Cloned (Comment) | Organism |
---|---|
recombinantly expressed in Escherichia coli | Metallosphaera sedula |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
4-hydroxybutanoate | pH 7.5, 70°C | Metallosphaera sedula |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-hydroxybutanoate + CoA | Metallosphaera sedula | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway. The flux between the succinate and acetyl-CoA branches in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway is governed by 4-hydroxybutyrate-CoA ligase, possibly regulated posttranslationally by the protein acetyltransferase | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
ATP + 4-hydroxybutanoate + CoA | Metallosphaera sedula DSM 5348 | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway. The flux between the succinate and acetyl-CoA branches in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway is governed by 4-hydroxybutyrate-CoA ligase, possibly regulated posttranslationally by the protein acetyltransferase | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metallosphaera sedula | A4YDT1 | - |
- |
Metallosphaera sedula DSM 5348 | A4YDT1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-hydroxybutanoate + CoA | - |
Metallosphaera sedula | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
ATP + 4-hydroxybutanoate + CoA | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway. The flux between the succinate and acetyl-CoA branches in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway is governed by 4-hydroxybutyrate-CoA ligase, possibly regulated posttranslationally by the protein acetyltransferase | Metallosphaera sedula | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
ATP + 4-hydroxybutanoate + CoA | - |
Metallosphaera sedula DSM 5348 | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
ATP + 4-hydroxybutanoate + CoA | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway. The flux between the succinate and acetyl-CoA branches in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway is governed by 4-hydroxybutyrate-CoA ligase, possibly regulated posttranslationally by the protein acetyltransferase | Metallosphaera sedula DSM 5348 | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-hydroxybutyrate-CoA ligase (AMP forming) | - |
Metallosphaera sedula |
Msed_0406 | locus name | Metallosphaera sedula |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Metallosphaera sedula |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
4-hydroxybutanoate | pH 7.5, 70°C | Metallosphaera sedula |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Metallosphaera sedula |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway. The flux between the succinate and acetyl-CoA branches in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway is governed by 4-hydroxybutyrate-CoA ligase, possibly regulated posttranslationally by the protein acetyltransferase | Metallosphaera sedula |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
4-hydroxybutanoate | pH 7.5, 70°C | Metallosphaera sedula |