General Stability | Organism |
---|---|
enzyme is rather labile and requires the presence of glycerol and/or phenylacetate for stabilization | Pseudomonas sp. |
purified enzyme is sensitive to repeated freezing | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 2 mM, 87% inhibition | Pseudomonas sp. | |
iodoacetamide | - |
Pseudomonas sp. | |
Mo5+ | 2 mM, 80% inhibition | Pseudomonas sp. | |
NaF | - |
Pseudomonas sp. | |
NEM | - |
Pseudomonas sp. | |
Ni2+ | 1 mM, 65% inhibition | Pseudomonas sp. | |
p-hydroxymercuribenzoate | - |
Pseudomonas sp. | |
Zn2+ | 2 mM, 84% inhibition | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
phenylacetate | pH 7.8, 30°C | Pseudomonas sp. | |
0.15 | - |
CoA | pH 7.8, 30°C | Pseudomonas sp. | |
0.29 | - |
ATP | pH 7.8, 30°C | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Pseudomonas sp. | |
Mg2+ | optimal concentration: 5 mM | Pseudomonas sp. | |
Mn2+ | at 5 mM, 41% of the activation with Mg2+ | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
1 * 50000, SDS-PAGE | Pseudomonas sp. |
52000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + phenylacetate + CoA | Pseudomonas sp. | first step in anaerobic degradation pathway of phenylacetate | ? | - |
? | |
ATP + phenylacetate + CoA | Pseudomonas sp. KB 740 | first step in anaerobic degradation pathway of phenylacetate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. KB 740 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
insensitive to oxygen | Pseudomonas sp. |
Purification (Comment) | Organism |
---|---|
strain KB 740 | Pseudomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | maximal activity in mid-exponential growth pase | Pseudomonas sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24.4 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C or -70°C, 70% loss of activity after 1 week | Pseudomonas sp. |
4°C, 90% loss of activity after 1 day without glycerol, plus 10% glycerol, half-life: 2 days, plus 5 mM phenylacetate, half-life: 1 week, plus 35% glycerol, half-life: 2 weeks | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + phenylacetate + CoA | highly specific | Pseudomonas sp. | AMP + diphosphate + phenylacetyl-CoA | - |
? | |
ATP + phenylacetate + CoA | highly specific | Pseudomonas sp. KB 740 | AMP + diphosphate + phenylacetyl-CoA | - |
? | |
ATP + phenylacetate + CoA | first step in anaerobic degradation pathway of phenylacetate | Pseudomonas sp. | ? | - |
? | |
ATP + phenylacetate + CoA | first step in anaerobic degradation pathway of phenylacetate | Pseudomonas sp. KB 740 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.7 | - |
ATP | pH 7.8, 30°C | Pseudomonas sp. | |
21.7 | - |
CoA | pH 7.8, 30°C | Pseudomonas sp. | |
21.7 | - |
phenylacetate | pH 7.8, 30°C | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | pH 8.5: activity maximum, pH 9.0: 50% of maximal activity | Pseudomonas sp. |