BRENDA - Enzyme Database
show all sequences of 6.2.1.33

Acyl-adenylate motif of the acyl-adenylate/thioester-forming enzyme superfamily: a site-directed mutagenesis study with the Pseudomonas sp. strain CBS3 4-chlorobenzoate:coenzyme A ligase

Chang, K.H.; Xiang, H.; Dunaway-Mariano, D.; Biochemistry 36, 15650-15659 (1997)

Data extracted from this reference:

Engineering
Protein Variants
Commentary
Organism
E306Q
mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate
Pseudomonas sp.
G163I
mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate
Pseudomonas sp.
G166I
mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate
Pseudomonas sp.
K169M
mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate
Pseudomonas sp.
P168A
mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.014
-
4-Chlorobenzoate
wild-type
Pseudomonas sp.
0.02
-
4-Chlorobenzoate
mutant K169M
Pseudomonas sp.
0.062
-
CoA
mutant G163I
Pseudomonas sp.
0.073
-
4-Chlorobenzoate
mutant G166I
Pseudomonas sp.
0.076
-
CoA
mutant K169M
Pseudomonas sp.
0.085
-
CoA
wild-type
Pseudomonas sp.
0.105
-
4-Chlorobenzoate
mutant G163I
Pseudomonas sp.
0.108
-
CoA
mutant G166I
Pseudomonas sp.
0.168
-
CoA
mutant E306Q
Pseudomonas sp.
0.253
-
4-Chlorobenzoate
mutant E306Q
Pseudomonas sp.
0.308
-
ATP
wild-type
Pseudomonas sp.
0.321
-
ATP
mutant E306Q
Pseudomonas sp.
0.83
-
ATP
mutant K169M
Pseudomonas sp.
0.981
-
ATP
mutant G166I
Pseudomonas sp.
1.314
-
ATP
mutant G163I
Pseudomonas sp.
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas sp.
-
; wild-type and mutant enzymes G163I, G166I, P168A, K169M and E306Q
-
Pseudomonas sp. CBS3
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pseudomonas sp.
Reaction
Reaction
Commentary
Organism
Reaction ID
4-Chlorobenzoate + CoA + ATP = 4-chlorobenzoyl-CoA + AMP + diphosphate
two-step reaction consisting of the adenylation of 4-chlorobenzoate with adenosine 5'-triphosphate followed by acyl transfer from 4-chlorobenzoyl adenosine 5'-monophosphate diester
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-Chlorobenzoate + CoA + ATP
-
776
Pseudomonas sp.
4-Chlorobenzoyl-CoA + AMP + diphosphate
-
776
Pseudomonas sp.
-
4-Chlorobenzoate + CoA + ATP
-
776
Pseudomonas sp. CBS3
4-Chlorobenzoyl-CoA + AMP + diphosphate
-
776
Pseudomonas sp. CBS3
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.036
-
4-Chlorobenzoate
mutant G163I
Pseudomonas sp.
0.039
-
ATP
mutant G163I
Pseudomonas sp.
0.04
-
CoA
mutant G163I
Pseudomonas sp.
0.7
-
4-Chlorobenzoate
mutant E306Q
Pseudomonas sp.
0.8
-
ATP
mutant E306Q
Pseudomonas sp.
0.9
-
ATP
mutant E306Q
Pseudomonas sp.
2.3
-
4-Chlorobenzoate
mutant G166I
Pseudomonas sp.
2.7
-
CoA
mutant G166I
Pseudomonas sp.
3.3
-
ATP
mutant G166I
Pseudomonas sp.
9.4
-
ATP
mutant K169M
Pseudomonas sp.
9.8
-
4-Chlorobenzoate
mutant K169M
Pseudomonas sp.
16
-
ATP
mutant K169M
Pseudomonas sp.
34
-
4-Chlorobenzoate
wild-type
Pseudomonas sp.
39
-
CoA
wild-type
Pseudomonas sp.
54
-
ATP
wild-type
Pseudomonas sp.
Engineering (protein specific)
Protein Variants
Commentary
Organism
E306Q
mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate
Pseudomonas sp.
G163I
mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate
Pseudomonas sp.
G166I
mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate
Pseudomonas sp.
K169M
mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate
Pseudomonas sp.
P168A
mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.014
-
4-Chlorobenzoate
wild-type
Pseudomonas sp.
0.02
-
4-Chlorobenzoate
mutant K169M
Pseudomonas sp.
0.062
-
CoA
mutant G163I
Pseudomonas sp.
0.073
-
4-Chlorobenzoate
mutant G166I
Pseudomonas sp.
0.076
-
CoA
mutant K169M
Pseudomonas sp.
0.085
-
CoA
wild-type
Pseudomonas sp.
0.105
-
4-Chlorobenzoate
mutant G163I
Pseudomonas sp.
0.108
-
CoA
mutant G166I
Pseudomonas sp.
0.168
-
CoA
mutant E306Q
Pseudomonas sp.
0.253
-
4-Chlorobenzoate
mutant E306Q
Pseudomonas sp.
0.308
-
ATP
wild-type
