BRENDA - Enzyme Database
show all sequences of 6.2.1.49

Crystallization and preliminary X-ray crystallographic studies of the N-terminal domain of FadD28, a fatty-acyl AMP ligase from Mycobacterium tuberculosis

Goyal, A.; Yousuf, M.; Rajakumara, E.; Arora, P.; Gokhale, R.; Sankaranarayanan, R.; Acta Crystallogr. Sect. F 62, 350-352 (2006)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Mycobacterium tuberculosis
Crystallization (Commentary)
Crystallization (Commentary)
Organism
hanging drop vapor diffusion method, using 20.3% (w/v) polyethylene glycol 3350, 0.1 M Na MES pH 6.0, 5 mM 2-mercaptoethanol and 0.13 M lithium sulfate
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + a long-chain fatty acid + holo-[mycocerosate synthase]
Mycobacterium tuberculosis
-
AMP + diphosphate + a long-chain acyl-[mycocerosate synthase]
-
-
?
ATP + a long-chain fatty acid + holo-[mycocerosate synthase]
Mycobacterium tuberculosis H37Rv
-
AMP + diphosphate + a long-chain acyl-[mycocerosate synthase]
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Mycobacterium tuberculosis
P9WQ59
-
-
Mycobacterium tuberculosis H37Rv
P9WQ59
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni-NTA column chromatography and Superdex 75 gel filtration
Mycobacterium tuberculosis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + a long-chain fatty acid + holo-[mycocerosate synthase]
-
739802
Mycobacterium tuberculosis
AMP + diphosphate + a long-chain acyl-[mycocerosate synthase]
-
-
-
?
ATP + a long-chain fatty acid + holo-[mycocerosate synthase]
-
739802
Mycobacterium tuberculosis H37Rv
AMP + diphosphate + a long-chain acyl-[mycocerosate synthase]
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
fadD28
-
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Mycobacterium tuberculosis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, using 20.3% (w/v) polyethylene glycol 3350, 0.1 M Na MES pH 6.0, 5 mM 2-mercaptoethanol and 0.13 M lithium sulfate
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + a long-chain fatty acid + holo-[mycocerosate synthase]
Mycobacterium tuberculosis
-
AMP + diphosphate + a long-chain acyl-[mycocerosate synthase]
-
-
?
ATP + a long-chain fatty acid + holo-[mycocerosate synthase]
Mycobacterium tuberculosis H37Rv
-
AMP + diphosphate + a long-chain acyl-[mycocerosate synthase]
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography and Superdex 75 gel filtration
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + a long-chain fatty acid + holo-[mycocerosate synthase]
-
739802
Mycobacterium tuberculosis
AMP + diphosphate + a long-chain acyl-[mycocerosate synthase]
-
-
-
?
ATP + a long-chain fatty acid + holo-[mycocerosate synthase]
-
739802
Mycobacterium tuberculosis H37Rv
AMP + diphosphate + a long-chain acyl-[mycocerosate synthase]
-
-
-
?
Other publictions for EC 6.2.1.49
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738537
Vergnolle
Biosynthesis of cell envelope- ...
Mycobacterium marinum
J. Bacteriol.
197
1040-1050
2015
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1
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1
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1
1
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740948
Menendez-Bravo
Expanding the chemical diversi ...
Mycobacterium tuberculosis
Metab. Eng.
24
97-106
2014
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1
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1
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3
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1
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1
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1
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1
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740805
Chuang
Single-nucleotide polymorphism ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Clin. Microbiol.
48
4245-4247
2010
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-
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2
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3
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2
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1
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2
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2
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-
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-
739802
Goyal
Crystallization and preliminar ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Acta Crystallogr. Sect. F
62
350-352
2006
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-
1
1
-
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-
-
-
-
2
-
4
-
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1
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2
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1
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1
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1
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2
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1
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2
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740601
Sirakova
Regulation of expression of ma ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis variant bovis
J. Bacteriol.
184
6796-6802
2002
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-
1
-
-
-
-
-
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1
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8
-
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1
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2
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1
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1
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1
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1
1
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486139
Li
cDNA-RNA subtractive hybridiza ...
Mycobacterium tuberculosis variant bovis
Microbiology
147
2293-2305
2001
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7
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1
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