KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADPH | pH 7.8, 55°C | Chloroflexus aurantiacus | |
0.01 | - |
CoA | pH 7.8, 55°C | Chloroflexus aurantiacus | |
0.015 | - |
3-hydroxypropionate | pH 7.8, 55°C | Chloroflexus aurantiacus | |
0.05 | - |
ATP | pH 7.8, 55°C | Chloroflexus aurantiacus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cs+ | can partially substitute for K+ | Chloroflexus aurantiacus | |
K+ | required, half aximal activity at 4 mM | Chloroflexus aurantiacus | |
Mg2+ | required | Chloroflexus aurantiacus | |
NH4+ | can partially substitute for K+ | Chloroflexus aurantiacus | |
Rb+ | can partially substitute for K+ | Chloroflexus aurantiacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
185000 | - |
SDS-PAGE | Chloroflexus aurantiacus |
500000 | 800000 | gel filtration | Chloroflexus aurantiacus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-hydroxypropionate + CoA + NADPH | Chloroflexus aurantiacus | involved in CO2 fixation via 3-hydroxypropionate cycle | AMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
ATP + 3-hydroxypropionate + CoA + NADPH | Chloroflexus aurantiacus OK-70-fl / DSM 636 | involved in CO2 fixation via 3-hydroxypropionate cycle | AMP + diphosphate + NADP+ + propionyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chloroflexus aurantiacus | Q8VRG6 | - |
- |
Chloroflexus aurantiacus OK-70-fl / DSM 636 | Q8VRG6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Chloroflexus aurantiacus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.5 | - |
purified enzyme, pH 7.8, 55°C | Chloroflexus aurantiacus |
Storage Stability | Organism |
---|---|
-20°C, 20% glycerol, 1 mM dithioerythreitol, stable for several weeks | Chloroflexus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-hydroxypropionate + CoA + NADPH | involved in CO2 fixation via 3-hydroxypropionate cycle | Chloroflexus aurantiacus | AMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
ATP + 3-hydroxypropionate + CoA + NADPH | three-step reaction with 3-hydroxypropionyl-CoA and acryly-CoA as intermediates | Chloroflexus aurantiacus | AMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
ATP + 3-hydroxypropionate + CoA + NADPH | involved in CO2 fixation via 3-hydroxypropionate cycle | Chloroflexus aurantiacus OK-70-fl / DSM 636 | AMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
ATP + 3-hydroxypropionate + CoA + NADPH | three-step reaction with 3-hydroxypropionyl-CoA and acryly-CoA as intermediates | Chloroflexus aurantiacus OK-70-fl / DSM 636 | AMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
ATP + acrylate + CoA + NADPH | same activity as with 3-hydroxypropionate | Chloroflexus aurantiacus | AMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
ATP + acrylate + CoA + NADPH | same activity as with 3-hydroxypropionate | Chloroflexus aurantiacus OK-70-fl / DSM 636 | AMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
GTP + 3-hydroxypropionate + CoA + NADPH | 24% of activity | Chloroflexus aurantiacus | GMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
GTP + 3-hydroxypropionate + CoA + NADPH | 24% of activity | Chloroflexus aurantiacus OK-70-fl / DSM 636 | GMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
additional information | no activity with glycolate, malonate, beta-alanine, 3-hydroxybutyrate, or crotonate | Chloroflexus aurantiacus | ? | - |
? | |
additional information | no activity with glycolate, malonate, beta-alanine, 3-hydroxybutyrate, or crotonate | Chloroflexus aurantiacus OK-70-fl / DSM 636 | ? | - |
? | |
UTP + 3-hydroxypropionate + CoA + NADPH | 20% of activity | Chloroflexus aurantiacus | UMP + diphosphate + NADP+ + propionyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | homotrimer or homotetramer | Chloroflexus aurantiacus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
stable for at least 15 min in presence of 20% glycerol and 1 mM dithioerythreitol | Chloroflexus aurantiacus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
3-hydroxypropionate | pH 7.8, 55°C | Chloroflexus aurantiacus |