAAGS-I
-
 Mus musculus
RIMKLB gene name, ambiguous Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
 L-glutamate pH 8.0, 30°C Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Mus musculus