Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BLR(DE3) | Bacillus anthracis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dihydroxybenzoate | substrate inhibition | Bacillus anthracis | |
ATP | substrate inhibition | Bacillus anthracis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
3,4-dihydroxybenzoate | pH 7.5, temperature not specified in the publication | Bacillus anthracis | |
0.216 | - |
ATP | pH 7.5, temperature not specified in the publication | Bacillus anthracis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
His-tagged enzyme, SDS-PAGE | Bacillus anthracis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3,4-dihydroxybenzoate + holo-[aryl-carrier protein] | Bacillus anthracis | the enzyme is involved in petrobactin biosynthesis | AMP + diphosphate + 3,4-dihydroxybenzoyl-[aryl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus anthracis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3,4-dihydroxybenzoyl)adenylate + holo-[aryl-carrier protein] | the adenylation domain of the enzyme catalyses the activation of 3,4-dihydroxybenzoate to (3,4-dihydroxybenzoyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of an aryl-carrier protein domain | Bacillus anthracis | AMP + 3,4-dihydroxybenzoyl-[aryl-carrier protein] | - |
? | |
ATP + 3,4-dihydroxybenzoate | the adenylation domain of the enzyme catalyses the activation of 3,4-dihydroxybenzoate to (3,4-dihydroxybenzoyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of an aryl-carrier protein domain | Bacillus anthracis | diphosphate + (3,4-dihydroxybenzoyl)adenylate | - |
? | |
ATP + 3,4-dihydroxybenzoate + holo-[aryl-carrier protein] | the enzyme is involved in petrobactin biosynthesis | Bacillus anthracis | AMP + diphosphate + 3,4-dihydroxybenzoyl-[aryl-carrier protein] | - |
? | |
ATP + 3,4-dihydroxybenzoate + holo-[aryl-carrier protein] | the adenylation domain of the enzyme catalyses the activation of 3,4-dihydroxybenzoate to (3,4-dihydroxybenzoyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of an aryl-carrier protein domain | Bacillus anthracis | AMP + diphosphate + 3,4-dihydroxybenzoyl-[aryl-carrier protein] | - |
? | |
ATP + 3,5-dihydroxybenzoic acid + holo-[aryl-carrier protein] | about 20% of the activity compared to 3,4-dihydroxybenzoate | Bacillus anthracis | AMP + diphosphate + 3,5-dihydroxybenzoyl-[aryl-carrier protein] | - |
? | |
ATP + 3-chloro-4-hydroxybenzoic acid + holo-[aryl-carrier protein] | about 5% of the activity compared to 3,4-dihydroxybenzoate | Bacillus anthracis | AMP + diphosphate + 3-chloro-4-hydroxybenzoyl-[aryl-carrier protein] | - |
? | |
ATP + 3-hydroxybenzoic acid + holo-[aryl-carrier protein] | about 5% of thze activity compared to 3,4-dihydroxybenzoate | Bacillus anthracis | AMP + diphosphate + 3-hydroxybenzoyl-[aryl-carrier protein] | - |
? | |
ATP + 4-fluoro-3-hydroxybenzoic acid + holo-[aryl-carrier protein] | about 5% of the activity compared to 3,4-dihydroxybenzoate | Bacillus anthracis | AMP + diphosphate + 4-fluoro-3-hydroxybenzoyl-[aryl-carrier protein] | - |
? | |
ATP + 4-hydroxybenzoic acid + holo-[aryl-carrier protein] | about 60% of the activity compared to 3,4-dihydroxybenzoate | Bacillus anthracis | AMP + diphosphate + 4-hydroxybenzoyl-[aryl-carrier protein] | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, His-tagged enzyme, SDS-PAGE | Bacillus anthracis |
Synonyms | Comment | Organism |
---|---|---|
3,4-dihydroxybenzoic acid-AMP ligase | - |
Bacillus anthracis |
asbC | - |
Bacillus anthracis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.118 | - |
3,4-dihydroxybenzoate | pH 7.5, temperature not specified in the publication | Bacillus anthracis | |
0.152 | - |
ATP | pH 7.5, temperature not specified in the publication | Bacillus anthracis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
3,4-dihydroxybenzoate | pH 7.5, temperature not specified in the publication | Bacillus anthracis | |
54 | - |
ATP | pH 7.5, temperature not specified in the publication | Bacillus anthracis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in petrobactin biosynthesis | Bacillus anthracis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
ATP | pH 7.5, temperature not specified in the publication | Bacillus anthracis | |
30.06 | - |
3,4-dihydroxybenzoate | pH 7.5, temperature not specified in the publication | Bacillus anthracis |