Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BLR(DE3) cells and Nicotiana tabacum leaves | Medicago truncatula |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.081 | - |
oxalate | at pH 8.0, temperature not specified in the publication | Medicago truncatula |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Medicago truncatula | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + oxalate + CoA | Medicago truncatula | - |
AMP + diphosphate + oxalyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago truncatula | - |
ecotype R108 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Sephadex G-25 gel filtration | Medicago truncatula |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Medicago truncatula | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + oxalate + CoA | - |
Medicago truncatula | AMP + diphosphate + oxalyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Medicago truncatula |
Synonyms | Comment | Organism |
---|---|---|
AAE3 | - |
Medicago truncatula |
Oxalyl-CoA synthetase | - |
Medicago truncatula |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Medicago truncatula |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Medicago truncatula |
General Information | Comment | Organism |
---|---|---|
malfunction | reduction of enzyme expression increases susceptibility of Medicago truncatula to the oxalate-secreting fungal pathogen Sclerotinia sclerotiorum and results in the accumulation of druse crystals of calcium oxalate | Medicago truncatula |