BRENDA - Enzyme Database show
show all sequences of 6.2.1.33

The purification and characterisation of 4-chlorobenzoate:CoA ligase and 4-chlorobenzoyl CoA dehalogenase from Arthrobacter sp. strain TM-1

Zhou, L.; Marks, T.S.; Poh, R.P.; Smith, R.J.; Chowdhry, B.Z.; Smith, A.R.; Biodegradation 15, 97-109 (2004)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0035
-
4-Chlorobenzoate
25°C, pH 7.0
Arthrobacter sp.
0.0238
-
ATP
25°C, pH 7.0
Arthrobacter sp.
0.03
-
CoA
25°C, pH 7.0
Arthrobacter sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
2 * 56000, SDS-PAGE
Arthrobacter sp.
112000
-
gel filtration
Arthrobacter sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arthrobacter sp.
-
-
-
Arthrobacter sp. TM-1
-
-
-
Purification (Commentary)
Commentary
Organism
-
Arthrobacter sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.86
-
-
Arthrobacter sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-Bromobenzoate + CoA + ATP
93% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp.
4-Bromobenzoyl-CoA + AMP + diphosphate
-
-
-
?
4-Bromobenzoate + CoA + ATP
93% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp. TM-1
4-Bromobenzoyl-CoA + AMP + diphosphate
-
-
-
?
4-Chlorobenzoate + CoA + ATP
-
661288
Arthrobacter sp.
4-Chlorobenzoyl-CoA + AMP + diphosphate
-
-
-
?
4-Chlorobenzoate + CoA + ATP
-
661288
Arthrobacter sp. TM-1
4-Chlorobenzoyl-CoA + AMP + diphosphate
-
-
-
?
4-Fluorobenzoate + CoA + ATP
67% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp.
4-Fluorobenzoyl-CoA + AMP + diphosphate
-
-
-
?
4-Fluorobenzoate + CoA + ATP
67% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp. TM-1
4-Fluorobenzoyl-CoA + AMP + diphosphate
-
-
-
?
4-Iodobenzoate + CoA + ATP
73% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp.
4-Iodobenzoyl-CoA + AMP + diphosphate
-
-
-
?
4-Iodobenzoate + CoA + ATP
73% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp. TM-1
4-Iodobenzoyl-CoA + AMP + diphosphate
-
-
-
?
Benzoate + CoA + ATP
62% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp.
Benzoyl-CoA + AMP + diphosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 56000, SDS-PAGE
Arthrobacter sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
-
Arthrobacter sp.
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
10
40
10°C: about 80% of maximal activity, 40°C: about 50% of maximal activity
Arthrobacter sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Arthrobacter sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.8
8.2
pH 5.8: about 50% of maximal activity, pH 8.2: about 80% of maximal activity
Arthrobacter sp.
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Arthrobacter sp.
-
-
5.8
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0035
-
4-Chlorobenzoate
25°C, pH 7.0
Arthrobacter sp.
0.0238
-
ATP
25°C, pH 7.0
Arthrobacter sp.
0.03
-
CoA
25°C, pH 7.0
Arthrobacter sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
2 * 56000, SDS-PAGE
Arthrobacter sp.
112000
-
gel filtration
Arthrobacter sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Arthrobacter sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.86
-
-
Arthrobacter sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-Bromobenzoate + CoA + ATP
93% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp.
4-Bromobenzoyl-CoA + AMP + diphosphate
-
-
-
?
4-Bromobenzoate + CoA + ATP
93% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp. TM-1
4-Bromobenzoyl-CoA + AMP + diphosphate
-
-
-
?
4-Chlorobenzoate + CoA + ATP
-
661288
Arthrobacter sp.
4-Chlorobenzoyl-CoA + AMP + diphosphate
-
-
-
?
4-Chlorobenzoate + CoA + ATP
-
661288
Arthrobacter sp. TM-1
4-Chlorobenzoyl-CoA + AMP + diphosphate
-
-
-
?
4-Fluorobenzoate + CoA + ATP
67% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp.
4-Fluorobenzoyl-CoA + AMP + diphosphate
-
-
-
?
