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Literature summary for 6.2.1.30 extracted from

  • Imolorhe, I.A.; Cardona, S.T.
    3-Hydroxyphenylacetic acid induces the Burkholderia cenocepacia phenylacetic acid degradation pathway - toward understanding the contribution of aromatic catabolism to pathogenesis (2011), Front. Cell. Infect. Microbiol., 1, 14.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.30

Cloned(Commentary)

Cloned (Comment) Organism
gene paaK1 is encoded in the paaFZJGIK1 operon located on the chromosome 1 Burkholderia cenocepacia
gene paaK2 is encoded in the paaHK2paaG operon located on the chromosome 2 Burkholderia cenocepacia

Protein Variants

Protein Variants Comment Organism
additional information contruction of paak1 deletion mutant IAI1, insertional mutagenesis of paaK1, which encodes phenylacetate-CoA ligase, does not result in a phenylacetate-conditional growth Burkholderia cenocepacia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + phenylacetate + CoA Burkholderia cenocepacia
-
 AMP + diphosphate + phenylacetyl-CoA
-
 ?
ATP + phenylacetate + CoA Burkholderia cenocepacia DSM 16553
-
 AMP + diphosphate + phenylacetyl-CoA
-
 ?
additional information Burkholderia cenocepacia isozyme PaaK1 is more active than isozyme PaaK2, the larger binding pocket of PaaK1 can accommodate hydroxylated phenylacetate-derived molecules such as 3-hydroxyphenylacetic acid and 4-hydroxyphenylacetic acid, PaaK1 shows higher activity and broader substrate specificity than PaaK2 ?
-
 ?
additional information Burkholderia cenocepacia DSM 16553 isozyme PaaK1 is more active than isozyme PaaK2, the larger binding pocket of PaaK1 can accommodate hydroxylated phenylacetate-derived molecules such as 3-hydroxyphenylacetic acid and 4-hydroxyphenylacetic acid, PaaK1 shows higher activity and broader substrate specificity than PaaK2 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Burkholderia cenocepacia B4E7B5 gene paaK1
-
Burkholderia cenocepacia B4EL89 gene paaK2
-
Burkholderia cenocepacia DSM 16553 B4E7B5 gene paaK1
-
Burkholderia cenocepacia DSM 16553 B4EL89 gene paaK2
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information Burkholderia cenocepacia utilizes 3-hydroxyphenylacetic acid and 4-hydroxyphenylacetic acid as sole carbon sources, but not exclusively through phenylacetate degradation Burkholderia cenocepacia
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + phenylacetate + CoA
-
 Burkholderia cenocepacia AMP + diphosphate + phenylacetyl-CoA
-
 ?
ATP + phenylacetate + CoA
-
 Burkholderia cenocepacia DSM 16553 AMP + diphosphate + phenylacetyl-CoA
-
 ?
additional information isozyme PaaK1 is more active than isozyme PaaK2, the larger binding pocket of PaaK1 can accommodate hydroxylated phenylacetate-derived molecules such as 3-hydroxyphenylacetic acid and 4-hydroxyphenylacetic acid, PaaK1 shows higher activity and broader substrate specificity than PaaK2 Burkholderia cenocepacia ?
-
 ?
additional information isozyme PaaK1 is more active than isozyme PaaK2, the larger binding pocket of PaaK1 can accommodate hydroxylated phenylacetate-derived molecules such as 3-hydroxyphenylacetic acid and 4-hydroxyphenylacetic acid, PaaK1 shows higher activity and broader substrate specificity than PaaK2 Burkholderia cenocepacia DSM 16553 ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Burkholderia cenocepacia

Expression

Organism Comment Expression
Burkholderia cenocepacia 3-hydroxyphenylacetic acid induces the phenylacetic acid degradation pathway up
Burkholderia cenocepacia 3-hydroxyphenylacetic acid induces the phenylacetic acid degradation pathway, while 4-hydroxyphenylacetic acid does not up

General Information

General Information Comment Organism
additional information insertional mutagenesis of paaK1, which encodes phenylacetate-CoA ligase, does not result in a phenylacetate-conditional growth probably due to the presence of a putative paralogue gene paaK2 Burkholderia cenocepacia
additional information insertional mutagenesis of paaK1, which encodes phenylacetate-CoA ligase, does not result in a phenylacetate-conditional growth probably due to the presence of a putative paralogue gene paaK2. The paaK1 deletion mutant IAI1 does not show any growth-defective phenotype in phenylacetate Burkholderia cenocepacia
physiological function isozyme PaaK1 does not play a distinct role in pathogenesis of Burkholderia cenocepacia in Caenorhabditis elegans, although the catabolic pathway for phenylacetic acid degradation is a requirement for full pathogenesis Burkholderia cenocepacia