BRENDA - Enzyme Database show
show all sequences of 6.2.1.5

Thermostable succinyl-coenzyme A synthetase from Nitrosomonas europaea ATCC 25978: purification and properties

Kondo, H.; Ohmori, T.; Shibata, H.; Sase, K.; Takahashi, R.; Tokuyama, T.; J. Ferment. Bioeng. 79, 499-502 (1995)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
0.1 mM
Nitrosomonas europaea
SDS
1.0 mM
Nitrosomonas europaea
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
CoA
-
Nitrosomonas europaea
0.4
-
ATP
-
Nitrosomonas europaea
5
-
succinate
succinate
Nitrosomonas europaea
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
1-100 mM activates
Nitrosomonas europaea
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
2 * 29000 (alpha) + 2 * 36000 (beta), SDS-PAGE
Nitrosomonas europaea
36000
-
2 * 29000 (alpha) + 2 * 36000 (beta), SDS-PAGE
Nitrosomonas europaea
130000
-
gel filtration
Nitrosomonas europaea
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ADP + phosphate + succinyl-CoA
Nitrosomonas europaea
-
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nitrosomonas europaea
-
strain ATCC 25978
-
Purification (Commentary)
Commentary
Organism
-
Nitrosomonas europaea
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + phosphate + succinyl-CoA
-
816
Nitrosomonas europaea
?
-
-
-
-
ATP + succinate + CoA
specific for ATP
816
Nitrosomonas europaea
ADP + phosphate + succinyl-CoA
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
2 * 29000 (alpha) + 2 * 36000 (beta), SDS-PAGE
Nitrosomonas europaea
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Nitrosomonas europaea
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
pH 8.0-10.0, 30 min, stable
Nitrosomonas europaea
70
-
pH 7.5, half-life: 60 min
Nitrosomonas europaea
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Nitrosomonas europaea
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
8
10
50°C, 30 min stable
Nitrosomonas europaea
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
0.1 mM
Nitrosomonas europaea
SDS
1.0 mM
Nitrosomonas europaea
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
CoA
-
Nitrosomonas europaea
0.4
-
ATP
-
Nitrosomonas europaea
5
-
succinate
succinate
Nitrosomonas europaea
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
1-100 mM activates
Nitrosomonas europaea
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
2 * 29000 (alpha) + 2 * 36000 (beta), SDS-PAGE
Nitrosomonas europaea
36000
-
2 * 29000 (alpha) + 2 * 36000 (beta), SDS-PAGE
Nitrosomonas europaea
130000
-
gel filtration
Nitrosomonas europaea
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ADP + phosphate + succinyl-CoA
Nitrosomonas europaea
-
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Nitrosomonas europaea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + phosphate + succinyl-CoA
-
816
Nitrosomonas europaea
?
-
-
-
-
ATP + succinate + CoA
specific for ATP
816
Nitrosomonas europaea
ADP + phosphate + succinyl-CoA
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
2 * 29000 (alpha) + 2 * 36000 (beta), SDS-PAGE
Nitrosomonas europaea
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Nitrosomonas europaea
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
pH 8.0-10.0, 30 min, stable
Nitrosomonas europaea
70
-
pH 7.5, half-life: 60 min
Nitrosomonas europaea
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Nitrosomonas europaea
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
8
10
50°C, 30 min stable
Nitrosomonas europaea
Other publictions for EC 6.2.1.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745783
Huang
Succinyl-CoA synthetase (SUCL ...
Homo sapiens
Mol. Genet. Metab.
120
213-222
2017
-
-
-
-
2
-
-
1
-
-
-
2
-
2
-
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-
-
-
2
-
-
2
-
-
-
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-
-
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2
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2
-
2
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1
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-
2
-
-
-
-
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2
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
746481
Zhao
Loss of succinyl-CoA synthase ...
Mus musculus
Sci. Rep.
7
7169
2017
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
1
-
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-
1
-
-
-
-
-
-
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1
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-
2
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
750677
Zhong
Differential gene expression ...
Pleurotus ostreatus
Fungal Biol.
