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Literature summary for 6.2.1.27 extracted from

  • Biegert, T.; Altenschmidt, U.; Eckerskorn, C.; Fuchs, G.
    Enzymes of anarobic metabolism pf phenolic compounds. 4-Hydroxybenzoate-CoA ligase from a denitrifying Pseudomonas species (1993), Eur. J. Biochem., 213, 555-561.
    View publication on PubMed
Search for more literature for 6.2.1.27

Inhibitors

Inhibitors Comment Organism Structure
2,4-Dihydroxybenzoate strong Pseudomonas sp.
2-hydroxy-4-methylbenzoate strong Pseudomonas sp.
5,5'-dithiobis(2-nitrobenzoate)
-
 Pseudomonas sp.
PCMB
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
 4-hydroxybenzoate pH 7.8, 37°C Pseudomonas sp.
0.077
-
 ATP pH 7.8, 37°C Pseudomonas sp.
0.125
-
 coenzyme A pH 7.8, 37°C Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-hydroxybenzoate + CoA Pseudomonas sp. initial step of anaerobic 4-hydroxybenzoate degradation AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
ATP + 4-hydroxybenzoate + CoA Pseudomonas sp. K172 initial step of anaerobic 4-hydroxybenzoate degradation AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. K172
-
-
-

Oxidation Stability

Oxidation Stability Organism
oxygen-sensitive during initial stages of purification, can be stabilized by adding DTT and 10% glycerol Pseudomonas sp.

Purification (Commentary)

Purification (Comment) Organism
strain K172 Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:4-hydroxybenzoate-grown cell in the mid-exponential growth phase Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
37.3
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-aminobenzoate + CoA 30% of the activity with 4-hydroxybenzoate Pseudomonas sp. AMP + 4-aminobenzoyl-CoA + diphosphate
-
 ?
ATP + 4-fluorobenzoate + CoA 3.8% of the activity with 4-hydroxybenzoate Pseudomonas sp. AMP + 4-fluorobenzoyl-CoA + diphosphate
-
 ?
ATP + 4-hydroxybenzoate + CoA highly specific Pseudomonas sp. AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
ATP + 4-hydroxybenzoate + CoA anaerob Pseudomonas sp. AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
ATP + 4-hydroxybenzoate + CoA initial step of anaerobic 4-hydroxybenzoate degradation Pseudomonas sp. AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
ATP + 4-hydroxybenzoate + CoA highly specific Pseudomonas sp. K172 AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
ATP + 4-hydroxybenzoate + CoA anaerob Pseudomonas sp. K172 AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
ATP + 4-hydroxybenzoate + CoA initial step of anaerobic 4-hydroxybenzoate degradation Pseudomonas sp. K172 AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
ATP + phenylacetate + CoA 4.5% of the activity with 4-hydroxybenzoate Pseudomonas sp. AMP + phenylacetyl-CoA + diphosphate
-
 ?
CTP + 4-hydroxybenzoate + CoA 60% of the activity with ATP Pseudomonas sp. CMP + 4-hydroxybenzoyl-CoA + diphosphate
-
 ?
CTP + 4-hydroxybenzoate + CoA 60% of the activity with ATP Pseudomonas sp. K172 CMP + 4-hydroxybenzoyl-CoA + diphosphate
-
 ?
additional information not: benzoate, 3- or 2-hydroxybenzoate Pseudomonas sp. ?
-
 ?
additional information not: benzoate, 3- or 2-hydroxybenzoate Pseudomonas sp. K172 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 48000, SDS-PAGE Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
30
-
 4-hydroxybenzoate pH 7.8, 37°C Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2 8.5
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 50% of activity maximum at pH 7.0 and 9.5, almost no activity at pH 6.0 and 10.0 Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0012
-
 2,4-Dihydroxybenzoate pH 7.8, 37°C Pseudomonas sp.
0.0014
-
 2-hydroxy-4-methylbenzoate pH 7.8, 37°C Pseudomonas sp.