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Literature summary for 6.3.1.1 extracted from

  • Meister, A.
    Asparagine synthesis (1974), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 10, 561-580.
No PubMed abstract available

General Stability

General Stability Organism
glycerol and 2-mercaptoethanol stabilize Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
Escherichia coli
L-Asp
-
Escherichia coli
L-Asp
-
Streptococcus equinus
N-ethylmaleimide
-
Escherichia coli
p-mercuribenzoate
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
ATP
-
Lactiplantibacillus plantarum
4
-
NH4+ ATP Streptococcus equinus
4.2
-
L-Asp
-
Lactiplantibacillus plantarum
26
-
L-Asp
-
Streptococcus equinus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Lactiplantibacillus plantarum
Mg2+ required Escherichia coli
Mg2+ required Streptococcus equinus
Mg2+ Km: 45 mM Streptococcus equinus
Mn2+ activates Lactiplantibacillus plantarum
Mn2+ Km: 3.5 mM Lactiplantibacillus plantarum
Mn2+ no activation Escherichia coli
Mn2+ no activation Streptococcus equinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K-12
-
Escherichia coli
-
overexpression of the fusion protein constructed of asparagine synthetase A structural gene is fused to the 3´end of the human carbonic anhydrase II structural gene
-
Lactiplantibacillus plantarum
-
-
-
Streptococcus equinus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Asp + hydroxylamine
-
Escherichia coli AMP + diphosphate + Asn + beta-aspartyl hydroxamate
-
?
ATP + L-Asp + hydroxylamine hydroxylamine is more active than NH4+ Lactiplantibacillus plantarum AMP + diphosphate + Asn + beta-aspartyl hydroxamate
-
?
ATP + L-Asp + hydroxylamine hydroxylamine is more active than NH4+ Streptococcus equinus AMP + diphosphate + Asn + beta-aspartyl hydroxamate
-
?
ATP + L-Asp + NH4+
-
Streptococcus equinus AMP + diphosphate + Asn
-
?
ATP + L-Asp + NH4+ no activity with GTP Lactiplantibacillus plantarum AMP + diphosphate + Asn
-
?
ATP + L-Asp + NH4+ no activity with ADP, CTP, UTP, or GTP Escherichia coli AMP + diphosphate + Asn
-
?
additional information L-Asp-dependent ATP-diphosphate exchange in the absence of added NH4+ Escherichia coli ?
-
?
additional information no activity with glutamine Lactiplantibacillus plantarum ?
-
?
additional information no activity with glutamine Escherichia coli ?
-
?
additional information no activity with glutamine Streptococcus equinus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38 42
-
Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
Lactiplantibacillus plantarum
8 8.4
-
Escherichia coli