121
1025-1036
2017
-
-
-
-
-
-
-
-
-
-
-
1
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1
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1
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1
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1
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-
-
-
-
-
-
-
1
-
-
1
-
-
744272
Kacso
Two transgenic mouse models f ...
Mus musculus
Biochem. J.
473
3463-3485
2016
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
3
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
745500
Carrozzo
Succinate-CoA ligase deficien ...
Homo sapiens
J. Inherit. Metab. Dis.
39
243-252
2016
-
-
-
-
6
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
746174
Gomes
Succinyl-CoA synthetase New a ...
Bartonella bacilliformis
PLoS Negl. Trop. Dis.
10
e0004989
2016
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726747
Nolte
Novel characteristics of succi ...
Advenella mimigardefordensis, Advenella mimigardefordensis DPN7, Alcanivorax borkumensis, Alcanivorax borkumensis SK2, Escherichia coli K-12, Escherichia coli K-12 BL21
Appl. Environ. Microbiol.
80
166-176
2014
-
-
3
-
-
-
-
20
-
-
-
24
-
17
-
-
3
-
-
-
3
-
66
-
3
-
-
20
3
-
-
8
-
-
-
-
-
6
16
-
-
-
-
-
-
40
-
-
-
24
-
-
-
6
-
-
6
-
66
-
6
-
-
40
6
-
-
-
-
1
2
-
20
40
745498
Luis
Valproyl-CoA inhibits the act ...
Homo sapiens
J. Inherit. Metab. Dis.
37
353-357
2014
-
-
-
-
-
-
3
1
1
-
-
2
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
1
1
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728078
Luis
Valproyl-CoA inhibits the acti ...
Homo sapiens
J. Inherit. Metab. Dis.
37
353-357
2013
-
-
-
-
-
-
3
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
727919
Bishop
X-linked sideroblastic anemia ...
Homo sapiens
J. Biol. Chem.
287
28943-28955
2012
-
1
1
-
-
-
-
-
1
-
-
1
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
714342
Miller
The interplay between SUCLA2, ...
Homo sapiens
Biochim. Biophys. Acta
1812
625-629
2011
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
715381
Schuermann
Novel reaction of succinyl coe ...
Advenella mimigardefordensis, Advenella mimigardefordensis DPN7T
J. Bacteriol.
193
3078-3089
2011
-
-
1
-
1
-
-
5
-
2
-
4
-
6
-
-
1
-
-
-
-
-
10
-
1
-
-
-
1
-
-
1
-
-
-
-
-
2
2
-
2
-
-
-
-
10
-
4
-
4
-
-
-
2
-
-
-
-
10
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
727749
Ramos-Vera
Regulation of autotrophic CO2 ...
Pyrobaculum neutrophilum, Pyrobaculum neutrophilum DSM 2338
J. Bacteriol.
192
5329-5340
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
5
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
1
-
-
-
-
-
-
-
1
1
1
1
-
-
702332
Phillips
Succinyl-CoA synthetase is a p ...
Sus scrofa
Biochemistry
48
7140-7149
2009
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687185
Fleige
Localisation of gluconeogenesi ...
Toxoplasma gondii
Int. J. Parasitol.
38
1121-1132
2008
-
-
1
-
-
-
-
-
2
1
-
1
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
692865
Mullins
A specialized citric acid cycl ...
Acetobacter aceti
J. Bacteriol.
190
4933-4940
2008
-
-
1
-
-
-
1
5
-
1
2
1
-
1
-
-
1
1
-
-
4
-
3
1
1
-
-
4
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
5
-
1
2
1
-
-
-
1
-
-
4
-
3
1
1
-
-
4
1
-
-
-
-
-
-
-
-
-
693488
Ostergaard
Disorders caused by deficiency ...
Homo sapiens
J. Inherit. Metab. Dis.
31
226-229
2008
-
-
-
-
1
-
-
-
2
1
-
5
-
4
-
-
-
-
-
5
-
-
6
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
5
-
3
-
-
-
-
-
3
1
-
5
-
-
-
-
-
11
-
-
6
2
-
-
-
-
-
-
-
-
-
1
2
-
-
-
693743
Gao
Drosophila ortholog of succiny ...
