Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
C219G | the mutant shows 5.6% activity compared to the wild type enzyme | Oryza sativa |
C287G | the mutant shows 25.4% activity compared to the wild type enzyme | Oryza sativa |
C363G | the mutant shows 7.2% activity compared to the wild type enzyme | Oryza sativa |
C454G | the mutant shows 44.5% activity compared to the wild type enzyme | Oryza sativa |
C473G | the mutant shows 42.9% activity compared to the wild type enzyme | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide | almost complete inhibition at 5 mM | Oryza sativa | |
5,5'-dithiobis-(2-nitrobenzoic acid) | less than 10% residual activity at 0.1 mM | Oryza sativa | |
N-ethylmaleimide | complete inhibition in Tris-HCl at 0.1 mM | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.73 | - |
oxalate | wild type enzyme, at pH 7.5 and 37°C | Oryza sativa | |
2.62 | - |
oxalate | mutant enzyme C473G, at pH 7.5 and 37°C | Oryza sativa | |
3.82 | - |
oxalate | mutant enzyme C219G, at pH 7.5 and 37°C | Oryza sativa | |
4.64 | - |
oxalate | mutant enzyme C363G, at pH 7.5 and 37°C | Oryza sativa | |
44.5 | - |
oxalate | mutant enzyme C454G, at pH 7.5 and 37°C | Oryza sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Oryza sativa | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + oxalate + CoA | Oryza sativa | the enzyme is highly specific for oxalate | AMP + diphosphate + oxalyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
cultivar Zhonghua 11 | - |
Purification (Comment) | Organism |
---|---|
nickel chelating affinity column chromatography | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf epidermis | - |
Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + oxalate + CoA | the enzyme is highly specific for oxalate | Oryza sativa | AMP + diphosphate + oxalyl-CoA | - |
? | |
additional information | no activity with malonate, succinate, malate, acetate, formate, lactate, glycolate, and glyoxylate | Oryza sativa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AAE3 | - |
Oryza sativa |
OCS | - |
Oryza sativa |
Oxalyl-CoA synthetase | - |
Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Oryza sativa |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in oxalate catabolism | Oryza sativa |