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Literature summary for 6.2.1.12 extracted from
- Divergent and overlapping function of five 4-coumarate/coenzyme A ligases from Populus tomentosa (2015), Plant Mol. Biol. Rep., 33, 841-854 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Nicotiana tabacum roots | Populus tomentosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| V388del | deletion of V338 residues in isoform 4CL1 results in activity towards sinapate | Populus tomentosa |
| Y236F | the mutant of isoform 4CL2 exhibits high substrate catalytic efficiency for 4-coumarate but very low substrate affinities and specificities for caffeate and ferulate | Populus tomentosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01271 | - |
4-coumarate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa |  |
| 0.02611 | - |
4-coumarate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.02631 | - |
caffeate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.02643 | - |
caffeate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.02699 | - |
ferulate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.0272 | - |
4-coumarate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.03516 | - |
ferulate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.04217 | - |
ferulate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.04414 | - |
caffeate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.05399 | - |
4-coumarate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.05679 | - |
4-coumarate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.06132 | - |
caffeate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.06868 | - |
ferulate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.07366 | - |
cinnamate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.08261 | - |
ferulate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.08417 | - |
caffeate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.13571 | - |
cinnamate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.14266 | - |
cinnamate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.19649 | - |
cinnamate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.20002 | - |
cinnamate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 4-coumarate + CoA | Populus tomentosa | - |
AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Populus tomentosa | H9AZ21 | - |
- |
| Populus tomentosa | H9AZ22 | - |
- |
| Populus tomentosa | H9AZ23 | - |
- |
| Populus tomentosa | H9AZ24 | - |
- |
| Populus tomentosa | Q941M3 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bud | - |
Populus tomentosa | - |
| leaf | - |
Populus tomentosa | - |
| leaf | highest expression of isoform 4CL2 | Populus tomentosa | - |
| leaf | highest expression of isoform 4CL4 | Populus tomentosa | - |
| additional information | isoform 4CL1 is not expressed in bud and phloem | Populus tomentosa | - |
| additional information | isoform 4CL2 is not expressed in xylem and root | Populus tomentosa | - |
| additional information | isoform 4CL4 is not expressed in phloem | Populus tomentosa | - |
| additional information | isoform 4CL5 is not expressed in phloem | Populus tomentosa | - |
| phloem | - |
Populus tomentosa | - |
| root | - |
Populus tomentosa | - |
| root | highest expression of isoform 4CL1 | Populus tomentosa | - |
| root | highest expression of isoform 4CL5 | Populus tomentosa | - |
| xylem | - |
Populus tomentosa | - |
| xylem | highest expression of isoform 4CL3 | Populus tomentosa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 4-coumarate + CoA | - |
Populus tomentosa | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
| ATP + caffeate + CoA | - |
Populus tomentosa | AMP + diphosphate + caffeoyl-CoA | - |
? | |
| ATP + cinnamate + CoA | - |
Populus tomentosa | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
| ATP + ferulate + CoA | - |
Populus tomentosa | AMP + diphosphate + feruloyl-CoA | - |
? | |
| ATP + ferulate + CoA | good substrate for isoform 4CL4 | Populus tomentosa | AMP + diphosphate + feruloyl-CoA | - |
? | |
| ATP + ferulate + CoA | good substrate for isoform 4CL5 | Populus tomentosa | AMP + diphosphate + feruloyl-CoA | - |
? | |
| additional information | no activity with sinapate | Populus tomentosa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4-coumarate/coenzyme A ligase | - |
Populus tomentosa |
| 4CL1 | isoform | Populus tomentosa |
| 4CL2 | isoform | Populus tomentosa |
| 4CL3 | isoform | Populus tomentosa |
| 4CL4 | isoform | Populus tomentosa |
| 4CL5 | isoform | Populus tomentosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
cinnamate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.61 | - |
cinnamate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
| 0.96 | - |
ferulate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
| 1.17 | - |
cinnamate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
| 1.43 | - |
caffeate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
| 1.51 | - |
cinnamate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
| 1.66 | - |
caffeate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
| 2.23 | - |
ferulate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
| 3.22 | - |
cinnamate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
| 3.71 | - |
4-coumarate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
| 4.19 | - |
ferulate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
| 4.24 | - |
4-coumarate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
| 4.55 | - |
4-coumarate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
| 5.81 | - |
caffeate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
| 5.84 | - |
caffeate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
| 6.07 | - |
ferulate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
| 6.84 | - |
4-coumarate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
| 31.4 | - |
caffeate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
| 88.68 | - |
4-coumarate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
| 126.47 | - |
ferulate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Populus tomentosa | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.45 | - |
cinnamate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
| 5.95 | - |
cinnamate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
| 8.28 | - |
cinnamate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
| 11.13 | - |
cinnamate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
| 13.98 | - |
ferulate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
| 16.1 | - |
cinnamate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
| 23.32 | - |
caffeate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
| 52.88 | - |
ferulate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
| 62.81 | - |
caffeate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
| 69.38 | - |
caffeate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
| 73.48 | - |
ferulate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
| 74.66 | - |
4-coumarate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
| 119.17 | - |
ferulate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
| 126.69 | - |
4-coumarate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
| 136.4 | - |
4-coumarate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
| 220.83 | - |
caffeate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
| 357.99 | - |
4-coumarate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
| 711.37 | - |
caffeate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
| 3396.4 | - |
4-coumarate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
| 4685.81 | - |
ferulate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
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