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Literature summary for 6.2.1.62 extracted from
- Characterization and analysis of early enzymes for petrobactin biosynthesis in Bacillus anthracis (2007), Biochemistry, 46, 4147-4157 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BLR(DE3) | Bacillus anthracis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,4-dihydroxybenzoate | substrate inhibition | Bacillus anthracis |  |
| ATP | substrate inhibition | Bacillus anthracis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0031 | - |
3,4-dihydroxybenzoate | pH 7.5, temperature not specified in the publication | Bacillus anthracis | |
| 0.216 | - |
ATP | pH 7.5, temperature not specified in the publication | Bacillus anthracis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
His-tagged enzyme, SDS-PAGE | Bacillus anthracis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3,4-dihydroxybenzoate + holo-[aryl-carrier protein] | Bacillus anthracis | the enzyme is involved in petrobactin biosynthesis | AMP + diphosphate + 3,4-dihydroxybenzoyl-[aryl-carrier protein] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus anthracis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus anthracis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3,4-dihydroxybenzoyl)adenylate + holo-[aryl-carrier protein] | the adenylation domain of the enzyme catalyses the activation of 3,4-dihydroxybenzoate to (3,4-dihydroxybenzoyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of an aryl-carrier protein domain | Bacillus anthracis | AMP + 3,4-dihydroxybenzoyl-[aryl-carrier protein] | - |
? | |
| ATP + 3,4-dihydroxybenzoate | the adenylation domain of the enzyme catalyses the activation of 3,4-dihydroxybenzoate to (3,4-dihydroxybenzoyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of an aryl-carrier protein domain | Bacillus anthracis | diphosphate + (3,4-dihydroxybenzoyl)adenylate | - |
? | |
| ATP + 3,4-dihydroxybenzoate + holo-[aryl-carrier protein] | the enzyme is involved in petrobactin biosynthesis | Bacillus anthracis | AMP + diphosphate + 3,4-dihydroxybenzoyl-[aryl-carrier protein] | - |
? | |
| ATP + 3,4-dihydroxybenzoate + holo-[aryl-carrier protein] | the adenylation domain of the enzyme catalyses the activation of 3,4-dihydroxybenzoate to (3,4-dihydroxybenzoyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of an aryl-carrier protein domain | Bacillus anthracis | AMP + diphosphate + 3,4-dihydroxybenzoyl-[aryl-carrier protein] | - |
? | |
| ATP + 3,5-dihydroxybenzoic acid + holo-[aryl-carrier protein] | about 20% of the activity compared to 3,4-dihydroxybenzoate | Bacillus anthracis | AMP + diphosphate + 3,5-dihydroxybenzoyl-[aryl-carrier protein] | - |
? | |
| ATP + 3-chloro-4-hydroxybenzoic acid + holo-[aryl-carrier protein] | about 5% of the activity compared to 3,4-dihydroxybenzoate | Bacillus anthracis | AMP + diphosphate + 3-chloro-4-hydroxybenzoyl-[aryl-carrier protein] | - |
? | |
| ATP + 3-hydroxybenzoic acid + holo-[aryl-carrier protein] | about 5% of thze activity compared to 3,4-dihydroxybenzoate | Bacillus anthracis | AMP + diphosphate + 3-hydroxybenzoyl-[aryl-carrier protein] | - |
? | |
| ATP + 4-fluoro-3-hydroxybenzoic acid + holo-[aryl-carrier protein] | about 5% of the activity compared to 3,4-dihydroxybenzoate | Bacillus anthracis | AMP + diphosphate + 4-fluoro-3-hydroxybenzoyl-[aryl-carrier protein] | - |
? | |
| ATP + 4-hydroxybenzoic acid + holo-[aryl-carrier protein] | about 60% of the activity compared to 3,4-dihydroxybenzoate | Bacillus anthracis | AMP + diphosphate + 4-hydroxybenzoyl-[aryl-carrier protein] | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47000, His-tagged enzyme, SDS-PAGE | Bacillus anthracis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3,4-dihydroxybenzoic acid-AMP ligase | - |
Bacillus anthracis |
| asbC | - |
Bacillus anthracis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.118 | - |
3,4-dihydroxybenzoate | pH 7.5, temperature not specified in the publication | Bacillus anthracis | |
| 0.152 | - |
ATP | pH 7.5, temperature not specified in the publication | Bacillus anthracis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
3,4-dihydroxybenzoate | pH 7.5, temperature not specified in the publication | Bacillus anthracis | |
| 54 | - |
ATP | pH 7.5, temperature not specified in the publication | Bacillus anthracis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in petrobactin biosynthesis | Bacillus anthracis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
ATP | pH 7.5, temperature not specified in the publication | Bacillus anthracis | |
| 30.06 | - |
3,4-dihydroxybenzoate | pH 7.5, temperature not specified in the publication | Bacillus anthracis | |
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