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Literature summary for 6.2.1.48 extracted from

  • Bernal, V.; Arense, P.; Blatz, V.; Mandrand-Berthelot, M.A.; Canovas, M.; Iborra, J.L.
    Role of betaine:CoA ligase (CaiC) in the activation of betaines and the transfer of coenzyme A in Escherichia coli (2008), J. Appl. Microbiol., 105, 42-50.
    View publication on PubMed
Search for more literature for 6.2.1.48

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Escherichia coli
expression in Escherichia coli Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-carnitine + CoA Escherichia coli
-
 AMP + diphosphate + L-carnitinyl-CoA
-
 ir

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli P31552
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + crotonobetaine + CoA
-
 Escherichia coli AMP + diphosphate + crotonobetainyl-CoA
-
 ?
ATP + crotonobetaine + CoA
-
 Escherichia coli AMP + diphosphate + crotonobetainyl-CoA
-
 ir
ATP + D-carnitine + CoA
-
 Escherichia coli AMP + diphosphate + D-carnitinyl-CoA
-
 ?
ATP + D-carnitine + CoA
-
 Escherichia coli AMP + diphosphate + D-carnitinyl-CoA
-
 ir
ATP + gamma-butyrobetaine + CoA
-
 Escherichia coli AMP + diphosphate + gamma-butyrobetainyl-CoA
-
 ?
ATP + gamma-butyrobetaine + CoA
-
 Escherichia coli AMP + diphosphate + gamma-butyrobetainyl-CoA
-
 ir
ATP + L-carnitine + CoA
-
 Escherichia coli AMP + diphosphate + L-carnitinyl-CoA
-
 ?
ATP + L-carnitine + CoA
-
 Escherichia coli AMP + diphosphate + L-carnitinyl-CoA
-
 ir
additional information no substrate: glycinebetaine, butanoate, crotonate, gamma-amino-butanoate and gamma-amino-3-hydroxybutanoate. CaiC exhibits an in vitro CoA-transferase-like activity Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
betaine-CoA ligase
-
 Escherichia coli
caiC
-
 Escherichia coli

General Information

General Information Comment Organism
physiological function overexpression of CaiC in Escherichia coli enhances the biotransformation of crotonobetaine to L-carnitine nearly 20fold, the yield reaching up to 30% (with growing cells). Higher yields are obtained using resting cells (up to 60%), even when D-carnitine is used as substrate Escherichia coli