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Literature summary for 6.2.1.12 extracted from
- Analysis of five rice 4-coumarate:coenzyme A ligase enzyme activity and stress response for potential roles in lignin and flavonoid biosynthesis in rice (2013), Biochem. Biophys. Res. Commun., 430, 1151-1156.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isozyme Os4Cl1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21 | Oryza sativa |
| isozyme Os4Cl2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21 | Oryza sativa |
| isozyme Os4Cl3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21 | Oryza sativa |
| isozyme Os4Cl4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21 | Oryza sativa |
| isozyme Os4Cl5, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21 | Oryza sativa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.079 | - |
ferulate | pH not specified in the publication, 30°C, isozyme Os4Cl1 | Oryza sativa |  |
| 0.103 | - |
4-coumarate | pH not specified in the publication, 30°C, isozyme Os4Cl1 | Oryza sativa | |
| 0.136 | - |
4-coumarate | pH not specified in the publication, 30°C, isozyme Os4Cl1 | Oryza sativa | |
| 0.143 | - |
4-coumarate | pH not specified in the publication, 30°C, isozyme Os4Cl1 | Oryza sativa | |
| 0.154 | - |
caffeate | pH not specified in the publication, 30°C, isozyme Os4Cl1 | Oryza sativa | |
| 0.18 | - |
ferulate | pH not specified in the publication, 30°C, isozyme Os4Cl1 | Oryza sativa | |
| 0.212 | - |
4-coumarate | pH not specified in the publication, 30°C, isozyme Os4Cl1 | Oryza sativa | |
| 0.234 | - |
caffeate | pH not specified in the publication, 30°C, isozyme Os4Cl1 | Oryza sativa | |
| 0.262 | - |
caffeate | pH not specified in the publication, 30°C, isozyme Os4Cl1 | Oryza sativa | |
| 0.282 | - |
4-coumarate | pH not specified in the publication, 30°C, isozyme Os4Cl3 | Oryza sativa | |
| 0.282 | - |
caffeate | pH not specified in the publication, 30°C, isozyme Os4Cl3 | Oryza sativa | |
| 0.309 | - |
ferulate | pH not specified in the publication, 30°C, isozyme Os4Cl1 | Oryza sativa | |
| 0.38 | - |
ferulate | pH not specified in the publication, 30°C, isozyme Os4Cl3 | Oryza sativa | |
| 0.384 | - |
ferulate | pH not specified in the publication, 30°C, isozyme Os4Cl1 | Oryza sativa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 4-coumarate + CoA | Oryza sativa | - |
AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
| ATP + ferulate + CoA | Oryza sativa | - |
AMP + diphosphate + feruloyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | P17814 | Os4Cl1; ssp. japonica, isozyme Os4Cl1 | - |
| Oryza sativa | Q42982 | Os4Cl2; ssp. japonica, isozyme Os4Cl2 | - |
| Oryza sativa | Q67W82 | Os4Cl4; ssp. japonica, isozyme Os4Cl4 | - |
| Oryza sativa | Q6ETN3 | Os4Cl3; ssp. japonica, isozyme Os4Cl3 | - |
| Oryza sativa | Q6ZAC1 | Os4Cl5; ssp. japonica, isozyme Os4Cl5 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged enzyme from Escherichia coli strain BL21 by affinity chromatography and removal of the GST-tag | Oryza sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| anther | isozyme Os4CL2 is specifically expressed in the anther | Oryza sativa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 4-coumarate + CoA | - |
Oryza sativa | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
| ATP + 4-coumarate + CoA | best substrate | Oryza sativa | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
| ATP + caffeate + CoA | low activity | Oryza sativa | AMP + diphosphate + caffeoyl-CoA | - |
? | |
| ATP + ferulate + CoA | - |
Oryza sativa | AMP + diphosphate + feruloyl-CoA | - |
? | |
| additional information | no activity with sinapate | Oryza sativa | ? | - |
? | |
| additional information | no activity with sinapate and caffeate | Oryza sativa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4-Coumarate:coenzyme A ligase | - |
Oryza sativa |
| 4CL | - |
Oryza sativa |
| Os4Cl1 | - |
Oryza sativa |
| Os4Cl2 | - |
Oryza sativa |
| Os4Cl3 | - |
Oryza sativa |
| Os4Cl4 | - |
Oryza sativa |
| Os4Cl5 | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Oryza sativa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Oryza sativa | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Oryza sativa | isozyme Os4CL1 is downregulated by UV radiation when dark-adapted rice plants are illuminated with UV-containing white light | down |
| Oryza sativa | isozyme Os4CL3 is downregulated by UV radiation when dark-adapted rice plants are illuminated with UV-containing white light | down |
| Oryza sativa | isozyme Os4CL4 is downregulated by UV radiation when dark-adapted rice plants are illuminated with UV-containing white light | down |
| Oryza sativa | isozyme Os4CL5 is downregulated by UV radiation when dark-adapted rice plants are illuminated with UV-containing white light | down |
| Oryza sativa | Os4CL2 is strongly activated by UV irradiation, the isozyme is induced by up to 16fold at 12 h after the onset of irradiation, and expression remains high at 24 h after treatment | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the existence of a valine residue at the substrate-binding pocket may mainly affect rice enzyme activity toward sinapic acid. The amino acid sequence similarities within the Os4CL gene family share a percentage of identity between 56% and 84%, positions of the four introns in these genes are conserved, but the introns differ in length and sequence, sequence comparisons of rice isozymes, overview | Oryza sativa |
| additional information | the existence of a valine residue at the substrate-binding pocket may mainly affect the rice enzyme activity toward sinapic acid. The amino acid sequence similarities within the Os4CL gene family share a percentage of identity between 56% and 84%, positions of the four introns in these genes are conserved, but the introns differ in length and sequence, sequence comparisons of rice isozymes, overview | Oryza sativa |
| physiological function | the isozyme catalyzes the conversion of hydroxycinnamates into corresponding CoA esters for biosynthesis of flavonoids | Oryza sativa |
| physiological function | the isozyme catalyzes the conversion of hydroxycinnamates into corresponding CoA esters for biosynthesis of flavonoids. Isozyme Os4Cl shows potential involvement in flavonoid formation | Oryza sativa |
| physiological function | the isozyme catalyzes the conversion of hydroxycinnamates into corresponding CoA esters for biosynthesis of lignin | Oryza sativa |
| physiological function | the isozyme catalyzes the conversion of hydroxycinnamates into corresponding CoA esters for biosynthesis of lignin, 4-coumaric acid and ferulic acid are the two main substrates of the enzyme for monolignol biosynthesis in rice | Oryza sativa |
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