Pseudomonas sp.
0.321
-
ATP
mutant E306Q
Pseudomonas sp.
0.83
-
ATP
mutant K169M
Pseudomonas sp.
0.981
-
ATP
mutant G166I
Pseudomonas sp.
1.314
-
ATP
mutant G163I
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-Chlorobenzoate + CoA + ATP
-
776
Pseudomonas sp.
4-Chlorobenzoyl-CoA + AMP + diphosphate
-
776
Pseudomonas sp.
-
4-Chlorobenzoate + CoA + ATP
-
776
Pseudomonas sp. CBS3
4-Chlorobenzoyl-CoA + AMP + diphosphate
-
776
Pseudomonas sp. CBS3
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.036
-
4-Chlorobenzoate
mutant G163I
Pseudomonas sp.
0.039
-
ATP
mutant G163I
Pseudomonas sp.
0.04
-
CoA
mutant G163I
Pseudomonas sp.
0.7
-
4-Chlorobenzoate
mutant E306Q
Pseudomonas sp.
0.8
-
ATP
mutant E306Q
Pseudomonas sp.
0.9
-
ATP
mutant E306Q
Pseudomonas sp.
2.3
-
4-Chlorobenzoate
mutant G166I
Pseudomonas sp.
2.7
-
CoA
mutant G166I
Pseudomonas sp.
3.3
-
ATP
mutant G166I
Pseudomonas sp.
9.4
-
ATP
mutant K169M
Pseudomonas sp.
9.8
-
4-Chlorobenzoate
mutant K169M
Pseudomonas sp.
16
-
ATP
mutant K169M
Pseudomonas sp.
34
-
4-Chlorobenzoate
wild-type
Pseudomonas sp.
39
-
CoA
wild-type
Pseudomonas sp.
54
-
ATP
wild-type
Pseudomonas sp.
Other publictions for EC 6.2.1.33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
702299
Wu
The mechanism of domain altern ...
Alcaligenes sp. AL3007
Biochemistry
48
4115-4125
2009
-
-
-
1
2
-
-
9
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
9
-
-
-
-
-
-
-
-
9
9
690987
Reger
Structural characterization of ...
Alcaligenes sp.
Biochemistry
47
8016-8025
2008
-
-
-
1
-
-
1
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
1
1
2
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
690988
Wu
Mechanism of 4-chlorobenzoate: ...
Alcaligenes sp.
Biochemistry
47
8026-8039
2008
-
-
1
1
25
-
3
1
-
1
-
1
-
1
-
-
1
1
-
-
-
-
3
1
2
1
-
-
-
1
-
-
1
1
-
-
-
-
1
1
1
25
-
-
3
1
1
-
1
-
1
-
-
-
1
-
-
-
-
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
661151
Gulick
Crystal structure of 4-chlorob ...
Alcaligenes sp.
Biochemistry
43
8670-8679
2004
-
-
-
1
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661288
Zhou
The purification and character ...
Arthrobacter sp., Arthrobacter sp. TM-1
Biodegradation
15
97-109
2004
-
-
-
-
-
-
-
3
-
-
2
-
-
5
-
-
1
-
-
-
1
-
9
1
1
1
1
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
2
-
-
-
-
1
-
-
1
-
9
1
1
1
-
-
1
1
-
1
-
-
-
-
-
-
776
Chang
Acyl-adenylate motif of the ac ...
Pseudomonas sp., Pseudomonas sp. CBS3
Biochemistry
36
15650-15659
1997
-
-
-
-
5
-
-
15
-
-
-
-
-
10
-
-
1
1
-
-
-
-
2
-
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
15
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
777
Chang
Determination of the chemical ...
Pseudomonas sp., Pseudomonas sp. CBS3
Biochemistry
35
13478-13484
1996
-
-
-
-
-
-
-
-
-
-
-
2
-
10
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
781
Dunaway-Mariano
On the origins and functions o ...
Pseudomonas sp.
Biodegradation
5
259-276
1994
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
778
Loeffler
Purification and properties of ...
Pseudomonas sp.
Biol. Chem. Hoppe-Seyler
373
1001-1007
1992
-
-
-
-
-
-
4
3
-
6
3
-
-
2
-
-
1
-
-
-
1
2
9
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
3
-
6
3
-
-
-
-
1
-
-
1
2
9
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
779
Loeffler
-
Characterization of 4-chlorobe ...
Pseudomonas sp.
DECHEMA Biotechnol. Conf. (Pt. A. Microbial Principles in Bioprocesses: Cell Culture Technology - Downstream Processing and Recovery)
5
25-28
1992
-
-
-
-
-
-
6
3
-
-
-
1
-
1
-
-
-
-
-
-
-
-
9
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
3
-
-
-
1
-
-
-
-
-
-
-
-
9
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
780
Chang
Isolation and characterization ...
Pseudomonas sp.
Biochemistry
31
5605-5610
1992
-
-
1
-
-
-
-
3
-
3
2
-
-
3
-
-
1
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
3
2
-
-
-
-
1
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-