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Biochemical and kinetic characterization of the recombinant ADP-forming acetyl coenzyme A synthetase from the amitochondriate protozoan Entamoeba histolytica

Jones, C.P.; Ingram-Smith, C.; Eukaryot. Cell 13, 1530-1537 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli Rosetta2(DE3)pLysS cells
Entamoeba histolytica
Inhibitors
Inhibitors
Commentary
Organism
Structure
ATP
inhibits the acetate-forming direction of the reaction
Entamoeba histolytica
Ca2+
about 3% residual activity at 20 mM
Entamoeba histolytica
Cu2+
about 15% residual activity at 20 mM
Entamoeba histolytica
D-glucose 6-phosphate
-
Entamoeba histolytica
diphosphate
inhibits the acetate-forming direction of the reaction (64% residual activity at 10 mM)
Entamoeba histolytica
glyoxylate
32% residual activity at 10 mM
Entamoeba histolytica
NAD+
47% residual activity at 10 mM
Entamoeba histolytica
Ni2+
about 3% residual activity at 20 mM
Entamoeba histolytica
Zn2+
about 5% residual activity at 20 mM
Entamoeba histolytica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.032
-
propionyl-CoA
at pH 7.3 and 37°C
Entamoeba histolytica
0.04
-
acetyl-CoA
at pH 7.3 and 37°C
Entamoeba histolytica
0.2
-
CoA
with acetate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
0.71
-
ADP
with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
1.5
-
phosphate
with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
1.6
-
ADP
with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
1.6
-
CoA
with propionate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
1.8
-
phosphate
with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
1.9
-
GDP
with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
7.2
-
ATP
with propionate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
10
-
GTP
with acetate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
12
-
ATP
with acetate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
14
-
acetate
at pH 7.3 and 37°C
Entamoeba histolytica
29
-
propionate
at pH 7.3 and 37°C
Entamoeba histolytica
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
90% activity in the presence of 20 mM
Entamoeba histolytica
Mg2+
100% activity in the presence of 20 mM
Entamoeba histolytica
Mn2+
100% activity in the presence of 20 mM
Entamoeba histolytica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
150000
-
gel filtration
Entamoeba histolytica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ADP + phosphate + acetyl-CoA
Entamoeba histolytica
the specific activity in the acetate-forming direction is approximately half that observed in the acetyl-CoA-forming direction
ATP + acetate + CoA
-
-
r
ATP + acetate + CoA
Entamoeba histolytica
preferred direction of reaction
ADP + phosphate + acetyl-CoA
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Entamoeba histolytica
C4LUV9
-
-
Purification (Commentary)
Commentary
Organism
HisTrap HP nickel affinity column chromatography, and Superdex 200 gel filtration
Entamoeba histolytica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + phosphate + acetyl-CoA
100% activity
744820
Entamoeba histolytica
ATP + acetate + CoA
-
-
-
r
ADP + phosphate + acetyl-CoA
the specific activity in the acetate-forming direction is approximately half that observed in the acetyl-CoA-forming direction
744820
Entamoeba histolytica
ATP + acetate + CoA
-
-
-
r
ADP + phosphate + isobutyryl-CoA
less than 1% activity compared to acetyl-CoA
744820
Entamoeba histolytica
ATP + isobutyrate + CoA
-
-
-
r
ADP + phosphate + isovaleryl-CoA
less than 1% activity compared to acetyl-CoA
744820
Entamoeba histolytica
ATP + isovalerate + CoA
-
-
-
r
ADP + phosphate + propionyl-CoA
16% activity compared to acetyl-CoA
744820
Entamoeba histolytica
ATP + propionate + CoA
-
-
-
r
ATP + acetate + CoA
100% activity
744820
Entamoeba histolytica
ADP + phosphate + acetyl-CoA
-
-
-
r
ATP + acetate + CoA
preferred direction of reaction
744820
Entamoeba histolytica
ADP + phosphate + acetyl-CoA
-
-
-
r
ATP + butyrate + CoA
5.6% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + butyryl-CoA
-
-
-
r
ATP + heptanoate + CoA
less than 4% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + heptanoyl-CoA
-
-
-
r
ATP + hexanoate + CoA
less than 4% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + hexanoyl-CoA
-
-
-
r
ATP + isobutyrate + CoA
less than 4% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + isobutyryl-CoA
-
-
-
r
ATP + isovalerate + CoA
less than 4% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + isovaleryl-CoA
-
-
-
r
ATP + octanoate + CoA
less than 4% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + octanoyl-CoA
-
-
-
r
ATP + phenylacetate + CoA
less than 4% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + phenylacetyl-CoA
-
-
-
r
ATP + propionate + CoA
72% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + propionyl-CoA
-
-
-
r
ATP + succinate + CoA
less than 4% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + succinyl-CoA
-
-
-
r
ATP + valerate + CoA
less than 4% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + valeryl-CoA
-
-
-
r
CDP + phosphate + acetyl-CoA
7% activity compared to ADP
744820
Entamoeba histolytica
CTP + acetate + CoA
-
-
-
r
CTP + acetate + CoA
4.1% activity compared to ATP
744820
Entamoeba histolytica
CDP + phosphate + acetyl-CoA
-
-
-
r
GDP + phosphate + acetyl-CoA
106% activity compared to ADP
744820
Entamoeba histolytica
GTP + acetate + CoA
-
-
-
r
GTP + acetate + CoA
57% activity compared to ATP
744820
Entamoeba histolytica
GDP + phosphate + acetyl-CoA
-
-
-
r
IDP + phosphate + acetyl-CoA
28% activity compared to ADP
744820
Entamoeba histolytica
ITP + acetate + CoA
-
-
-
r
ITP + acetate + CoA
15% activity compared to ATP
744820
Entamoeba histolytica
IDP + phosphate + acetyl-CoA
-
-
-
r
additional information
no activity with diphosphate, AMP, TDP, TTP, butyryl-CoA, succinyl-CoA, or phenylacetyl-CoA
744820
Entamoeba histolytica
?
