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Literature summary for 6.2.1.13 extracted from

  • Glasemacher, J.; Bock, A.K.; Schmid, R.; Schönheit, P.
    Purification and properties of acetyl-CoA synthetase (ADP-forming), an archaeal enzyme of acetate formation and ATP synthesis, from the hyperthermophile Pyrococcus furiosus (1997), Eur. J. Biochem., 244, 561-567.
    View publication on PubMed
Search for more literature for 6.2.1.13

General Stability

General Stability Organism
salts stabilize against heat inactivation. In presence of 1 M KCl the enzyme does not lose activity after 2 h incubation Pyrococcus furiosus
sensitivity towards heat inactivation is increased with storage at -20°C Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 acetyl-CoA
-
 Pyrococcus furiosus
0.03
-
 CoA
-
 Pyrococcus furiosus
0.06
-
 ADP
-
 Pyrococcus furiosus
0.08
-
 ADP
-
 Pyrococcus furiosus
0.2
-
 phosphate
-
 Pyrococcus furiosus
0.66
-
 acetate
-
 Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ no effect Pyrococcus furiosus
Co2+ can replace Mg2+ in activation Pyrococcus furiosus
Co2+ 44% of the activation relative to Mg2+ Pyrococcus furiosus
Mg2+ required Pyrococcus furiosus
Mg2+ Km: 0.2 mM Pyrococcus furiosus
Mg2+ an Mg2+/ATP ratio of up to 10 is required for optimal activity, suggesting that Mg2+ not only complexes ADP but has an additional stimulating effect on enzyme function Pyrococcus furiosus
Mn2+ can replace Mg2+ in activation Pyrococcus furiosus
Mn2+ 68% of the activation relative to Mg2+ Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 2 * 47000 (alpha) + 2 * 25000 (beta), SDS-PAGE Pyrococcus furiosus
47000
-
 2 * 47000 (alpha) + 2 * 25000 (beta), SDS-PAGE Pyrococcus furiosus
145000
-
 gel filtration Pyrococcus furiosus
150000 165000 native PAGE Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + phosphate + acetyl-CoA Pyrococcus furiosus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pyrococcus furiosus

Storage Stability

Storage Stability Organism
-20°C, 1-2 mg/ml enzyme, in 20 mM Tris/HCl, pH 8.0, 2 mM MgCl2, 150 mM NaCl, stable for several weeks Pyrococcus furiosus
on ice stable for 2 d Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphate + acetyl-CoA r Pyrococcus furiosus ATP + acetate + CoA
-
 ?
ADP + phosphate + acetyl-CoA
-
 Pyrococcus furiosus ?
-
 ?
ADP + phosphate + butyryl-CoA 92% of the activity relative to acetyl-CoA Pyrococcus furiosus ATP + butyrate + CoA
-
 ?
ADP + phosphate + propionyl-CoA r Pyrococcus furiosus ATP + propionate + CoA
-
 ?
ADP + phosphate + propionyl-CoA 110% of the activity relative to acetyl-CoA Pyrococcus furiosus ATP + propionate + CoA
-
 ?
ATP + isobutyrate + CoA 79% of the activity relative to acetate Pyrococcus furiosus ADP + phosphate + isobutyryl-CoA
-
 ?
ATP + isopentanioate + CoA 34% of the activity relative to acetate Pyrococcus furiosus ADP + phosphate + isovaleryl-CoA
-
 ?
ATP + pentanoate + CoA 36% of the activity relative to acetate Pyrococcus furiosus ADP + phosphate + valeryl-CoA
-
 ?
GDP + phosphate + acetyl-CoA r, 220% of the activity relative to ADP Pyrococcus furiosus GTP + acetate + CoA
-
 ?
IDP + phosphate + acetyl-CoA r, 250% of the activity relative to ADP Pyrococcus furiosus ITP + acetate + CoA
-
 ?

Subunits

Subunits Comment Organism
tetramer 2 * 47000 (alpha) + 2 * 25000 (beta), SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 half-life is 60 min, in presence of 1 M KCl no loss of activity after 2 h Pyrococcus furiosus
110
-
 half-life is 30 min Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
6 8 about 50% of maximal activity at pH 6 and 8 Pyrococcus furiosus