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Literature summary for 6.2.1.12 extracted from

  • Chen, H.Y.; Babst, B.A.; Nyamdari, B.; Hu, H.; Sykes, R.; Davis, M.F.; Harding, S.A.; Tsai, C.J.
    Ectopic expression of a loblolly pine class II 4-coumarate CoA ligase alters soluble phenylpropanoid metabolism but not lignin biosynthesis in Populus (2014), Plant Cell Physiol., 55, 1669-1678 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2 (DE3) cells Pinus taeda

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01097
-
4-coumarate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
0.0296
-
caffeate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda
0.0302
-
4-coumarate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda
0.0423
-
caffeate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
0.0522
-
ferulate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda
0.0849
-
ferulate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
0.8875
-
cinnamate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
1.0803
-
cinnamate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-coumarate + CoA Pinus taeda preferred substrate AMP + diphosphate + 4-coumaroyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Pinus taeda P41636 loblolly pine
-
Pinus taeda U5MY99 loblolly pine
-

Purification (Commentary)

Purification (Comment) Organism
His-Bind purification kit Pinus taeda

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Pinus taeda
-
additional information isoform 4CL3 is absent in xylem Pinus taeda
-
root highest expression in root tip Pinus taeda
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-coumarate + CoA
-
Pinus taeda AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + 4-coumarate + CoA preferred substrate Pinus taeda AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + caffeate + CoA
-
Pinus taeda AMP + diphosphate + caffeoyl-CoA
-
?
ATP + cinnamate + CoA
-
Pinus taeda AMP + diphosphate + cinnamoyl-CoA
-
?
ATP + ferulate + CoA
-
Pinus taeda AMP + diphosphate + feruloyl-CoA
-
?

Synonyms

Synonyms Comment Organism
4-coumarate:CoA ligase
-
Pinus taeda
4CL1 isoform Pinus taeda
4CL3 isoform Pinus taeda

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
caffeate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
0.15
-
ferulate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
0.19
-
4-coumarate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
0.27
-
cinnamate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
1.6
-
caffeate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda
4.15
-
4-coumarate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda
5.22
-
cinnamate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda
14.99
-
ferulate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda

Cofactor

Cofactor Comment Organism Structure
ATP
-
Pinus taeda

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.3
-
cinnamate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
1.8
-
ferulate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
3.1
-
caffeate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
4.8
-
cinnamate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda
17.3
-
4-coumarate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
54.1
-
caffeate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda
137.3
-
4-coumarate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda
287.4
-
ferulate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda