General Stability | Organism |
---|---|
40% loss of activity on freezing and thawing without glycerol | Rattus norvegicus |
in presence of 5% glycerol the enzyme can be frozen and thawed five times without loss of activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | 5 mM, 70% inhibition | Rattus norvegicus | |
diphosphate | 10 mM, 70% inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
acetoacetate | - |
Rattus norvegicus | |
0.01 | - |
CoA | - |
Rattus norvegicus | |
0.06 | - |
ATP | - |
Rattus norvegicus | |
0.075 | - |
L-(+)-3-hydroxybutyrate | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cs+ | can replace K+ in activation | Rattus norvegicus | |
K+ | monovalent cation required | Rattus norvegicus | |
Mg2+ | required | Rattus norvegicus | |
Mn2+ | can replace Mg2+ in activation | Rattus norvegicus | |
additional information | absolute requirement for a monovalent cation and a divalent cation | Rattus norvegicus | |
NH4+ | can replace K+ in activation | Rattus norvegicus | |
Ni2+ | can replace Mg2+ in activation | Rattus norvegicus | |
Rb+ | can replace K+ in activation | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
1 * 71000, SDS-PAGE | Rattus norvegicus |
80000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.3 | - |
- |
Rattus norvegicus |
2.63 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, 0.1 mg/ml enzyme, 10 mM Tris-HCl, pH 7.5, 50% glycerol, 10 mM 2-mercaptoethanol, stable for at least 3 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetoacetate + CoA | no activity with GTP, UTP, CTP or ADP | Rattus norvegicus | AMP + diphosphate + acetoacetyl-CoA | - |
? | |
ATP + L-3-hydroxybutanoate + CoA | 48% of the activity relative to acetoacetate | Rattus norvegicus | AMP + diphosphate + L-3-hydroxybutyryl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 71000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.4 | - |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 8.6 | about 65% of maximal activity at pH 6.2 and 8.6 | Rattus norvegicus |