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Literature summary for 6.2.1.38 extracted from

  • Ougham, H.; Taylor, D.; Trudgill, P.
    Camphor revisited: Involvement of a unique monooxygenase in metabolism of 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetic acid by Pseudomonas putida (1983), J. Bacteriol., 153, 140-152.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.38

Protein Variants

Protein Variants Comment Organism
additional information the ability to grow on (+)-camphor is transferred to a recipient strain, Pseudomonas putida PaW340, which is a streptomycin-resistant tryptophan auxotroph that is derived from Pseudomonas putida (arvilla) mt-2 Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate + ATP + CoA Pseudomonas putida
-
 AMP + diphosphate + [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA
-
 ?
[(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate + ATP + CoA Pseudomonas putida C1 / ATCC 17453
-
 AMP + diphosphate + [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
no activity in Pseudomonas putida strain PaW340
-
-
-
Pseudomonas putida
-
-
-
Pseudomonas putida C1 / ATCC 17453
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:(+)-camphor-grown cell inducible enzyme, no or poor activity in succinate- and octanoate-grown cells Pseudomonas putida
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate + ATP + CoA
-
 Pseudomonas putida AMP + diphosphate + [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA
-
 ?
[(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate + ATP + CoA
-
 Pseudomonas putida C1 / ATCC 17453 AMP + diphosphate + [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA
-
 ?

Synonyms

Synonyms Comment Organism
2-oxo-3-4,5,5-trimethylcyclopentenylacetyl CoA synthetase
-
 Pseudomonas putida
2-oxo-3-4,5,5-trimethylcyclopentenylacetyl coenzyme A synthetase
-
 Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Pseudomonas putida

Expression

Organism Comment Expression
Pseudomonas putida the enzyme is induced by cell growth on (+)-camphor as carbon source up

General Information

General Information Comment Organism
metabolism the enzyme catalyzes a step in the (+)-camphor catabolism. Metabolism of the first ring cleavage intermediate, 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetic acid, occurs through the sequential action of two inducible enzymes, the coenzyme A ester synthetase and an oxygenase, EC 1.14.13.160 Pseudomonas putida