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Literature summary for 6.3.1.13 extracted from

  • Vargas, D.; Hageman, S.; Gulati, M.; Nobile, C.J.; Rawat, M.
    S-nitrosomycothiol reductase and mycothiol are required for survival under aldehyde stress and biofilm formation in Mycobacterium smegmatis (2016), IUBMB Life, 68, 621-628 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.1.13

Cloned(Commentary)

Cloned (Comment) Organism
gene mshC, enzyme expression analysis Mycolicibacterium smegmatis

Protein Variants

Protein Variants Comment Organism
additional information generation of mshC transposon disruption mutants, with or without co-disruption of gene mscR Mycolicibacterium smegmatis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP Mycolicibacterium smegmatis
-
 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate
-
 ?
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP Mycolicibacterium smegmatis mc2155
-
 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis A0QZY0
-
-
Mycolicibacterium smegmatis mc2155 A0QZY0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP
-
 Mycolicibacterium smegmatis 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate
-
 ?
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP
-
 Mycolicibacterium smegmatis mc2155 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
MSH ligase
-
 Mycolicibacterium smegmatis
MshC
-
 Mycolicibacterium smegmatis
mycothiol ligase
-
 Mycolicibacterium smegmatis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mycolicibacterium smegmatis

Expression

Organism Comment Expression
Mycolicibacterium smegmatis gene mshC is induced by S-nitrosoglutathione (GSNO) and aldehydes. Gene mshC expression increases under aldehyde stress and nitrosative stress up

General Information

General Information Comment Organism
malfunction Mycobacterium smegmatis mutants disrupted in mscR, coding for a dual function S-nitrosomycothiol reductase and formaldehyde dehydrogenase, and mshC, coding for a mycothiol ligase, EC 6.3.1.13, and lacking mycothiol (MSH), are more susceptible to S-nitrosoglutathione (GSNO) and aldehydes than wild-type. MSH is a cofactor for MscR, and both mshC and mscR are induced by GSNO and aldehydes. The transposon mutant, S24, disrupted in mshC, is most sensitive to killing by GSNO Mycolicibacterium smegmatis
metabolism the enzyme catalyzes the fourth step of a five step mycothiol, MSH, biosynthetic pathway Mycolicibacterium smegmatis
physiological function mycothiol and S-nitrosomycothiol reductase are required for normal biofilm formation in Mycobacterium smegmatis Mycolicibacterium smegmatis