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Literature summary for 6.2.1.40 extracted from

  • Hawkins, A.B.; Adams, M.W.; Kelly, R.M.
    Conversion of 4-hydroxybutyrate to acetyl coenzyme A and its anapleurosis in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway (2014), Appl. Environ. Microbiol., 80, 2536-2545.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinantly expressed in Escherichia coli Metallosphaera sedula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
4-hydroxybutanoate pH 7.5, 70°C Metallosphaera sedula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-hydroxybutanoate + CoA Metallosphaera sedula the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway. The flux between the succinate and acetyl-CoA branches in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway is governed by 4-hydroxybutyrate-CoA ligase, possibly regulated posttranslationally by the protein acetyltransferase AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
?
ATP + 4-hydroxybutanoate + CoA Metallosphaera sedula DSM 5348 the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway. The flux between the succinate and acetyl-CoA branches in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway is governed by 4-hydroxybutyrate-CoA ligase, possibly regulated posttranslationally by the protein acetyltransferase AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Metallosphaera sedula A4YDT1
-
-
Metallosphaera sedula DSM 5348 A4YDT1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-hydroxybutanoate + CoA
-
Metallosphaera sedula AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
?
ATP + 4-hydroxybutanoate + CoA the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway. The flux between the succinate and acetyl-CoA branches in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway is governed by 4-hydroxybutyrate-CoA ligase, possibly regulated posttranslationally by the protein acetyltransferase Metallosphaera sedula AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
?
ATP + 4-hydroxybutanoate + CoA
-
Metallosphaera sedula DSM 5348 AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
?
ATP + 4-hydroxybutanoate + CoA the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway. The flux between the succinate and acetyl-CoA branches in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway is governed by 4-hydroxybutyrate-CoA ligase, possibly regulated posttranslationally by the protein acetyltransferase Metallosphaera sedula DSM 5348 AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
?

Synonyms

Synonyms Comment Organism
4-hydroxybutyrate-CoA ligase (AMP forming)
-
Metallosphaera sedula
Msed_0406 locus name Metallosphaera sedula

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Metallosphaera sedula

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
4-hydroxybutanoate pH 7.5, 70°C Metallosphaera sedula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Metallosphaera sedula

General Information

General Information Comment Organism
physiological function the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway. The flux between the succinate and acetyl-CoA branches in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway is governed by 4-hydroxybutyrate-CoA ligase, possibly regulated posttranslationally by the protein acetyltransferase Metallosphaera sedula

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.9
-
4-hydroxybutanoate pH 7.5, 70°C Metallosphaera sedula