-
-
-
-
UDP + phosphate + acetyl-CoA
2.8% activity compared to ADP
744820
Entamoeba histolytica
UTP + acetate + CoA
-
-
-
r
UTP + acetate + CoA
1.6% activity compared to ATP
744820
Entamoeba histolytica
UDP + phosphate + acetyl-CoA
-
-
-
r
Subunits
Subunits
Commentary
Organism
homodimer
2 * 78999, calculated from amino acid sequence
Entamoeba histolytica
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Entamoeba histolytica
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
37
-
the enzyme exhibits 70% activity at 37°C
Entamoeba histolytica
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
18
-
phosphate
with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
21
-
propionyl-CoA
at pH 7.3 and 37°C
Entamoeba histolytica
24
-
ADP
with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
110
-
acetyl-CoA
at pH 7.3 and 37°C
Entamoeba histolytica
120
-
phosphate
with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
140
-
ADP
with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
170
-
GDP
with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
180
-
GTP
with acetate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
190
-
propionate
at pH 7.3 and 37°C
Entamoeba histolytica
220
-
CoA
with acetate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
240
-
acetate
at pH 7.3 and 37°C
Entamoeba histolytica
240
-
ATP
with propionate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
260
-
CoA
with propionate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
320
-
ATP
with acetate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
Cofactor
Cofactor
Commentary
Organism
Structure
ADP
-
Entamoeba histolytica
ATP
-
Entamoeba histolytica
GDP
-
Entamoeba histolytica
GTP
-
Entamoeba histolytica
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.81
-
at pH 7.3 and 37°C
Entamoeba histolytica
ATP
0.83
-
at pH 7.3 and 37°C
Entamoeba histolytica
diphosphate
10
-
at pH 7.3 and 37°C
Entamoeba histolytica
D-glucose 6-phosphate
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli Rosetta2(DE3)pLysS cells
Entamoeba histolytica
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ADP
-
Entamoeba histolytica
ATP
-
Entamoeba histolytica
GDP
-
Entamoeba histolytica
GTP
-
Entamoeba histolytica
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.81
-
at pH 7.3 and 37°C
Entamoeba histolytica
ATP
0.83
-
at pH 7.3 and 37°C
Entamoeba histolytica
diphosphate
10
-
at pH 7.3 and 37°C
Entamoeba histolytica
D-glucose 6-phosphate
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ATP
inhibits the acetate-forming direction of the reaction
Entamoeba histolytica
Ca2+
about 3% residual activity at 20 mM
Entamoeba histolytica
Cu2+
about 15% residual activity at 20 mM
Entamoeba histolytica
D-glucose 6-phosphate
-
Entamoeba histolytica
diphosphate
inhibits the acetate-forming direction of the reaction (64% residual activity at 10 mM)
Entamoeba histolytica
glyoxylate
32% residual activity at 10 mM
Entamoeba histolytica
NAD+
47% residual activity at 10 mM
Entamoeba histolytica
Ni2+
about 3% residual activity at 20 mM
Entamoeba histolytica
Zn2+
about 5% residual activity at 20 mM
Entamoeba histolytica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.032
-
propionyl-CoA
at pH 7.3 and 37°C
Entamoeba histolytica
0.04
-
acetyl-CoA
at pH 7.3 and 37°C
Entamoeba histolytica
0.2
-
CoA
with acetate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
0.71
-
ADP
with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
1.5
-
phosphate
with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
1.6
-
ADP
with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
1.6
-
CoA
with propionate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
1.8
-
phosphate
with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
1.9
-
GDP
with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
7.2
-
ATP
with propionate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
10
-
GTP
with acetate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
12
-
