BRENDA - Enzyme Database show
show all sequences of 6.2.1.33

Purification and properties of 4-halobenzoate-coenzyme A ligase from Pseudomonas sp. CBS3

Loeffler, F.; Mueller, R.; Lingens, F.; Biol. Chem. Hoppe-Seyler 373, 1001-1007 (1992)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
-
Pseudomonas sp.
Fe2+
-
Pseudomonas sp.
SDS
1 mM, irreversibe
Pseudomonas sp.
Zn2+
-
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
0.065
4-Chlorobenzoate
-
Pseudomonas sp.
0.11
0.19
CoA
-
Pseudomonas sp.
2.4
3.5
ATP
-
Pseudomonas sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
77% of the activation with Mg2+
Pseudomonas sp.
Fe2+
70% of the activation with Mg2+
Pseudomonas sp.
Mg2+
maximal initial rate of catalysis is achieved with an Mg2+:ATP ratio of 1:0.66 to 1:1.33; required
Pseudomonas sp.
Mn2+
87% of the activation with Mg2+
Pseudomonas sp.
Ni2+
11% of the activation with Mg2+
Pseudomonas sp.
Zn2+
23% of the activation with Mg2+
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57000
-
2 * 57000, SDS-PAGE
Pseudomonas sp.
86000
120000
gel filtration
Pseudomonas sp.
125000
-
native gradient PAGE
Pseudomonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
111.6
-
-
Pseudomonas sp.
Storage Stability
Storage Stability
Organism
-20°C, stable for months
Pseudomonas sp.
4°C, 0.1-2.5 mg of protein per ml, half-life: 21 days
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-Bromobenzoate + CoA + ATP
at 105% of the activity with 4-chlorobenzoate
778
Pseudomonas sp.
4-Bromobenzoyl-CoA + AMP + diphosphate
-
-
-
-
4-Chlorobenzoate + CoA + ADP
reaction with ADP at 7.5% of the reaction with ATP
778
Pseudomonas sp.
4-Chlorobenzoyl-CoA + AMP + phosphate
-
-
-
-
4-Chlorobenzoate + CoA + ATP
negligible activity with UTP, CTP, and GTP
778
Pseudomonas sp.
4-Chlorobenzoyl-CoA + AMP + diphosphate
-
-
-
-
4-Fluorobenzoate + CoA + ATP
at 36% of the activity with 4-chlorobenzoate
778
Pseudomonas sp.
4-Fluorobenzoyl-CoA + AMP + diphosphate
-
-
-
-
4-Iodobenzoate + CoA + ATP
at 90% of the activity with 4-chlorobenzoate
778
Pseudomonas sp.
4-Iodobenzoyl-CoA + AMP + diphosphate
-
-
-
-
4-Methoxybenzoate + CoA + ATP
at 9% of the activity with 4-chlorobenzoate
778
Pseudomonas sp.
4-Methoxybenzoyl-CoA + AMP + diphosphate
-
-
-
-
4-Methylbenzoate + CoA + ATP
at 22% of the activity with 4-chlorobenzoate
778
Pseudomonas sp.
4-Methylbenzoyl-CoA + AMP + diphosphate
-
-
-
-
4-Nitrobenzoate + CoA + ATP
at 7% of the activity with 4-chlorobenzoate
778
Pseudomonas sp.
4-Nitrobenzoyl-CoA + AMP + diphosphate
-
-
-
-
Benzoate + CoA + ATP
at 5% of the activity with 4-chlorobenzoate
778
Pseudomonas sp.
Benzoyl-CoA + AMP + diphosphate
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 57000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Pseudomonas sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4
-
0.1-2.5 mg of protein per ml, half-life: 21 days
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
-
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
-
Pseudomonas sp.
Fe2+
-
Pseudomonas sp.
SDS
1 mM, irreversibe
Pseudomonas sp.
Zn2+
-
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
0.065
4-Chlorobenzoate
-
Pseudomonas sp.
0.11
0.19
CoA
-
Pseudomonas sp.
2.4
3.5
ATP
-
Pseudomonas sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
77% of the activation with Mg2+
Pseudomonas sp.
Fe2+
70% of the activation with Mg2+
Pseudomonas sp.
Mg2+
maximal initial rate of catalysis is achieved with an Mg2+:ATP ratio of 1:0.66 to 1:1.33; required
Pseudomonas sp.
Mn2+
87% of the activation with Mg2+
Pseudomonas sp.
Ni2+
11% of the activation with Mg2+
Pseudomonas sp.
Zn2+
23% of the activation with Mg2+
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57000
-
2 * 57000, SDS-PAGE
Pseudomonas sp.
86000
120000
gel filtration
Pseudomonas sp.
125000
-
native gradient PAGE
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
111.6
-
-
Pseudomonas sp.
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stable for months
Pseudomonas sp.
4°C, 0.1-2.5 mg of protein per ml, half-life: 21 days
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-Bromobenzoate + CoA + ATP
at 105% of the activity with 4-chlorobenzoate
778
Pseudomonas sp.