4-Fluorobenzoate + CoA + ATP
67% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp. TM-1
4-Fluorobenzoyl-CoA + AMP + diphosphate
-
-
-
?
4-Iodobenzoate + CoA + ATP
73% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp.
4-Iodobenzoyl-CoA + AMP + diphosphate
-
-
-
?
4-Iodobenzoate + CoA + ATP
73% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp. TM-1
4-Iodobenzoyl-CoA + AMP + diphosphate
-
-
-
?
Benzoate + CoA + ATP
62% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp.
Benzoyl-CoA + AMP + diphosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 56000, SDS-PAGE
Arthrobacter sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
-
Arthrobacter sp.
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
10
40
10°C: about 80% of maximal activity, 40°C: about 50% of maximal activity
Arthrobacter sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Arthrobacter sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.8
8.2
pH 5.8: about 50% of maximal activity, pH 8.2: about 80% of maximal activity
Arthrobacter sp.
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Arthrobacter sp.
-
-
5.8
Other publictions for EC 6.2.1.33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
702299
Wu
The mechanism of domain altern ...
Alcaligenes sp. AL3007
Biochemistry
48
4115-4125
2009
-
-
-
1
2
-
-
9
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
9
-
-
-
-
-
-
-
-
9
9
690987
Reger
Structural characterization of ...
Alcaligenes sp.
Biochemistry
47
8016-8025
2008
-
-
-
1
-
-
1
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
1
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
690988
Wu
Mechanism of 4-chlorobenzoate: ...
Alcaligenes sp.
Biochemistry
47
8026-8039
2008
-
-
1
1
25
-
3
1
-
1
-
1
-
1
-
-
1
1
-
-
-
-
3
1
1
-
-
-
1
-
-
1
1
-
-
-
-
1
1
1
25
-
-
3
1
1
-
1
-
1
-
-
-
1
-
-
-
-
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
661151
Gulick
Crystal structure of 4-chlorob ...
Alcaligenes sp.
Biochemistry
43
8670-8679
2004
-
-
-
1
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661288
Zhou
The purification and character ...
Arthrobacter sp., Arthrobacter sp. TM-1
Biodegradation
15
97-109
2004
-
-
-
-
-
-
-
3
-
-
2
-
-
5
-
-
1
-
-
-
1
-
9
1
1
1
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
2
-
-
-
-
1
-
-
1
-
9
1
1
1
-
-
1
1
-
1
-
-
-
-
-
-
776
Chang
Acyl-adenylate motif of the ac ...
Pseudomonas sp., Pseudomonas sp. CBS3
Biochemistry
36
15650-15659
1997
-
-
-
-
5
-
-
15
-
-
-
-
-
10
-
-
1
1
-
-
-
-
2
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
15
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
777
Chang
Determination of the chemical ...
Pseudomonas sp., Pseudomonas sp. CBS3
Biochemistry
35
13478-13484
1996
-
-
-
-
-
-
-
-
-
-
-
2
-
10
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
781
Dunaway-Mariano
On the origins and functions o ...
Pseudomonas sp.
Biodegradation
5
259-276
1994
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
778
Loeffler
Purification and properties of ...
Pseudomonas sp.
Biol. Chem. Hoppe-Seyler
373
1001-1007
1992
-
-
-
-
-
-
4
3
-
6
3
-
-
2
-
-
1
-
-
-
1
2
9
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
3
-
6
3
-
-
-
-
1
-
-
1
2
9
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
779
Loeffler
-
Characterization of 4-chlorobe ...
Pseudomonas sp.
DECHEMA Biotechnol. Conf. (Pt. A. Microbial Principles in Bioprocesses: Cell Culture Technology - Downstream Processing and Recovery)
5
25-28
1992
-
-
-
-
-
-
6
3
-
-
-
1
-
1
-
-
-
-
-
-
-
-
9
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
3
-
-
-
1
-
-
-
-
-
-
-
-
9
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
780
Chang
Isolation and characterization ...
Pseudomonas sp.
Biochemistry
31
5605-5610
1992
-
-
1
-
-
-
-
3
-
3
2
-
-
3
-
-
1
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
3
2
-
-
-
-
1
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-