Literature summary for 6.2.1.49 extracted from

Goyal, A.; Yousuf, M.; Rajakumara, E.; Arora, P.; Gokhale, R.; Sankaranarayanan, R.
Crystallization and preliminary X-ray crystallographic studies of the N-terminal domain of FadD28, a fatty-acyl AMP ligase from Mycobacterium tuberculosis (2006), Acta Crystallogr. Sect. F, 62, 350-352.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 20.3% (w/v) polyethylene glycol 3350, 0.1 M Na MES pH 6.0, 5 mM 2-mercaptoethanol and 0.13 M lithium sulfate	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + a long-chain fatty acid + holo-[mycocerosate synthase]	Mycobacterium tuberculosis	-	AMP + diphosphate + a long-chain acyl-[mycocerosate synthase]	-	?
ATP + a long-chain fatty acid + holo-[mycocerosate synthase]	Mycobacterium tuberculosis H37Rv	-	AMP + diphosphate + a long-chain acyl-[mycocerosate synthase]	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WQ59	-	-
Mycobacterium tuberculosis H37Rv	P9WQ59	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex 75 gel filtration	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + a long-chain fatty acid + holo-[mycocerosate synthase]	-	Mycobacterium tuberculosis	AMP + diphosphate + a long-chain acyl-[mycocerosate synthase]	-	?
ATP + a long-chain fatty acid + holo-[mycocerosate synthase]	-	Mycobacterium tuberculosis H37Rv	AMP + diphosphate + a long-chain acyl-[mycocerosate synthase]	-	?

Synonyms

Synonyms	Comment	Organism
fadD28	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)