Literature summary for 6.2.1.64 extracted from

Bruzzese, F.J.; Tsu, C.A.; Ma, J.; Loke, H.K.; Wu, D.; Li, Z.; Tayber, O.; Dick, L.R.
Development of a charcoal paper adenosine triphosphate pyrophosphate exchange assay kinetic characterization of NEDD8 activating enzyme (2009), Anal. Biochem., 394, 24-29 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Sf9 insect cells	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.011	-	diphosphate	at pH 7.5 and 37°C	Homo sapiens
0.175	-	ATP	at pH 7.5 and 37°C	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	10 mM used in assay conditions	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine	Homo sapiens	-	ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine	-	r
ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine	Homo sapiens	-	AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine	-	Homo sapiens	ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine	-	r
ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine	-	Homo sapiens	AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine	-	r
additional information	the enzyme exhibits very low activity with ubiquitin, SUMO1, SUMO2, MAPLC3A, GABRAP, GATE-16, Ufm-1, FAT10 and ISG-15	Homo sapiens	?	-	-

Synonyms

Synonyms	Comment	Organism
APP-BP-1	-	Homo sapiens
NAE	-	Homo sapiens
NAEalpha	-	Homo sapiens
NAEbeta	-	Homo sapiens
NEDD8 activating enzyme	-	Homo sapiens
Uba3	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.49	-	ATP	at pH 7.5 and 37°C	Homo sapiens
0.54	-	diphosphate	at pH 7.5 and 37°C	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

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Release 2023.1 (January 2023)