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Literature summary for 6.2.1.5 extracted from

  • Kondo, H.; Ohmori, T.; Shibata, H.; Sase, K.; Takahashi, R.; Tokuyama, T.
    Thermostable succinyl-coenzyme A synthetase from Nitrosomonas europaea ATCC 25978: purification and properties (1995), J. Ferment. Bioeng., 79, 499-502.
No PubMed abstract available
Search for more literature for 6.2.1.5

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 0.1 mM Nitrosomonas europaea
SDS 1.0 mM Nitrosomonas europaea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 CoA
-
 Nitrosomonas europaea
0.4
-
 ATP
-
 Nitrosomonas europaea
5
-
 succinate succinate Nitrosomonas europaea

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1-100 mM activates Nitrosomonas europaea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 2 * 29000 (alpha) + 2 * 36000 (beta), SDS-PAGE Nitrosomonas europaea
36000
-
 2 * 29000 (alpha) + 2 * 36000 (beta), SDS-PAGE Nitrosomonas europaea
130000
-
 gel filtration Nitrosomonas europaea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + phosphate + succinyl-CoA Nitrosomonas europaea
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Nitrosomonas europaea
-
 strain ATCC 25978
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nitrosomonas europaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphate + succinyl-CoA
-
 Nitrosomonas europaea ?
-
 ?
ATP + succinate + CoA specific for ATP Nitrosomonas europaea ADP + phosphate + succinyl-CoA
-
 ?

Subunits

Subunits Comment Organism
tetramer 2 * 29000 (alpha) + 2 * 36000 (beta), SDS-PAGE Nitrosomonas europaea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Nitrosomonas europaea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 8.0-10.0, 30 min, stable Nitrosomonas europaea
70
-
 pH 7.5, half-life: 60 min Nitrosomonas europaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Nitrosomonas europaea

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10 50°C, 30 min stable Nitrosomonas europaea