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Literature summary for 6.2.1.13 extracted from

  • De Cima, S.; Rua, J.; Perdiguero, E.; del Valle, P.; Busto, F.; Baroja-Mazo, A.; de Arriaga, D.
    An acetyl-CoA synthetase not encoded by the facA gene is expressed under carbon starvation in Phycomyces blakesleeanus (2005), Res. Microbiol., 156, 663-669.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
acetate 30°C, isoenzyme ACS1 Phycomyces blakesleeanus
1.02
-
acetate pH 8.0, 37°C, isoenzyme ACS2 Phycomyces blakesleeanus
5.2
-
propionate pH 8.0, 37°C, isoenzyme ACS2 Phycomyces blakesleeanus
8.55
-
propionate 30°C, isoenzyme ACS1 Phycomyces blakesleeanus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates, 45% of the efficiency of Mg2+ and Mn2+ Phycomyces blakesleeanus
Co2+ activates, 90% of the efficiency of Mg2+ and Mn2+ Phycomyces blakesleeanus
Mg2+ activates Phycomyces blakesleeanus
Mn2+ activates Phycomyces blakesleeanus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, isoenzyme ACS1, SDS-PAGE Phycomyces blakesleeanus
77000
-
x * 77000, isoenzyme ACS2, SDS-PAGE Phycomyces blakesleeanus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Phycomyces blakesleeanus ACS1, encoded by the gene facA, is induced by acetate and repressed by glucose at the transcriptional level ?
-
?
additional information Phycomyces blakesleeanus ACS2 may be a stress protein, expressed under carbon source starvation ?
-
?

Organism

Organism UniProt Comment Textmining
Phycomyces blakesleeanus
-
enzyme form ACS1
-
Phycomyces blakesleeanus
-
enzyme form ACS2
-

Purification (Commentary)

Purification (Comment) Organism
partial, isoenzyme ACS1 Phycomyces blakesleeanus
partial, isoenzyme ACS2 Phycomyces blakesleeanus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Phycomyces blakesleeanus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate + CoA
-
Phycomyces blakesleeanus ADP + phosphate + acetyl-CoA
-
?
ATP + propionate + CoA
-
Phycomyces blakesleeanus ADP + phosphate + propionyl-CoA
-
?
additional information ACS1, encoded by the gene facA, is induced by acetate and repressed by glucose at the transcriptional level Phycomyces blakesleeanus ?
-
?
additional information ACS2 may be a stress protein, expressed under carbon source starvation Phycomyces blakesleeanus ?
-
?

Subunits

Subunits Comment Organism
? x * 70000, isoenzyme ACS1, SDS-PAGE Phycomyces blakesleeanus
? x * 77000, isoenzyme ACS2, SDS-PAGE Phycomyces blakesleeanus

Synonyms

Synonyms Comment Organism
ACS1
-
Phycomyces blakesleeanus
ACS2
-
Phycomyces blakesleeanus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30 isoenzyme ACS1 Phycomyces blakesleeanus
50
-
isoenzyme ACS2 Phycomyces blakesleeanus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 20°C: about 75% of maximal activity, 40°C: about 45% of maximal activity, isoenzyme ACS1 Phycomyces blakesleeanus
25 55 25°C: about 45% of maximal activity, 55°C: about 40% of maximal activity, isoenzyme ACS2 Phycomyces blakesleeanus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
Phycomyces blakesleeanus