Cloned (Comment) | Organism |
---|---|
gene Aacs, cloning and recombinant expression of FLAG-tagged enzyme in Lenti-X-293T cells. AACS and legumain are transiently expressed in HEK-293 cells | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetoacetate + CoA | Mus musculus | - |
AMP + diphosphate + acetoacetyl-CoA | - |
? | |
ATP + acetoacetate + CoA | Mus musculus ddY | - |
AMP + diphosphate + acetoacetyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9D2R0 | - |
- |
Mus musculus ddY | Q9D2R0 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | enzyme AACS is posttranslationally regulated, being cleaved at a specific site in the kidney. In vivo cleavage of enzyme AACS by legumain in HEK 293 cells generates the 55 kDa product from AACS. Incubation of recombinant AACS with recombinant legumain results in the degradation of AACS, optimally at pH 4.5. Knockdown of legumain with short-hairpin RNA against legumain using the hydrodynamics method leads to a decrease in the 55 kDa band of AACS in mouse kidney. Legumain is involved in the processing of AACS through the lysosomal degradation pathway in the kidney. Suppression of legumain results in a decrease in the cleaved form of AACS protein, and an increase in the full-length form of AACS protein. Legumain is involved in the cleavage of AACS in the kidney, suggesting that AACS is degraded by the lysosomal pathway | Mus musculus |
Purification (Comment) | Organism |
---|---|
recombinant FLAG-tagged enzyme from Lenti-X-293T cells by affinity chromatography and ultrafiltration | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipocyte | - |
Mus musculus | - |
kidney | the short form of an AACS band is detected in the kidney | Mus musculus | - |
liver | - |
Mus musculus | - |
additional information | no expression in cerebrum, cerebellum, skeletal muscle, spleen, heart, and lung, expression analysis, overview | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetoacetate + CoA | - |
Mus musculus | AMP + diphosphate + acetoacetyl-CoA | - |
? | |
ATP + acetoacetate + CoA | - |
Mus musculus ddY | AMP + diphosphate + acetoacetyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AACS | - |
Mus musculus |
Acetoacetyl-CoA synthetase | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
metabolism | Legumain is involved in the cleavage of AACS in the kidney, suggesting that AACS is degraded by the lysosomal pathway | Mus musculus |
physiological function | in the cytosol, acetoacetate is converted to acetoacetyl-CoA by acetoacetyl-CoA synthetase (AACS) for the synthesis of cholesterol and fatty acids. Acetoacetyl-CoA synthetase is a ketone body-utilizing enzyme, which is responsible for the synthesis of cholesterol and fatty acids from ketone bodies in lipogenic tissues, such as the liver and adipocytes. Enzyme AACS is posttranslationally regulated, being cleaved at a specific site in the kidney | Mus musculus |