Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 (DE3) cells | Pinus taeda |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01097 | - |
4-coumarate | isoform 4CL3, pH and temperature not specified in the publication | Pinus taeda | |
0.0296 | - |
caffeate | isoform 4CL1, pH and temperature not specified in the publication | Pinus taeda | |
0.0302 | - |
4-coumarate | isoform 4CL1, pH and temperature not specified in the publication | Pinus taeda | |
0.0423 | - |
caffeate | isoform 4CL3, pH and temperature not specified in the publication | Pinus taeda | |
0.0522 | - |
ferulate | isoform 4CL1, pH and temperature not specified in the publication | Pinus taeda | |
0.0849 | - |
ferulate | isoform 4CL3, pH and temperature not specified in the publication | Pinus taeda | |
0.8875 | - |
cinnamate | isoform 4CL3, pH and temperature not specified in the publication | Pinus taeda | |
1.0803 | - |
cinnamate | isoform 4CL1, pH and temperature not specified in the publication | Pinus taeda |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | Pinus taeda | preferred substrate | AMP + diphosphate + 4-coumaroyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pinus taeda | P41636 | loblolly pine | - |
Pinus taeda | U5MY99 | loblolly pine | - |
Purification (Comment) | Organism |
---|---|
His-Bind purification kit | Pinus taeda |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Pinus taeda | - |
additional information | isoform 4CL3 is absent in xylem | Pinus taeda | - |
root | highest expression in root tip | Pinus taeda | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | - |
Pinus taeda | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | preferred substrate | Pinus taeda | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + caffeate + CoA | - |
Pinus taeda | AMP + diphosphate + caffeoyl-CoA | - |
? | |
ATP + cinnamate + CoA | - |
Pinus taeda | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
ATP + ferulate + CoA | - |
Pinus taeda | AMP + diphosphate + feruloyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-coumarate:CoA ligase | - |
Pinus taeda |
4CL1 | isoform | Pinus taeda |
4CL3 | isoform | Pinus taeda |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
caffeate | isoform 4CL3, pH and temperature not specified in the publication | Pinus taeda | |
0.15 | - |
ferulate | isoform 4CL3, pH and temperature not specified in the publication | Pinus taeda | |
0.19 | - |
4-coumarate | isoform 4CL3, pH and temperature not specified in the publication | Pinus taeda | |
0.27 | - |
cinnamate | isoform 4CL3, pH and temperature not specified in the publication | Pinus taeda | |
1.6 | - |
caffeate | isoform 4CL1, pH and temperature not specified in the publication | Pinus taeda | |
4.15 | - |
4-coumarate | isoform 4CL1, pH and temperature not specified in the publication | Pinus taeda | |
5.22 | - |
cinnamate | isoform 4CL1, pH and temperature not specified in the publication | Pinus taeda | |
14.99 | - |
ferulate | isoform 4CL1, pH and temperature not specified in the publication | Pinus taeda |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pinus taeda |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
cinnamate | isoform 4CL3, pH and temperature not specified in the publication | Pinus taeda | |
1.8 | - |
ferulate | isoform 4CL3, pH and temperature not specified in the publication | Pinus taeda | |
3.1 | - |
caffeate | isoform 4CL3, pH and temperature not specified in the publication | Pinus taeda | |
4.8 | - |
cinnamate | isoform 4CL1, pH and temperature not specified in the publication | Pinus taeda | |
17.3 | - |
4-coumarate | isoform 4CL3, pH and temperature not specified in the publication | Pinus taeda | |
54.1 | - |
caffeate | isoform 4CL1, pH and temperature not specified in the publication | Pinus taeda | |
137.3 | - |
4-coumarate | isoform 4CL1, pH and temperature not specified in the publication | Pinus taeda | |
287.4 | - |
ferulate | isoform 4CL1, pH and temperature not specified in the publication | Pinus taeda |