ATP
with acetate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
14
-
acetate
at pH 7.3 and 37°C
Entamoeba histolytica
29
-
propionate
at pH 7.3 and 37°C
Entamoeba histolytica
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
90% activity in the presence of 20 mM
Entamoeba histolytica
Mg2+
100% activity in the presence of 20 mM
Entamoeba histolytica
Mn2+
100% activity in the presence of 20 mM
Entamoeba histolytica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
150000
-
gel filtration
Entamoeba histolytica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ADP + phosphate + acetyl-CoA
Entamoeba histolytica
the specific activity in the acetate-forming direction is approximately half that observed in the acetyl-CoA-forming direction
ATP + acetate + CoA
-
-
r
ATP + acetate + CoA
Entamoeba histolytica
preferred direction of reaction
ADP + phosphate + acetyl-CoA
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
HisTrap HP nickel affinity column chromatography, and Superdex 200 gel filtration
Entamoeba histolytica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + phosphate + acetyl-CoA
100% activity
744820
Entamoeba histolytica
ATP + acetate + CoA
-
-
-
r
ADP + phosphate + acetyl-CoA
the specific activity in the acetate-forming direction is approximately half that observed in the acetyl-CoA-forming direction
744820
Entamoeba histolytica
ATP + acetate + CoA
-
-
-
r
ADP + phosphate + isobutyryl-CoA
less than 1% activity compared to acetyl-CoA
744820
Entamoeba histolytica
ATP + isobutyrate + CoA
-
-
-
r
ADP + phosphate + isovaleryl-CoA
less than 1% activity compared to acetyl-CoA
744820
Entamoeba histolytica
ATP + isovalerate + CoA
-
-
-
r
ADP + phosphate + propionyl-CoA
16% activity compared to acetyl-CoA
744820
Entamoeba histolytica
ATP + propionate + CoA
-
-
-
r
ATP + acetate + CoA
100% activity
744820
Entamoeba histolytica
ADP + phosphate + acetyl-CoA
-
-
-
r
ATP + acetate + CoA
preferred direction of reaction
744820
Entamoeba histolytica
ADP + phosphate + acetyl-CoA
-
-
-
r
ATP + butyrate + CoA
5.6% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + butyryl-CoA
-
-
-
r
ATP + heptanoate + CoA
less than 4% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + heptanoyl-CoA
-
-
-
r
ATP + hexanoate + CoA
less than 4% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + hexanoyl-CoA
-
-
-
r
ATP + isobutyrate + CoA
less than 4% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + isobutyryl-CoA
-
-
-
r
ATP + isovalerate + CoA
less than 4% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + isovaleryl-CoA
-
-
-
r
ATP + octanoate + CoA
less than 4% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + octanoyl-CoA
-
-
-
r
ATP + phenylacetate + CoA
less than 4% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + phenylacetyl-CoA
-
-
-
r
ATP + propionate + CoA
72% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + propionyl-CoA
-
-
-
r
ATP + succinate + CoA
less than 4% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + succinyl-CoA
-
-
-
r
ATP + valerate + CoA
less than 4% activity compared to acetate
744820
Entamoeba histolytica
ADP + phosphate + valeryl-CoA
-
-
-
r
CDP + phosphate + acetyl-CoA
7% activity compared to ADP
744820
Entamoeba histolytica
CTP + acetate + CoA
-
-
-
r
CTP + acetate + CoA
4.1% activity compared to ATP
744820
Entamoeba histolytica
CDP + phosphate + acetyl-CoA
-
-
-
r
GDP + phosphate + acetyl-CoA
106% activity compared to ADP
744820
Entamoeba histolytica
GTP + acetate + CoA
-
-
-
r
GTP + acetate + CoA
57% activity compared to ATP
744820
Entamoeba histolytica
GDP + phosphate + acetyl-CoA
-
-
-
r
IDP + phosphate + acetyl-CoA
28% activity compared to ADP
744820
Entamoeba histolytica
ITP + acetate + CoA
-
-
-
r
ITP + acetate + CoA
15% activity compared to ATP
744820
Entamoeba histolytica
IDP + phosphate + acetyl-CoA
-
-
-
r
additional information
no activity with diphosphate, AMP, TDP, TTP, butyryl-CoA, succinyl-CoA, or phenylacetyl-CoA
744820
Entamoeba histolytica
?
