Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.2.1.12 extracted from

  • Gao, S.; Yu, H.N.; Xu, R.X.; Cheng, A.X.; Lou, H.X.
    Cloning and functional characterization of a 4-coumarate CoA ligase from liverwort Plagiochasma appendiculatum (2015), Phytochemistry, 111, 48-58 .
    View publication on PubMed
Search for more literature for 6.2.1.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Plagiochasma appendiculatum

Protein Variants

Protein Variants Comment Organism
A251S the mutant enzyme shows a lower binding affinity and catalytic efficiency than the wild type enzyme Plagiochasma appendiculatum
A251S/M247Y the mutant enzyme shows a lower binding affinity and catalytic efficiency than the wild type enzyme Plagiochasma appendiculatum
M247Y the catalytic efficiency of, 4-coumaric, caffeic and dihydro-4-coumaric acids are higher in the mutant than in the wild type enzyme Plagiochasma appendiculatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.155
-
 4-coumarate mutant enzyme M247Y, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.1887
-
 4-coumarate wild type enzyme, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.1929
-
 caffeate mutant enzyme M247Y, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.2089
-
 caffeate wild type enzyme, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.253
-
 dihydro-4-coumarate mutant enzyme M247Y, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.283
-
 4-coumarate mutant enzyme M247Y/A251S, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.389
-
 4-coumarate mutant enzyme A251S, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.4418
-
 dihydro-4-coumarate wild type enzyme, at pH 7.5 and 30°C Plagiochasma appendiculatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-coumarate + CoA Plagiochasma appendiculatum 4-coumarate is the most favorable substrate AMP + diphosphate + 4-coumaroyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Plagiochasma appendiculatum A0A0D4C3L0
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.008 4 mutant enzyme M247Y/A251S, with dihydro-4-coumarate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.0093
-
 mutant enzyme A251S, with dihydro-4-coumarate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.0135
-
 mutant enzyme A251S, with caffeate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.0152
-
 mutant enzyme M247Y/A251S, with cinnamate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.0161
-
 mutant enzyme M247Y/A251S, with caffeate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.0204
-
 wild type enzyme, with dihydro-4-coumarate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.0231
-
 mutant enzyme A251S, with cinnamate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.0344
-
 mutant enzyme M247Y, with dihydro-4-coumarate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.0378
-
 wild type enzyme, with cinnamate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.0409
-
 wild type enzyme, with caffeate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.0577
-
 mutant enzyme M247Y/A251S, with 4-coumarate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.0706
-
 mutant enzyme A251S, with 4-coumarate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.1079
-
 mutant enzyme M247Y, with cinnamate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.1136
-
 wild type enzyme, with 4-coumarate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.1309
-
 mutant enzyme M247Y, with caffeate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.4561
-
 mutant enzyme M247Y, with 4-coumarate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-coumarate + CoA
-
 Plagiochasma appendiculatum AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + 4-coumarate + CoA 4-coumarate is the most favorable substrate Plagiochasma appendiculatum AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + caffeate + CoA
-
 Plagiochasma appendiculatum AMP + diphosphate + caffeoyl-CoA
-
 ?
ATP + cinnamate + CoA
-
 Plagiochasma appendiculatum AMP + diphosphate + cinnamoyl-CoA
-
 ?
ATP + dihydro-4-coumarate + CoA
-
 Plagiochasma appendiculatum AMP + diphosphate + dihydro-4-coumaroyl-CoA
-
 ?
additional information no activity with ferulate, 5-hydroxyferylate, and sinapate Plagiochasma appendiculatum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50920, calculated from amino acid sequence Plagiochasma appendiculatum
? x * 80000, thioredoxin-, His- and S-tagged enzyme, SDS-PAGE Plagiochasma appendiculatum

Synonyms

Synonyms Comment Organism
4-coumarate CoA ligase
-
 Plagiochasma appendiculatum
4-coumarate: coenzyme A ligase
-
 Plagiochasma appendiculatum
4CL1
-
 Plagiochasma appendiculatum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.084
-
 caffeate wild type enzyme, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.084
-
 dihydro-4-coumarate wild type enzyme, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.098
-
 dihydro-4-coumarate mutant enzyme M247Y, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.123
-
 4-coumarate mutant enzyme M247Y/A251S, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.216
-
 4-coumarate wild type enzyme, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.217
-
 caffeate mutant enzyme M247Y, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.221
-
 4-coumarate mutant enzyme A251S, at pH 7.5 and 30°C Plagiochasma appendiculatum
0.782
-
 4-coumarate mutant enzyme M247Y, at pH 7.5 and 30°C Plagiochasma appendiculatum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Plagiochasma appendiculatum

pI Value

Organism Comment pI Value Maximum pI Value
Plagiochasma appendiculatum calculated from amino acid sequence
-
 9.7

Expression

Organism Comment Expression
Plagiochasma appendiculatum enzyme gene transcription is induced when Plagiochasma appendiculatum thallus is treated with either salicylic acid or methyl jasmonate up