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show all sequences of 6.2.1.22

Copurification of citrate lyase and citrate lyase ligase from Rhodopseudomonas gelatinosa and subsequent separation of the two enzymes

Antranikian, G.; Gottschalk, G.; Eur. J. Biochem. 126, 43-47 (1982)

Data extracted from this reference:

General Stability
General Stability
Organism
stabilized by nucleotides, most potent stabilizer is ADP
Rubrivivax gelatinosus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.000037
-
citrate lyase
sulfhydryl form
Rubrivivax gelatinosus
0.14
-
ATP
-
Rubrivivax gelatinosus
5
-
acetate
-
Rubrivivax gelatinosus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required, optimal concentration: 3 mM, inhibitory above 5 mM
Rubrivivax gelatinosus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37500
-
gel filtration, density gradient centrifugation
Rubrivivax gelatinosus
41690
-
1 * 41690, SDS-PAGE
Rubrivivax gelatinosus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rubrivivax gelatinosus
-
-
-
Purification (Commentary)
Commentary
Organism
copurification of citrate lyase and citrate lyase ligase
Rubrivivax gelatinosus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Rubrivivax gelatinosus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + acetate + citrate (pro-3S)-lyase
thiol form of (pro-3S)-lyase
697
Rubrivivax gelatinosus
AMP + diphosphate + citrate(pro-3S)-lyase
-
-
-
-
ATP + acetate + citrate (pro-3S)-lyase
highly specific, cannot react with citrate lyases from nonphototrophic microorganisms, e.g. Enterobacter aerogenes, Clostridium sphenoides or Streptococcus diacetilactis
697
Rubrivivax gelatinosus
AMP + diphosphate + citrate(pro-3S)-lyase
-
-
-
-
ATP + propionate + citrate (pro-3S)-lyase
thiol form of (pro-3S)-lyase, propionate at 47% of the activity relative to acetate
697
Rubrivivax gelatinosus
AMP + diphosphate + citrate(pro-3S)-lyase
-
-
-
-
CTP + acetate + citrate (pro-3S)-lyase
thiol form of (pro-3S)-lyase, CTP at 30% of the relative to ATP
697
Rubrivivax gelatinosus
CMP + diphosphate + citrate(pro-3S)-lyase
-
-
-
-
dTTP + acetate + citrate (pro-3S)-lyase
thiol form of (pro-3S)-lyase, dTTP at 5% of the activity relative to ATP
697
Rubrivivax gelatinosus
dTMP + diphosphate + citrate(pro-3S)-lyase
-
-
-
-
GTP + acetate + citrate (pro-3S)-lyase
thiol form of (pro-3S)-lyase, GTP at 43% of the activity of ATP
697
Rubrivivax gelatinosus
GMP + diphosphate + citrate(pro-3S)-lyase
-
-
-
-
ITP + acetate + citrate (pro-3S)-lyase
thiol form of (pro-3S)-lyase, ITP at 46% of the activity relative to ATP
697
Rubrivivax gelatinosus
IMP + diphosphate + citrate(pro-3S)-lyase
-
-
-
-
UTP + acetate + citrate (pro-3S)-lyase
thiol form of (pro-3S)-lyase, UTP at 18% of the activity relative to ATP
697
Rubrivivax gelatinosus
UMP + diphosphate + citrate(pro-3S)-lyase
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 41690, SDS-PAGE
Rubrivivax gelatinosus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
-
Rubrivivax gelatinosus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
8
6: 50% of maximal activity, 8: 55% of maximal activity
Rubrivivax gelatinosus
General Stability (protein specific)
General Stability
Organism
stabilized by nucleotides, most potent stabilizer is ADP
Rubrivivax gelatinosus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.000037
-
citrate lyase
sulfhydryl form
Rubrivivax gelatinosus
0.14
-
ATP
-
Rubrivivax gelatinosus
5
-
acetate
-
Rubrivivax gelatinosus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required, optimal concentration: 3 mM, inhibitory above 5 mM
Rubrivivax gelatinosus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37500
-
gel filtration, density gradient centrifugation
Rubrivivax gelatinosus
41690
-
1 * 41690, SDS-PAGE
Rubrivivax gelatinosus
Purification (Commentary) (protein specific)
Commentary
Organism
copurification of citrate lyase and citrate lyase ligase
Rubrivivax gelatinosus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Rubrivivax gelatinosus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + acetate + citrate (pro-3S)-lyase
thiol form of (pro-3S)-lyase
697
Rubrivivax gelatinosus
AMP + diphosphate + citrate(pro-3S)-lyase
-
-
-
-
ATP + acetate + citrate (pro-3S)-lyase
highly specific, cannot react with citrate lyases from nonphototrophic microorganisms, e.g. Enterobacter aerogenes, Clostridium sphenoides or Streptococcus diacetilactis
697
Rubrivivax gelatinosus
AMP + diphosphate + citrate(pro-3S)-lyase
-
-
-
-
ATP + propionate + citrate (pro-3S)-lyase
thiol form of (pro-3S)-lyase, propionate at 47% of the activity relative to acetate
697
Rubrivivax gelatinosus
AMP + diphosphate + citrate(pro-3S)-lyase
-
-
-
-
CTP + acetate + citrate (pro-3S)-lyase
thiol form of (pro-3S)-lyase, CTP at 30% of the relative to ATP
697
Rubrivivax gelatinosus
CMP + diphosphate + citrate(pro-3S)-lyase
-
-
-
-
dTTP + acetate + citrate (pro-3S)-lyase
thiol form of (pro-3S)-lyase, dTTP at 5% of the activity relative to ATP
697
Rubrivivax gelatinosus
dTMP + diphosphate + citrate(pro-3S)-lyase
-
-
-
-
GTP + acetate + citrate (pro-3S)-lyase
thiol form of (pro-3S)-lyase, GTP at 43% of the activity of ATP
697
Rubrivivax gelatinosus
GMP + diphosphate + citrate(pro-3S)-lyase
-
-
-
-
