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Literature summary for 6.2.1.27 extracted from

  • Laempe, D.; Jahn, M.; Breese, K.; Schägger, H.; Fuchs, G.
    Anaerobic metabolism of 3-hydroxybenzoate by the denitrifying bacterium Thauera aromatica (2001), J. Bacteriol., 183, 968-979.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Thauera aromatica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.065
-
4-hydroxybenzoate pH 7.8, 30°C Thauera aromatica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
gel filtration Thauera aromatica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-hydroxybenzoate + CoA Thauera aromatica the enzyme is involved in the anaerobic hydroxybenzoate metabolism AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Thauera aromatica Q9AJS8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thauera aromatica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.12
-
pH 7.8, 30°C Thauera aromatica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-hydroxybenzoate + CoA the enzyme is involved in the anaerobic hydroxybenzoate metabolism Thauera aromatica AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
?
ATP + 4-hydroxybenzoate + CoA the bifunctional enzyme is also active with 3-hydroxybenzoate (cf. EC 6.2.1.37). Activity with 4-hydroxybenzoate is 83% of the activity with 3-hydroxybenzoate Thauera aromatica AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
?
additional information the enzyme shows low activity towards benzoate, 4-aminobenzoate, 3-aminobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-chlorobenzoate, and 4-chlorobenzoate. There is no activity with 3,4-dihydroxybenzoate, 2,3-dihydroxybenzoate, and 2-hydroxybenzoate as substrates Thauera aromatica ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE Thauera aromatica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
or above. At a pH of above 9 the coupled spectrophotometric assay becomes limited due to instability of the substrates Thauera aromatica

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7.0: 20% of the activity at pH 9, pH 8.0: 65% of the activity at pH 9 Thauera aromatica

Expression

Organism Comment Expression
Thauera aromatica induction during anaerobic growth with by 3-hydroxybenzoate up