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Literature summary for 6.2.1.13 extracted from
- Mechanisms of acetate formation and acetate activation in halophilic archaea (2001), Arch. Microbiol., 175, 360-368.
General Stability
| General Stability | Organism |
|---|---|
| stability for more than 48 requires high salt concentrations, i.e., 1-2 M of KCl or NaCl | Halococcus saccharolyticus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.41 | - |
CoA | pH 9.0, at 37ºC, oxic conditions | Halococcus saccharolyticus |  |
| 1 | - |
ATP | pH 9.0, at 37ºC, oxic conditions | Halococcus saccharolyticus | |
| 5.3 | - |
acetate | pH 9.0, at 37ºC, oxic conditions | Halococcus saccharolyticus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Haloferax volcanii | - |
- |
| soluble | - |
Halococcus saccharolyticus | - |
- |
| soluble | - |
Halorubrum saccharovorum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, 30 mM for maximal catalytic activity | Halococcus saccharolyticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 140000 | 150000 | gel filtration | Haloferax volcanii |
| 140000 | 150000 | gel filtration | Halococcus saccharolyticus |
| 140000 | 150000 | gel filtration | Halorubrum saccharovorum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphate + acetyl-CoA | Haloferax volcanii | - |
ATP + acetate + CoA | - |
ir | |
| ADP + phosphate + acetyl-CoA | Halococcus saccharolyticus | - |
ATP + acetate + CoA | - |
r | |
| ADP + phosphate + acetyl-CoA | Halorubrum saccharovorum | - |
ATP + acetate + CoA | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halococcus saccharolyticus | - |
strain DSM 5350 | - |
| Haloferax volcanii | - |
strain DSM 3757 | - |
| Halorubrum saccharovorum | - |
strain DSM 1137 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration on a Superdex TM200 column | Haloferax volcanii |
| gel filtration on a Superdex TM200 column | Halococcus saccharolyticus |
| gel filtration on a Superdex TM200 column | Halorubrum saccharovorum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Haloferax volcanii | - |
| cell culture | - |
Halorubrum saccharovorum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.03 | - |
pH 9.0, at 37ºC, oxic conditions, during stationary phase on glucose | Halococcus saccharolyticus |
| 0.04 | - |
pH 9.0, at 37ºC, oxic conditions, during exponential growth phase on glucose | Halococcus saccharolyticus |
| 0.1 | - |
pH 9.0, at 37ºC, oxic conditions, during exponential growth phase on glucose | Haloferax volcanii |
| 0.1 | - |
pH 9.0, at 37ºC, oxic conditions, during exponential growth phase on glucose | Halorubrum saccharovorum |
| 0.45 | - |
pH 9.0, at 37ºC, oxic conditions, purified enzyme | Halococcus saccharolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphate + acetyl-CoA | - |
Haloferax volcanii | ATP + acetate + CoA | - |
ir | |
| ADP + phosphate + acetyl-CoA | - |
Haloferax volcanii | ATP + acetate + CoA | - |
r | |
| ADP + phosphate + acetyl-CoA | - |
Halococcus saccharolyticus | ATP + acetate + CoA | - |
r | |
| ADP + phosphate + acetyl-CoA | - |
Halorubrum saccharovorum | ATP + acetate + CoA | - |
ir | |
| ADP + phosphate + acetyl-CoA | - |
Halorubrum saccharovorum | ATP + acetate + CoA | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADP-ACS | - |
Haloferax volcanii |
| ADP-ACS | - |
Halococcus saccharolyticus |
| ADP-ACS | - |
Halorubrum saccharovorum |
| ADP-forming acetyl-CoA synthetase | - |
Haloferax volcanii |
| ADP-forming acetyl-CoA synthetase | - |
Halococcus saccharolyticus |
| ADP-forming acetyl-CoA synthetase | - |
Halorubrum saccharovorum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 43 | - |
- |
Halococcus saccharolyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Halococcus saccharolyticus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | oxidation to NAD+ by assay system lactate dehydrogenase and pyruvate kinase | Halococcus saccharolyticus | |
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