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Literature summary for 6.2.2.1 extracted from

  • Dong, S.; Liu, A.; Mahanta, N.; Mitchell, D.; Nair, S.
    Mechanistic basis for ribosomal peptide backbone modifications (2019), ACS Cent. Sci., 5, 842-851 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
I367F the mutant shows 74% of wild type activity Methanocaldococcus jannaschii
I367W the mutant shows 12% of wild type activity Methanocaldococcus jannaschii
I367Y the mutant shows 13% of wild type activity Methanocaldococcus jannaschii
R177K the mutant shows 5% of wild type activity Methanocaldococcus jannaschii
R177M the mutant shows 7% of wild type activity Methanocaldococcus jannaschii
R278A the mutant shows 66% of wild type activity Methanocaldococcus jannaschii
R278D the mutant shows 39% of wild type activity Methanocaldococcus jannaschii
R278K the mutant shows 104% of wild type activity Methanocaldococcus jannaschii
R86A the mutant shows 27% of wild type activity Methanocaldococcus jannaschii
R86K the mutant shows 10% of wild type activity Methanocaldococcus jannaschii
R86M the mutant shows 16% of wild type activity Methanocaldococcus jannaschii
T154A the mutant shows 4% of wild type activity Methanocaldococcus jannaschii
T366F the mutant shows 62% of wild type activity Methanocaldococcus jannaschii
W180F the mutant shows 25% of wild type activity Methanocaldococcus jannaschii
W180V the mutant shows 25% of wild type activity Methanocaldococcus jannaschii
W303A the mutant shows 30% of wild type activity Methanocaldococcus jannaschii
Y143A the mutant shows 52% of wild type activity Methanocaldococcus jannaschii
Y143F the mutant shows 105% of wild type activity Methanocaldococcus jannaschii
Y143L the mutant shows 57% of wild type activity Methanocaldococcus jannaschii
Y66A the mutant shows 19% of wild type activity Methanocaldococcus jannaschii
Y66F the mutant shows 31% of wild type activity Methanocaldococcus jannaschii
Y66G the mutant shows 30% of wild type activity Methanocaldococcus jannaschii
Y66L the mutant shows 23% of wild type activity Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0599
-
GGRLGFYGYDLQD at pH 7.5 and 37°C Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + sulfide + [methyl-coenzyme M reductase alpha-subunit McrA]-glycine Methanocaldococcus jannaschii
-
ADP + phosphate + [methyl-coenzyme M reductase alpha-subunit McrA]-thioglycine
-
?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + sulfide + GGRLGFYGYDLQD
-
Methanocaldococcus jannaschii ADP + phosphate + GGRLGFY(thioglycine)YDLQD
-
?
ATP + sulfide + [methyl-coenzyme M reductase alpha-subunit McrA]-glycine
-
Methanocaldococcus jannaschii ADP + phosphate + [methyl-coenzyme M reductase alpha-subunit McrA]-thioglycine
-
?

Synonyms

Synonyms Comment Organism
ycaO
-
Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.053
-
GGRLGFYGYDLQD at pH 7.5 and 37°C Methanocaldococcus jannaschii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.89
-
GGRLGFYGYDLQD at pH 7.5 and 37°C Methanocaldococcus jannaschii