Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 Star (DE3) | Paraburkholderia xenovorans |
Crystallization (Comment) | Organism |
---|---|
sitting-drop vapor diffusion method at 18 °C in 23% PEG 3350 K and 0.2 M sodium formate | Paraburkholderia xenovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
benzoate | in Tris-HCl at pH 8.0 | Paraburkholderia xenovorans | |
0.18 | - |
CoA | in Tris-HCl at pH 8.0 | Paraburkholderia xenovorans | |
0.25 | - |
2-Aminobenzoate | in Tris-HCl at pH 8.0 | Paraburkholderia xenovorans | |
0.27 | - |
ATP | in Tris-HCl at pH 8.0 | Paraburkholderia xenovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paraburkholderia xenovorans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Paraburkholderia xenovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-aminobenzoate + CoA | 12.7% activity compared to benzoate | Paraburkholderia xenovorans | AMP + diphosphate + 2-aminobenzoyl-CoA | - |
? | |
ATP + 2-fluorobenzoate + CoA | 1.9% activity compared to benzoate | Paraburkholderia xenovorans | AMP + diphosphate + 2-fluorobenzoyl-CoA | - |
? | |
ATP + 3-fluorobenzoate + CoA | 2.0% activity compared to benzoate | Paraburkholderia xenovorans | AMP + diphosphate + 3-fluorobenzoyl-CoA | - |
? | |
ATP + 3-hydroxybenzoate + CoA | 1.1% activity compared to benzoate | Paraburkholderia xenovorans | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? | |
ATP + 4-fluorobenzoate + CoA | 1.4% activity compared to benzoate | Paraburkholderia xenovorans | AMP + diphosphate + 4-fluorobenzoyl-CoA | - |
? | |
ATP + 4-hydroxybenzoate + CoA | 1.0% activity compared to benzoate | Paraburkholderia xenovorans | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
ATP + benzoate + CoA | 100% activity | Paraburkholderia xenovorans | AMP + diphosphate + benzoyl-CoA | - |
? | |
additional information | no or less than 1% activity with 2-methylbenzoate, 4-methylbenzoate, 3-chlorobenzoate, 4-chlorobenzoate, 2-bromobenzoate, 3-bromobenzoate, 4-bromobenzoate, phenylacetate, acetate, succinate, propionate, butyrate, and hexanoate | Paraburkholderia xenovorans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BCLM | - |
Paraburkholderia xenovorans |
benzoate CoA ligase | - |
Paraburkholderia xenovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Paraburkholderia xenovorans |