BRENDA - Enzyme Database show
show all sequences of 6.2.1.B1

o-Succinylbenzoate:coenzyme A ligase from anthraquinone producing cell suspension cultures of Galium mollugo

Sieweke, H.J.; Leistner, E.; Phytochemistry 31, 2329-2335 (1992)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Co2+
less efficient activation than Mg2+
Galium mollugo
Mg2+
activates
Galium mollugo
Mn2+
less efficient activation than Mg2+
Galium mollugo
Ni2+
less efficient activation than Mg2+
Galium mollugo
Inhibitors
Inhibitors
Commentary
Organism
Structure
DIECA
-
Galium mollugo
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0599
-
CoA
-
Galium mollugo
0.0881
-
ATP
-
Galium mollugo
0.1158
-
o-succinylbenzoate
-
Galium mollugo
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 2-succinylbenzoate + CoA
Galium mollugo
the enzyme is involved in anthraquinone biosynthesis
ADP + phosphate + 2-succinylbenzoyl-CoA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Galium mollugo
-
-
-
Galium verum
-
-
-
Rubia tinctorum
-
-
-
Purification (Commentary)
Commentary
Organism
-
Galium mollugo
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Galium mollugo
-
cell culture
-
Galium verum
-
cell culture
-
Rubia tinctorum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000834
-
-
Galium mollugo
Storage Stability
Storage Stability
Organism
-25°C, enzyme in crude extract has a half-life of 672 h
Galium mollugo
4°C, enzyme in crude extract has a half-life of 37 h
Galium mollugo
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2-succinylbenzoate + CoA
-
649038
Galium mollugo
ADP + phosphate + 2-succinylbenzoyl-CoA
-
649038
Galium mollugo
?
ATP + 2-succinylbenzoate + CoA
the enzyme is involved in anthraquinone biosynthesis
649038
Galium mollugo
ADP + phosphate + 2-succinylbenzoyl-CoA
-
-
-
?
ATP + 2-succinylbenzoate + dephospho-CoA
26% of the activity with CoA
649038
Galium mollugo
ADP + phosphate + 2-succinylbenzoyl-CoA
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
28
-
-
Galium mollugo
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
20
-
enzyme in crude extract, half-life: 9.3 h
Galium mollugo
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
-
Galium mollugo
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Galium mollugo
phosphoralyted enzyme form, isoelectric focusing
-
7.5
Galium mollugo
non-phosphorylated enzyme form, isoelectric focusing
-
8.7
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Co2+
less efficient activation than Mg2+
Galium mollugo
Mg2+
activates
Galium mollugo
Mn2+
less efficient activation than Mg2+
Galium mollugo
Ni2+
less efficient activation than Mg2+
Galium mollugo
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
DIECA
-
Galium mollugo
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0599
-
CoA
-
Galium mollugo
0.0881
-
ATP
-
Galium mollugo
0.1158
-
o-succinylbenzoate
-
Galium mollugo
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 2-succinylbenzoate + CoA
Galium mollugo
the enzyme is involved in anthraquinone biosynthesis
ADP + phosphate + 2-succinylbenzoyl-CoA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Galium mollugo
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Galium mollugo
-
cell culture
-
Galium verum
-
cell culture
-
Rubia tinctorum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000834
-
-
Galium mollugo
Storage Stability (protein specific)
Storage Stability
Organism
-25°C, enzyme in crude extract has a half-life of 672 h
Galium mollugo
4°C, enzyme in crude extract has a half-life of 37 h
Galium mollugo
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2-succinylbenzoate + CoA
-
649038
Galium mollugo
ADP + phosphate + 2-succinylbenzoyl-CoA
-
649038
Galium mollugo
?
ATP + 2-succinylbenzoate + CoA
the enzyme is involved in anthraquinone biosynthesis
649038
Galium mollugo
ADP + phosphate + 2-succinylbenzoyl-CoA
-
-
-
?
ATP + 2-succinylbenzoate + dephospho-CoA
26% of the activity with CoA
649038
Galium mollugo
ADP + phosphate + 2-succinylbenzoyl-CoA
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
28
-
-
Galium mollugo
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
20
-
enzyme in crude extract, half-life: 9.3 h
Galium mollugo
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
-
Galium mollugo
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Galium mollugo
phosphoralyted enzyme form, isoelectric focusing
-
7.5
Galium mollugo
non-phosphorylated enzyme form, isoelectric focusing
-
8.7
Other publictions for EC 6.2.1.B1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
649038
Sieweke
-
o-Succinylbenzoate:coenzyme A ...
Galium mollugo, Galium verum, Rubia tinctorum
Phytochemistry
31
2329-2335
1992
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