Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells | Thermococcus kodakarensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0056 | - |
CoA | at pH 6.5 and 55°C | Thermococcus kodakarensis | |
0.0556 | - |
ATP | at pH 6.5 and 55°C | Thermococcus kodakarensis | |
0.132 | - |
Isobutyrate | at pH 6.5 and 55°C | Thermococcus kodakarensis | |
0.322 | - |
thioglycolate | at pH 6.5 and 55°C | Thermococcus kodakarensis | |
0.329 | - |
phenylacetate | at pH 6.5 and 55°C | Thermococcus kodakarensis | |
1.5 | - |
acetate | at pH 6.5 and 55°C | Thermococcus kodakarensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | Thermococcus kodakarensis | - |
ADP + phosphate + acetyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | Q5JIA8 and Q5JIA9 | - |
- |
Purification (Comment) | Organism |
---|---|
Resource Q column chromatography and Superdex 200 gel filtration | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-methylbutyrate + CoA | - |
Thermococcus kodakarensis | ADP + phosphate + 2-methylbutyryl-CoA | - |
? | |
ATP + 3-methylthiopropionate + CoA | - |
Thermococcus kodakarensis | ADP + phosphate + 3-methylthiopropionyl-CoA | - |
? | |
ATP + acetate + CoA | - |
Thermococcus kodakarensis | ADP + phosphate + acetyl-CoA | - |
? | |
ATP + isobutyrate + CoA | - |
Thermococcus kodakarensis | ADP + phosphate + isobutyryl-CoA | - |
? | |
ATP + isovalerate + CoA | - |
Thermococcus kodakarensis | ADP + phosphate + isovaleryl-CoA | - |
? | |
ATP + phenylacetate + CoA | - |
Thermococcus kodakarensis | ADP + phosphate + phenylacetyl-CoA | - |
? | |
ATP + thioglycolate + CoA | - |
Thermococcus kodakarensis | ADP + phosphate + thioglycolyl-CoA | - |
? | |
additional information | almost no activity with formate, 5-aminovalerate, 4-guanidinobutyrate, 2-(imidazol-4-yl)acetate, malonate, and succinate | Thermococcus kodakarensis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACS III | isoform | Thermococcus kodakarensis |
ADP-forming acyl coenzyme A synthetase | - |
Thermococcus kodakarensis |
ADP-forming acyl-CoA synthetase | - |
Thermococcus kodakarensis |
TK0944/TK0943 protein | - |
Thermococcus kodakarensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26.1 | - |
thioglycolate | at pH 6.5 and 55°C | Thermococcus kodakarensis | |
40.6 | - |
phenylacetate | at pH 6.5 and 55°C | Thermococcus kodakarensis | |
49.3 | - |
acetate | at pH 6.5 and 55°C | Thermococcus kodakarensis | |
50.9 | - |
Isobutyrate | at pH 6.5 and 55°C | Thermococcus kodakarensis | |
53.1 | - |
CoA | at pH 6.5 and 55°C | Thermococcus kodakarensis | |
53.7 | - |
ATP | at pH 6.5 and 55°C | Thermococcus kodakarensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermococcus kodakarensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32.8 | - |
acetate | at pH 6.5 and 55°C | Thermococcus kodakarensis | |
81 | - |
thioglycolate | at pH 6.5 and 55°C | Thermococcus kodakarensis | |
123 | - |
phenylacetate | at pH 6.5 and 55°C | Thermococcus kodakarensis | |
385 | - |
Isobutyrate | at pH 6.5 and 55°C | Thermococcus kodakarensis | |
965 | - |
ATP | at pH 6.5 and 55°C | Thermococcus kodakarensis | |
9490 | - |
CoA | at pH 6.5 and 55°C | Thermococcus kodakarensis |