4-Bromobenzoyl-CoA + AMP + diphosphate
-
-
-
-
4-Chlorobenzoate + CoA + ADP
reaction with ADP at 7.5% of the reaction with ATP
778
Pseudomonas sp.
4-Chlorobenzoyl-CoA + AMP + phosphate
-
-
-
-
4-Chlorobenzoate + CoA + ATP
negligible activity with UTP, CTP, and GTP
778
Pseudomonas sp.
4-Chlorobenzoyl-CoA + AMP + diphosphate
-
-
-
-
4-Fluorobenzoate + CoA + ATP
at 36% of the activity with 4-chlorobenzoate
778
Pseudomonas sp.
4-Fluorobenzoyl-CoA + AMP + diphosphate
-
-
-
-
4-Iodobenzoate + CoA + ATP
at 90% of the activity with 4-chlorobenzoate
778
Pseudomonas sp.
4-Iodobenzoyl-CoA + AMP + diphosphate
-
-
-
-
4-Methoxybenzoate + CoA + ATP
at 9% of the activity with 4-chlorobenzoate
778
Pseudomonas sp.
4-Methoxybenzoyl-CoA + AMP + diphosphate
-
-
-
-
4-Methylbenzoate + CoA + ATP
at 22% of the activity with 4-chlorobenzoate
778
Pseudomonas sp.
4-Methylbenzoyl-CoA + AMP + diphosphate
-
-
-
-
4-Nitrobenzoate + CoA + ATP
at 7% of the activity with 4-chlorobenzoate
778
Pseudomonas sp.
4-Nitrobenzoyl-CoA + AMP + diphosphate
-
-
-
-
Benzoate + CoA + ATP
at 5% of the activity with 4-chlorobenzoate
778
Pseudomonas sp.
Benzoyl-CoA + AMP + diphosphate
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 57000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Pseudomonas sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4
-
0.1-2.5 mg of protein per ml, half-life: 21 days
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
-
Pseudomonas sp.
Other publictions for EC 6.2.1.33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
702299
Wu
The mechanism of domain altern ...
Alcaligenes sp. AL3007
Biochemistry
48
4115-4125
2009
-
-
-
1
2
-
-
9
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
9
-
-
-
-
-
-
-
-
9
9
690987
Reger
Structural characterization of ...
Alcaligenes sp.
Biochemistry
47
8016-8025
2008
-
-
-
1
-
-
1
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
1
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
690988
Wu
Mechanism of 4-chlorobenzoate: ...
Alcaligenes sp.
Biochemistry
47
8026-8039
2008
-
-
1
1
25
-
3
1
-
1
-
1
-
1
-
-
1
1
-
-
-
-
3
1
1
-
-
-
1
-
-
1
1
-
-
-
-
1
1
1
25
-
-
3
1
1
-
1
-
1
-
-
-
1
-
-
-
-
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
661151
Gulick
Crystal structure of 4-chlorob ...
Alcaligenes sp.
Biochemistry
43
8670-8679
2004
-
-
-
1
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661288
Zhou
The purification and character ...
Arthrobacter sp., Arthrobacter sp. TM-1
Biodegradation
15
97-109
2004
-
-
-
-
-
-
-
3
-
-
2
-
-
5
-
-
1
-
-
-
1
-
9
1
1
1
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
2
-
-
-
-
1
-
-
1
-
9
1
1
1
-
-
1
1
-
1
-
-
-
-
-
-
776
Chang
Acyl-adenylate motif of the ac ...
Pseudomonas sp., Pseudomonas sp. CBS3
Biochemistry
36
15650-15659
1997
-
-
-
-
5
-
-
15
-
-
-
-
-
10
-
-
1
1
-
-
-
-
2
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
15
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
777
Chang
Determination of the chemical ...
Pseudomonas sp., Pseudomonas sp. CBS3
Biochemistry
35
13478-13484
1996
-
-
-
-
-
-
-
-
-
-
-
2
-
10
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
781
Dunaway-Mariano
On the origins and functions o ...
Pseudomonas sp.
Biodegradation
5
259-276
1994
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
778
Loeffler
Purification and properties of ...
Pseudomonas sp.
Biol. Chem. Hoppe-Seyler
373
1001-1007
1992
-
-
-
-
-
-
4
3
-
6
3
-
-
2
-
-
1
-
-
-
1
2
9
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
3
-
6
3
-
-
-
-
1
-
-
1
2
9
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
779
Loeffler
-
Characterization of 4-chlorobe ...
Pseudomonas sp.
DECHEMA Biotechnol. Conf. (Pt. A. Microbial Principles in Bioprocesses: Cell Culture Technology - Downstream Processing and Recovery)
5
25-28
1992
-
-
-
-
-
-
6
3
-
-
-
1
-
1
-
-
-
-
-
-
-
-
9
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
3
-
-
-
1
-
-
-
-
-
-
-
-
9
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
780
Chang
Isolation and characterization ...
Pseudomonas sp.
Biochemistry
31
5605-5610
1992
-
-
1
-
-
-
-
3
-
3
2
-
-
3
-
-
1
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
3
2
-
-
-
-
1
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-