Cloned (Comment) | Organism |
---|---|
expression in baculoviral system | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
[ubiquitin carrier protein Ubc4]-L-cysteine | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00017 | - |
ubiquitin | pH 7.5, 25°C | Homo sapiens | |
0.00017 | - |
Oregon Green-labeled ubiquitin | pH 7.5, 25°C | Homo sapiens | |
0.0047 | - |
ATP | pH 7.5, 25°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
112000 | - |
analytical ultracentrifugation | Homo sapiens |
117300 | - |
1 * 117300, calculated | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Oregon Green-labeled ubiquitin + [ubiquitin-activating protein E1]-L-cysteine | enzyme efficiently accepts ubiquitin substrate fluorescently labeled by Oregon Green | Homo sapiens | AMP + diphosphate + [ubiquitin-activating protein E1]-S-(Oregon Green-labeled ubiquitinyl)-L-cysteine | - |
? | |
ATP + ubiquitin + [ubiquitin-activating protein E1]-L-cysteine | - |
Homo sapiens | AMP + diphosphate + [ubiquitin-activating protein E1]-S-ubiquitinyl-L-cysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 117300, calculated | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
[ubiquitin carrier protein Ubc4]-L-cysteine | pH 7.5, 25°C | Homo sapiens |