-
-
-
-
UDP + phosphate + acetyl-CoA
2.8% activity compared to ADP
744820
Entamoeba histolytica
UTP + acetate + CoA
-
-
-
r
UTP + acetate + CoA
1.6% activity compared to ATP
744820
Entamoeba histolytica
UDP + phosphate + acetyl-CoA
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 78999, calculated from amino acid sequence
Entamoeba histolytica
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Entamoeba histolytica
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
37
-
the enzyme exhibits 70% activity at 37°C
Entamoeba histolytica
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
18
-
phosphate
with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
21
-
propionyl-CoA
at pH 7.3 and 37°C
Entamoeba histolytica
24
-
ADP
with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
110
-
acetyl-CoA
at pH 7.3 and 37°C
Entamoeba histolytica
120
-
phosphate
with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
140
-
ADP
with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
170
-
GDP
with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
180
-
GTP
with acetate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
190
-
propionate
at pH 7.3 and 37°C
Entamoeba histolytica
220
-
CoA
with acetate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
240
-
acetate
at pH 7.3 and 37°C
Entamoeba histolytica
240
-
ATP
with propionate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
260
-
CoA
with propionate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
320
-
ATP
with acetate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.3
-
propionate
at pH 7.3 and 37°C
Entamoeba histolytica
12
-
phosphate
with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
16
-
acetate
at pH 7.3 and 37°C
Entamoeba histolytica
18
-
GTP
with acetate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
27
-
ATP
with acetate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
33
-
ATP
with propionate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
34
-
ADP
with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
65
-
phosphate
with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
89
-
ADP
with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
90
-
GDP
with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
160
-
CoA
with propionate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
650
-
propionyl-CoA
at pH 7.3 and 37°C
Entamoeba histolytica
1100
-
CoA
with acetate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
2600
-
acetyl-CoA
at pH 7.3 and 37°C
Entamoeba histolytica
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.3
-
propionate
at pH 7.3 and 37°C
Entamoeba histolytica
12
-
phosphate
with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
16
-
acetate
at pH 7.3 and 37°C
Entamoeba histolytica
18
-
GTP
with acetate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
27
-
ATP
with acetate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
33
-
ATP
with propionate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
34
-
ADP
with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
65
-
phosphate
with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
89
-
ADP
with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
90
-
GDP
with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
160
-
CoA
with propionate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
650
-
propionyl-CoA
at pH 7.3 and 37°C
Entamoeba histolytica
1100
-
CoA
with acetate as cosubstrate, at pH 7.3 and 37°C
Entamoeba histolytica
2600
-
acetyl-CoA
at pH 7.3 and 37°C
Entamoeba histolytica
Other publictions for EC 6.2.1.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744911
Jones
Investigating the mechanism o ...
Entamoeba histolytica
FEBS Lett.
591
603-612
2017
-
-
1
-
14
-
-
46
-
1
-
4
-
2
-
-
1
1
-
-
-
-
5
1
-
-
-
48
-
-
-
2
-
-
-
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-
1
3
-
17
-
-
-
-
46
-
1
-
4
-
-
-
1
-
-
-
-
5
1
-
-
-
48
-
-
-
-
-
2
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48
48
744414
Pineda
Roles of acetyl-CoA synthetas ...
Entamoeba histolytica
Biochim. Biophys. Acta
1860
1163-1172
2016
-
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1
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1
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-
13
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-
2
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-
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3
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1
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8
3
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-
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-
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2
-
-
-
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1
-
1
3
1
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-
-
8
3
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-
-
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2
2
-
12
12
746438
Barnhart
Potential role of acetyl-CoA ...
Methanosarcina acetivorans
Sci. Rep.
5
12498
2015
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744820
Jones
Biochemical and kinetic chara ...
Entamoeba histolytica
Eukaryot. Cell
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1530-1537
2014
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14
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26
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Characterization of two membe ...
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140-147
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Acetate formation in the photo ...
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Chan
In Salmonella enterica, the si ...
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Reaction mechanism and structu ...
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Citric acid cycle in the hyper ...
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4350-4355
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Lehtioe
Unusual twinning in an acetyl ...
Pyrococcus furiosus
Acta Crystallogr. Sect. D
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350-354
2005
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An acetyl-CoA synthetase not e ...
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663-669
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4
2
2
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1
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4
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2
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1
-
-
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Unusual ADP-forming acetyl-coe ...
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10
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1
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2
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Bräsen
Regulation of acetate and acet ...
Haloarcula marismortui
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Mechanisms of acetate formatio ...
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1
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1
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1
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1
-
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1
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-
-
3
3
1
3
3
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-
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3
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2
5
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5
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1
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Sugar utilization in the hyper ...
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Cloning and sequencing of an a ...
Giardia intestinalis, Giardia intestinalis WB / CC 30957
Gene
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Acetyl coenzyme A synthetase ( ...
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Purification and properties of ...
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Purification and characterizat ...
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Purification and characterizat ...
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Maltose fermentation to aceta ...
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An energy-conserving pyruvate- ...
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