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Literature summary for 6.2.1.13 extracted from
- Purification and characterization of two reversible and ADP-dependent acetyl coenzyme A synthetases from the hyperthermophilic archaeon Pyrococcus furiosus (1996), J. Bacteriol., 178, 5897-5903.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
phenylacetyl-CoA | isoenzyme ACS II | Pyrococcus furiosus |  |
| 0.012 | - |
isobutyryl-CoA | isoenzyme ACS II | Pyrococcus furiosus | |
| 0.018 | - |
CoA | isoenzyme ACS I | Pyrococcus furiosus | |
| 0.025 | - |
acetyl-CoA | isoenzyme ACS I | Pyrococcus furiosus | |
| 0.026 | - |
acetyl-CoA | isoenzyme ACS II | Pyrococcus furiosus | |
| 0.029 | - |
isobutyryl-CoA | isoenzyme ACS I | Pyrococcus furiosus | |
| 0.061 | - |
ADP | isoenzyme ACS II | Pyrococcus furiosus | |
| 0.132 | - |
GDP | isoenzyme ACS I | Pyrococcus furiosus | |
| 0.15 | - |
ADP | isoenzyme ACS I | Pyrococcus furiosus | |
| 0.236 | - |
GDP | isoenzyme ACS II | Pyrococcus furiosus | |
| 0.396 | - |
phosphate | isoenzyme ACS I | Pyrococcus furiosus | |
| 0.43 | - |
GTP | isoenzyme ACS I | Pyrococcus furiosus | |
| 0.457 | - |
Isobutyrate | isoenzyme ACS I | Pyrococcus furiosus | |
| 0.477 | - |
ATP | isoenzyme ACS I | Pyrococcus furiosus | |
| 0.58 | - |
phosphate | isoenzyme ACS II | Pyrococcus furiosus | |
| 1.1 | - |
acetate | isoenzyme ACS I | Pyrococcus furiosus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | contains neither iron nor other metals, such as copper, zinc, or magnesium | Pyrococcus furiosus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
2 * 45000 (alpha) + 2 * 23000 (beta), SDS-PAGE | Pyrococcus furiosus |
| 45000 | - |
2 * 45000 (alpha) + 2 * 23000 (beta), SDS-PAGE | Pyrococcus furiosus |
| 140000 | - |
gel filtration | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
2 isoenzymes: ACS I and ACS II | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| stable to oxygen for 24 h | Pyrococcus furiosus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoenzymes ACS I and ACS II | Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30 | - |
isoenzyme ACS II | Pyrococcus furiosus |
| 64.6 | - |
isoenzyme ACS I | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphate + acetyl-CoA | r | Pyrococcus furiosus | ATP + acetate + CoA | - |
? | |
| ADP + phosphate + acetyl-CoA | isoenzymes ACS I and ACS II | Pyrococcus furiosus | ATP + acetate + CoA | - |
? | |
| ADP + phosphate + indoleacetyl-CoA | r, isoenzyme ACS II is active, ACS I not | Pyrococcus furiosus | ATP + indoleacetate + CoA | - |
? | |
| ADP + phosphate + phenylacetyl-CoA | r, isoenzyme ACS II is active, ACS I not | Pyrococcus furiosus | ATP + phenylacetate + CoA | - |
? | |
| ADP + phosphate + succinyl-CoA | no activity with isoenzymes ACS I and ACS II | Pyrococcus furiosus | ATP + succinate + CoA | - |
? | |
| ATP + isobutyrate + CoA | r | Pyrococcus furiosus | ADP + phosphate + isobutyryl-CoA | - |
? | |
| ATP + isobutyrate + CoA | isoenzymes ACS I and ACS II | Pyrococcus furiosus | ADP + phosphate + isobutyryl-CoA | - |
? | |
| GDP + phosphate + acetyl-CoA | - |
Pyrococcus furiosus | GTP + acetate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 45000 (alpha) + 2 * 23000 (beta), SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
above, at pH 8.0 | Pyrococcus furiosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
isoenzymes ACS I and ACS II are inactive at | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
t1/2: 18 h for isoenzyme ACS I, 8 h for isoenzyme ACS II | Pyrococcus furiosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8 | - |
isobutyryl-CoA | isoenzyme ACS II | Pyrococcus furiosus | |
| 21 | - |
GDP | isoenzyme ACS II | Pyrococcus furiosus | |
| 22 | - |
Isobutyrate | isoenzyme ACS II | Pyrococcus furiosus | |
| 27 | - |
GTP | isoenzyme ACS II | Pyrococcus furiosus | |
| 42 | - |
acetyl-CoA | isoenzyme ACS II | Pyrococcus furiosus | |
| 55 | - |
Isobutyrate | isoenzyme ACS I | Pyrococcus furiosus | |
| 65 | - |
CoA | isoenzyme ACS I | Pyrococcus furiosus | |
| 66 | - |
indoleacetate | isoenzyme ACS II | Pyrococcus furiosus | |
| 67 | - |
acetate | isoenzyme ACS II | Pyrococcus furiosus | |
| 68 | - |
ATP | isoenzyme ACS II | Pyrococcus furiosus | |
| 70 | - |
CoA | isoenzyme ACS II | Pyrococcus furiosus | |
| 73 | - |
CoA | isoenzyme ACS I | Pyrococcus furiosus | |
| 82 | - |
ATP | isoenzyme ACS I | Pyrococcus furiosus | |
| 89 | - |
phenylacetate | isoenzyme ACS II | Pyrococcus furiosus | |
| 115 | - |
ADP | isoenzyme ACS II | Pyrococcus furiosus | |
| 117 | - |
phosphate | isoenzyme ACS II | Pyrococcus furiosus | |
| 121 | - |
isobutyryl-CoA | GTP, isoenzyme ACS I | Pyrococcus furiosus | |
| 138 | - |
phenylacetyl-CoA | isoenzyme ACS II | Pyrococcus furiosus | |
| 157 | - |
acetyl-CoA | isoenzyme ACS I | Pyrococcus furiosus | |
| 182 | - |
phosphate | isoenzyme ACS I | Pyrococcus furiosus | |
| 203 | - |
ADP | isoenzyme ACS I | Pyrococcus furiosus | |
| 411 | - |
GDP | isoenzyme ACS I | Pyrococcus furiosus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
at 80°C | Pyrococcus furiosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | 6: about 60% of maximal activity, 11: about 40% of maximal activity, isoenzyme ACS I | Pyrococcus furiosus |
| 7 | 11 | 7: about 60% of maximal activity, 11: about 65% of maximal activity, isoenzyme ACS II | Pyrococcus furiosus |
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