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Literature summary for 6.2.1.12 extracted from

  • Yun, M.S.; Chen, W.; Deng, F.; Yogo, Y.
    Propanil and swep inhibit 4-coumarate:CoA ligase activity in vitro (2007), Pest Manag. Sci., 63, 815-820.
    View publication on PubMed

Application

Application Comment Organism
drug development the enzyme is a target for developing effective plant growth inhibitors, overview Nicotiana tabacum

Cloned(Commentary)

Cloned (Comment) Organism
gene 4CL, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM105 Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
additional information in vitro inhibitor screening using existing herbicides, screening assay optimization, overview Nicotiana tabacum
propanil a herbicide, 71.8% inhibition at 0.1 mM, uncompetitive inhibition towards 4-coumaric acid Nicotiana tabacum
swep a herbicide, 97.1% inhibition at 0.1 mM, uncompetitive inhibition towards 4-coumaric acid Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics in presence of inhibitors Nicotiana tabacum
0.246
-
4-coumaric acid
-
Nicotiana tabacum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-coumarate + CoA Nicotiana tabacum the enzyme is involved in the phenylpropanoid pathway AMP + diphosphate + 4-coumaroyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
gene 4CL
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain JM105 by a multistep process involving anion exchange chromatography and gel filtration Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-coumarate + CoA
-
Nicotiana tabacum AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + 4-coumarate + CoA the enzyme is involved in the phenylpropanoid pathway Nicotiana tabacum AMP + diphosphate + 4-coumaroyl-CoA
-
?

Synonyms

Synonyms Comment Organism
4-coumarate:CoA ligase
-
Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
ATP
-
Nicotiana tabacum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.006
-
-
Nicotiana tabacum swep
0.0396
-
-
Nicotiana tabacum propanil