KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
acetate | 30°C, isoenzyme ACS1 | Phycomyces blakesleeanus | |
1.02 | - |
acetate | pH 8.0, 37°C, isoenzyme ACS2 | Phycomyces blakesleeanus | |
5.2 | - |
propionate | pH 8.0, 37°C, isoenzyme ACS2 | Phycomyces blakesleeanus | |
8.55 | - |
propionate | 30°C, isoenzyme ACS1 | Phycomyces blakesleeanus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates, 45% of the efficiency of Mg2+ and Mn2+ | Phycomyces blakesleeanus | |
Co2+ | activates, 90% of the efficiency of Mg2+ and Mn2+ | Phycomyces blakesleeanus | |
Mg2+ | activates | Phycomyces blakesleeanus | |
Mn2+ | activates | Phycomyces blakesleeanus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, isoenzyme ACS1, SDS-PAGE | Phycomyces blakesleeanus |
77000 | - |
x * 77000, isoenzyme ACS2, SDS-PAGE | Phycomyces blakesleeanus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Phycomyces blakesleeanus | ACS1, encoded by the gene facA, is induced by acetate and repressed by glucose at the transcriptional level | ? | - |
? | |
additional information | Phycomyces blakesleeanus | ACS2 may be a stress protein, expressed under carbon source starvation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phycomyces blakesleeanus | - |
enzyme form ACS1 | - |
Phycomyces blakesleeanus | - |
enzyme form ACS2 | - |
Purification (Comment) | Organism |
---|---|
partial, isoenzyme ACS1 | Phycomyces blakesleeanus |
partial, isoenzyme ACS2 | Phycomyces blakesleeanus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Phycomyces blakesleeanus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | - |
Phycomyces blakesleeanus | ADP + phosphate + acetyl-CoA | - |
? | |
ATP + propionate + CoA | - |
Phycomyces blakesleeanus | ADP + phosphate + propionyl-CoA | - |
? | |
additional information | ACS1, encoded by the gene facA, is induced by acetate and repressed by glucose at the transcriptional level | Phycomyces blakesleeanus | ? | - |
? | |
additional information | ACS2 may be a stress protein, expressed under carbon source starvation | Phycomyces blakesleeanus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, isoenzyme ACS1, SDS-PAGE | Phycomyces blakesleeanus |
? | x * 77000, isoenzyme ACS2, SDS-PAGE | Phycomyces blakesleeanus |
Synonyms | Comment | Organism |
---|---|---|
ACS1 | - |
Phycomyces blakesleeanus |
ACS2 | - |
Phycomyces blakesleeanus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | isoenzyme ACS1 | Phycomyces blakesleeanus |
50 | - |
isoenzyme ACS2 | Phycomyces blakesleeanus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | 20°C: about 75% of maximal activity, 40°C: about 45% of maximal activity, isoenzyme ACS1 | Phycomyces blakesleeanus |
25 | 55 | 25°C: about 45% of maximal activity, 55°C: about 40% of maximal activity, isoenzyme ACS2 | Phycomyces blakesleeanus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Phycomyces blakesleeanus |