Protein Variants | Comment | Organism |
---|---|---|
H341A | mutant enzyme loses 65% of ist activity and the Km-value for ATP increases 5.4fold | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethylpyrocarbonate | inactivation follows pseudo-first-order kinetics with a second-order rate constant of 0.00092 /min *microM, partial protection from inactivation by either o-succinylbenzoic acid, ATP, or ATP plus Mg2+ while inactivation is completely prevented by the presence of the combination of ATP, Mg2+ and o-succinylbenzoate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0735 | - |
ATP | native enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-succinylbenzoate + CoA | - |
Escherichia coli | AMP + diphosphate + 2-succinylbenzoyl-CoA | - |
? | |
additional information | His341 plays an important role in catalysis since it is probably involved in the binding of ATP to the enzyme | Escherichia coli | ? | - |
? |