Cloned (Comment) | Organism |
---|---|
expression of GFP-tagged enzyme in onion epidermal cell cytoplasm using the constitutive 35S promoter, and from binary construct, transfected into Agrobacterium tumefaciens, GFP-tagged enzyme is transiently expressed in Nicotiana benthamiana leaf cytoplasm | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | aae3-1 and aae3-2 are null mutants are deficient in oxalyl-CoA synthetase | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.149 | - |
oxalate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Arabidopsis thaliana | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + oxalate + CoA | Arabidopsis thaliana | - |
AMP + diphosphate + oxalyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SMT7 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + oxalate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + oxalyl-CoA | - |
? | |
ATP + oxalate + CoA | specific for oxalate | Arabidopsis thaliana | AMP + diphosphate + oxalyl-CoA | - |
? | |
additional information | no activity with malonate, succinate, malate, acetate, formate, lactate, glycolate, glyoxylate, and glutarate | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AAE3 | - |
Arabidopsis thaliana |
acyl-activating enzyme3 | - |
Arabidopsis thaliana |
Oxalyl-CoA synthetase | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | activity range, profile overview | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | aae3 null mutants show increased soluble and insoluble oxalate contents compared to the wild-type enzyme, loss of AAE3 interferes with seed development affecting the integrity and function of the seed coat, phenotype, overview | Arabidopsis thaliana |
metabolism | the enzyme catalyzes the first step in oxalate degradation | Arabidopsis thaliana |
physiological function | oxalyl-CoA synthetase is required for oxalate degradation, for normal seed development, and for defense against an oxalate-producing fungal pathogen, e.g. the fungus Sclerotinia sclerotiorum | Arabidopsis thaliana |