Cloned (Comment) | Organism |
---|---|
gene YBR222C, expression of His-tagged enzyme in Escherichia coli strain BLR(DE3) | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Saccharomyces cerevisiae | |
0.02 | - |
oxalate | pH 7.5, temperature not specified in the publication | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + oxalate + CoA | Saccharomyces cerevisiae | - |
AMP + diphosphate + oxalyl-CoA | - |
? | |
ATP + oxalate + CoA | Saccharomyces cerevisiae BY4741 | - |
AMP + diphosphate + oxalyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P38137 | gene YBR222C | - |
Saccharomyces cerevisiae BY4741 | P38137 | gene YBR222C | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BLR(DE3) by nickel affinity chromatography to over 90% purity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + oxalate + CoA | - |
Saccharomyces cerevisiae | AMP + diphosphate + oxalyl-CoA | - |
? | |
ATP + oxalate + CoA | - |
Saccharomyces cerevisiae BY4741 | AMP + diphosphate + oxalyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acyl-activating enzyme 3 | - |
Saccharomyces cerevisiae |
Oxalyl-CoA synthetase | - |
Saccharomyces cerevisiae |
ScAAE3 | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | activity range, profile overview | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |
Organism | Comment | Expression |
---|---|---|
Saccharomyces cerevisiae | oxalate induced the enzyme, quantitative RT-PCR reveals that ScAAE3 transcript levels are induced within min of exposure peaking at about 1 h before declining back to steady state levels | up |
General Information | Comment | Organism |
---|---|---|
malfunction | a Scaae3 deletion mutant strain shows highly reduced oxalate degradation activity decreasing recovery from exposure to oxalate, e.g. by exposure to oxalate-secreting microbes, and to oxidative stress, e.g. by H2O2 | Saccharomyces cerevisiae |
physiological function | the enzyme catalyzes the first step in a pathway of oxalate degradation to protect the cell against the harmful effects of oxalate derived from an endogenous process or an environmental source | Saccharomyces cerevisiae |