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Literature summary for 6.2.1.16 extracted from

  • Ito, M.; Fukui, T.; Kamokari, M.; Saito, T.; Tomita, K.
    Purification and characterization of acetoacetyl-CoA synthetase from rat liver (1984), Biochim. Biophys. Acta, 794, 183-193.
    View publication on PubMed

General Stability

General Stability Organism
40% loss of activity on freezing and thawing without glycerol Rattus norvegicus
in presence of 5% glycerol the enzyme can be frozen and thawed five times without loss of activity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
AMP 5 mM, 70% inhibition Rattus norvegicus
diphosphate 10 mM, 70% inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
acetoacetate
-
Rattus norvegicus
0.01
-
CoA
-
Rattus norvegicus
0.06
-
ATP
-
Rattus norvegicus
0.075
-
L-(+)-3-hydroxybutyrate
-
Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ can replace K+ in activation Rattus norvegicus
K+ monovalent cation required Rattus norvegicus
Mg2+ required Rattus norvegicus
Mn2+ can replace Mg2+ in activation Rattus norvegicus
additional information absolute requirement for a monovalent cation and a divalent cation Rattus norvegicus
NH4+ can replace K+ in activation Rattus norvegicus
Ni2+ can replace Mg2+ in activation Rattus norvegicus
Rb+ can replace K+ in activation Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
1 * 71000, SDS-PAGE Rattus norvegicus
80000
-
gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3
-
-
Rattus norvegicus
2.63
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, 0.1 mg/ml enzyme, 10 mM Tris-HCl, pH 7.5, 50% glycerol, 10 mM 2-mercaptoethanol, stable for at least 3 months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetoacetate + CoA no activity with GTP, UTP, CTP or ADP Rattus norvegicus AMP + diphosphate + acetoacetyl-CoA
-
?
ATP + L-3-hydroxybutanoate + CoA 48% of the activity relative to acetoacetate Rattus norvegicus AMP + diphosphate + L-3-hydroxybutyryl-CoA
-
?

Subunits

Subunits Comment Organism
monomer 1 * 71000, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.4
-
Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6.2 8.6 about 65% of maximal activity at pH 6.2 and 8.6 Rattus norvegicus