Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as His-tag fusion protein | Glycine max |
Protein Variants | Comment | Organism |
---|---|---|
E340A | 4CL1-mutant, activity almost completely abolished | Glycine max |
G333A | 4CL1-mutant, activity almost completely abolished | Glycine max |
G337A | 4CL1-mutant, activity almost completely abolished | Glycine max |
G342A | 4CL1-mutant, activity almost completely abolished | Glycine max |
I346A | 4CL1-mutant, very low activity with all substrates | Glycine max |
L344A | 4CL1-mutant, activity almost completely abolished | Glycine max |
M338A | 4CL1-mutant, activity almost completely abolished | Glycine max |
M348A | 4CL1-mutant, activity almost completely abolished | Glycine max |
additional information | construction of hybrid enzymes using different portions of isoforms 4CL1-4CL3, some of the constructed proteins were found to be inactive | Glycine max |
P285A | 4CL1-mutant, very low activity with all substrates | Glycine max |
P286A | 4CL1-mutant, minor changes in activity | Glycine max |
P343A | 4CL1-mutant, very low activity with all substrates | Glycine max |
Q334A | 4CL1-mutant, very low activity with all substrates | Glycine max |
T331A | 4CL1-mutant, minor changes in activity | Glycine max |
T339A | 4CL1-mutant, activity almost completely abolished | Glycine max |
V284A | 4CL1-mutant, minor changes in activity | Glycine max |
Y336A | 4CL1-mutant, activity almost completely abolished | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
ferulate | 4CL1 | Glycine max | |
0.005 | - |
sinapate | 4CL1 | Glycine max | |
0.009 | - |
4-coumarate | 4CL3 | Glycine max | |
0.013 | - |
caffeate | 4CL2 | Glycine max | |
0.042 | - |
4-coumarate | 4CL2 | Glycine max | |
0.049 | - |
3,4-dimethoxycinnamate | 4CL1 | Glycine max | |
0.05 | - |
4-coumarate | 4CL1 | Glycine max | |
0.05 | - |
caffeate | 4CL3 | Glycine max | |
0.081 | - |
caffeate | 4CL1 | Glycine max | |
0.14 | - |
ferulate | 4CL2 | Glycine max | |
1.1 | - |
cinnamate | 4CL3 | Glycine max | |
1.7 | - |
cinnamate | 4CL2 | Glycine max | |
3.1 | - |
ferulate | 4CL3 | Glycine max | |
4.4 | - |
cinnamate | 4CL1 | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | Glycine max | plant phenylpropanoid metabolism | AMP + diphosphate + 4-coumaroyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
isoforms 4CL1-4CL3 | - |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3,4-dimethoxycinnamate + CoA | substrate for 4CL1 but not for 4CL2 and 4CL3 | Glycine max | AMP + diphosphate + 3,4-dimethoxycinnamoyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | - |
Glycine max | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | plant phenylpropanoid metabolism | Glycine max | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + caffeate + CoA | - |
Glycine max | AMP + diphosphate + caffeoyl-CoA | - |
? | |
ATP + cinnamate + CoA | - |
Glycine max | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
ATP + ferulate + CoA | substrate for 4CL1 and 4CL2, very poor substrate for 4CL3 | Glycine max | AMP + diphosphate + feruloyl-CoA | - |
? | |
ATP + sinapate + CoA | substrate for 4CL1 but not for 4CL2 and 4CL3 | Glycine max | AMP + diphosphate + sinapoyl-CoA | - |
? |