Drosophila melanogaster
J. Neurophysiol.
99
2736-2740
2008
-
-
1
-
-
-
-
-
-
1
-
2
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
694228
Hamblin
Localization and nucleotide sp ...
Blastocystis sp.
Mol. Microbiol.
68
1395-1405
2008
-
-
1
-
-
-
-
-
1
1
3
1
-
1
-
-
1
-
-
-
-
-
2
2
1
-
-
-
1
-
-
1
-
2
-
-
-
1
1
-
-
-
-
-
-
-
1
1
3
1
-
-
-
1
-
-
-
-
2
2
1
-
-
-
1
-
-
2
-
-
-
-
-
-
671076
Hidber
Participation of Cys123alpha o ...
Escherichia coli
Acta Crystallogr. Sect. D
D63
876-884
2007
-
-
-
-
4
-
-
14
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
671128
Joyce
Cloning, expression, purificat ...
Thermus aquaticus
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2
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10
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-
-
-
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2
2
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-
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-
-
2
-
-
-
10
-
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-
-
-
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-
788
Weitzman
Pattern of nucleotide utilizat ...
Alcaligenes faecalis, Bacillus megaterium, Brevibacterium linens, Escherichia coli, Geobacillus stearothermophilus, Klebsiella aerogenes, Kurthia zopfii, More, Paracoccus denitrificans, Pimelobacter simplex, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas stutzeri, Rhodobacter sphaeroides, Salmonella enterica subsp. arizonae, Serratia marcescens, Thermus aquaticus
FEBS Lett.
143
237-240
1982
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17
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29
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-
29
-
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-
-
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-
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-
-
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-
803
Hamilton
An ATP-dependent succinic thio ...
Gallus gallus, Pigeon
FEBS Lett.
123
252-254
1981
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2
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2
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9
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2
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2
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9
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2
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-
-
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-
814
Wider de Xifra
Porphyrin biosynthesis: immobi ...
Glycine max
Biochim. Biophys. Acta
523
245-249
1978
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1
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4
-
1
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1
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1
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1
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2
2
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2
2
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1
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4
-
1
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-
1
-
-
1
-
2
2
-
-
2
2
-
-
-
-
-
-
-
-
815
Nishimura
Inactivation of Escherichia co ...
Escherichia coli
Biochem. Biophys. Res. Commun.
69
1057-1064
1976
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1
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1
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1
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1
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1
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-
804
Pearson
Isolation of the alpha and bet ...
Escherichia coli
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250
4451-4455
1975
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1
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1
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1
1
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1
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1
1
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806
Thomas
Studies on the metabolism of A ...
Escherichia coli
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163
530-536
1974
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1
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1
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2
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1
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2
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807
Krebs
Some physical parameters of su ...
Escherichia coli
Can. J. Biochem.
52
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1974
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3
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1
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1
2
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3
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1
2
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-
808
Hansford
An adenine nucleotide-linked s ...
Calliphoridae
FEBS Lett.
31
317-320
1973
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4
-
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1
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1
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1
1
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1
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4
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1
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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-
809
Moffet
Succinyl coenzyme A synthetase ...
Escherichia coli
J. Biol. Chem.
247
8139-8144
1972
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-
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-
2
-
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1
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1
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-
2
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-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
810
Murakami
Nucleotide specificity of Esch ...
Escherichia coli
J. Biol. Chem.
247
6247-6252
1972
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1
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4
-
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4
-
-
-
-
-
-
-
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-
805
Wider
Porphyrin biosynthesis in soyb ...
Glycine max
Enzymologia
41
217-231
1971
3
-
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-
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-
25
2
-
2
1
1
-
1
-
-
1
-
-
1
1
1
3
-
1
-
-
-
1
1
-
-
-
-
-
3
-
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-
-
-
-
-
25
-
2
-
2
1
1
-
-
-
1
-
1
1
1
3
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
811
Hager
-
Succinyl CoA synthetase ...
Escherichia coli, Spinacia oleracea, Sus scrofa
The Enzymes, 2nd Ed (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. )
6
387-399
1962
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3
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1
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3
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1
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5
-
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3
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1
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1
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5
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-