ITP + acetate + citrate (pro-3S)-lyase
thiol form of (pro-3S)-lyase, ITP at 46% of the activity relative to ATP
697
Rubrivivax gelatinosus
IMP + diphosphate + citrate(pro-3S)-lyase
-
-
-
-
UTP + acetate + citrate (pro-3S)-lyase
thiol form of (pro-3S)-lyase, UTP at 18% of the activity relative to ATP
697
Rubrivivax gelatinosus
UMP + diphosphate + citrate(pro-3S)-lyase
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 41690, SDS-PAGE
Rubrivivax gelatinosus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
-
Rubrivivax gelatinosus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
8
6: 50% of maximal activity, 8: 55% of maximal activity
Rubrivivax gelatinosus
Other publictions for EC 6.2.1.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
651344
Martin
Cloning and molecular characte ...
Weissella paramesenteroides
FEMS Microbiol. Lett.
174
231-238
1999
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3
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1
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1
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33346
Bekal
Purification of Leuconostoc me ...
Leuconostoc mesenteroides
J. Bacteriol.
180
647-654
1998
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1
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7
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1
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1
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1
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1
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1
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694
Antranikian
Phosphorylation of citrate lya ...
Clostridium sphenoides
Biochimie
71
1029-1037
1989
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2
-
1
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4
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1
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2
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1
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1
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695
Antranikian
Covalent modification of citra ...
Clostridium sphenoides, Clostridium sphenoides C2 / DSM 614, Clostridium sporosphaeroides, Lactococcus lactis
Eur. J. Biochem.
153
413-420
1985
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-
-
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-
1
1
-
2
2
2
-
8
-
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1
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1
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10
1
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1
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1
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1
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2
2
2
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1
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1
-
10
1
-
-
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-
1
-
-
-
-
-
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-
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-
698
Quentmeier
Characterization of citrate ly ...
Clostridium sporosphaeroides
Arch. Microbiol.
141
85-90
1985
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4
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1
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1
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-
-
-
-
-
-
-
697
Antranikian
Copurification of citrate lyas ...
Rubrivivax gelatinosus
Eur. J. Biochem.
126
43-47
1982
-
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-
-
-
1
-
3
-
1
2
-
-
4
-
-
1
-
-
-
1
-
8
1
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-
-
1
1
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-
1
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3
-
1
2
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1
-
-
1
-
8
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
700
Antranikian
Activation and inactivation of ...
Rubrivivax gelatinosus
FEBS Lett.
88
67-70
1978
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2
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1
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693
Kummel
Citrate lyase from Streptococc ...
Lactococcus lactis subsp. lactis, Leuconostoc citrovorum
Arch. Microbiol.
102
111-116
1975
-
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1
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2
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1
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4
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1
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1
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4
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696
Schmellenkamp
Mechanism of enzymic acetylati ...
Klebsiella aerogenes
Proc. Natl. Acad. Sci. USA
71
1987-1991
1974
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3
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1
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3
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1
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1
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