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Literature summary for 6.2.1.12 extracted from

  • Kutsuki, H.; Shimada, M.; Higuchi, T.
    Distribution and roles of p-hydroxycinnamate:CoA ligase in lignin biosynthesis (1982), Phytochemistry, 21, 267-271.
No PubMed abstract available
Search for more literature for 6.2.1.12

Inhibitors

Inhibitors Comment Organism Structure
Zn2+
-
 Erythrina crista-galli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 sinapoate
-
 Erythrina crista-galli
0.0021
-
 ferulate
-
 Erythrina crista-galli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 97% of the activation relative to Mg2+ Erythrina crista-galli
Mg2+ required Erythrina crista-galli
Mn2+ 85% of the activation relative to Mg2+ Erythrina crista-galli
Ni2+ 81% of the activation relative to Mg2+ Erythrina crista-galli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Erythrina crista-galli

Organism

Organism UniProt Comment Textmining
Erythrina crista-galli
-
-
-
Metasequoia glyptostroboides
-
-
-
Paulownia tomentosa
-
-
-
Phyllostachys bambusoides
-
-
-
Platycladus orientalis
-
-
-
Populus x canadensis
-
-
-
Robinia pseudoacacia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Erythrina crista-galli

Source Tissue

Source Tissue Comment Organism Textmining
phloem
-
 Erythrina crista-galli
-
xylem
-
 Populus x canadensis
-
xylem
-
 Erythrina crista-galli
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Erythrina crista-galli

Storage Stability

Storage Stability Organism
-20°C, 10% or 30% glycerol, or 10% ethylene glycol, stable for 10 days Erythrina crista-galli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3,4-dimethoxycinnamate + CoA 84% of the activity relative to ferulate Erythrina crista-galli AMP + diphosphate + 3,4-dimethoxycinnamoyl-CoA
-
 ?
ATP + 4-coumarate + CoA
-
 Populus x canadensis AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + 4-coumarate + CoA
-
 Platycladus orientalis AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + 4-coumarate + CoA
-
 Metasequoia glyptostroboides AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + 4-coumarate + CoA
-
 Robinia pseudoacacia AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + 4-coumarate + CoA
-
 Paulownia tomentosa AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + 4-coumarate + CoA
-
 Phyllostachys bambusoides AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + 4-coumarate + CoA 140% of the activity relative to ferulate Erythrina crista-galli AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + 4-methoxycinnamate + CoA 68% of the activity relative to ferulate Erythrina crista-galli AMP + diphosphate + 4-methoxycinnamoyl-CoA
-
 ?
ATP + 5-hydroxyferulate + CoA
-
 Populus x canadensis AMP + diphosphate + 5-hydroxyferuloyl-CoA
-
 ?
ATP + 5-hydroxyferulate + CoA 17% of the activity relative to ferulate Erythrina crista-galli AMP + diphosphate + 5-hydroxyferuloyl-CoA
-
 ?
ATP + caffeate + CoA
-
 Populus x canadensis AMP + diphosphate + caffeoyl-CoA
-
 ?
ATP + caffeate + CoA 48% of the activity relative to ferulate Erythrina crista-galli AMP + diphosphate + caffeoyl-CoA
-
 ?
ATP + ferulate + CoA
-
 Populus x canadensis AMP + diphosphate + feruloyl-CoA
-
 ?
ATP + ferulate + CoA
-
 Erythrina crista-galli AMP + diphosphate + feruloyl-CoA
-
 ?
ATP + isoferulate + CoA as effective as ferulate Erythrina crista-galli AMP + diphosphate + isoferuloyl-CoA
-
 ?
ATP + sinapoate + CoA
-
 Populus x canadensis AMP + diphosphate + sinapoyl-CoA
-
 ?
ATP + sinapoate + CoA
-
 Robinia pseudoacacia AMP + diphosphate + sinapoyl-CoA
-
 ?
ATP + sinapoate + CoA
-
 Phyllostachys bambusoides AMP + diphosphate + sinapoyl-CoA
-
 ?
ATP + sinapoate + CoA 48% of the activity relative to ferulate Erythrina crista-galli AMP + diphosphate + sinapoyl-CoA
-
 ?
additional information most of angiosperm and gymnosperm enzymes are active with ferulate but not with sinapoate (?). The enzymes of Erythrina crista-galli, Robinia pseudoacacia and bamboo show considerable activity with sinapoate Populus x canadensis ?
-
 ?
additional information most of angiosperm and gymnosperm enzymes are active with ferulate but not with sinapoate (?). The enzymes of Erythrina crista-galli, Robinia pseudoacacia and bamboo show considerable activity with sinapoate Platycladus orientalis ?
-
 ?
additional information most of angiosperm and gymnosperm enzymes are active with ferulate but not with sinapoate (?). The enzymes of Erythrina crista-galli, Robinia pseudoacacia and bamboo show considerable activity with sinapoate Metasequoia glyptostroboides ?
-
 ?
additional information most of angiosperm and gymnosperm enzymes are active with ferulate but not with sinapoate (?). The enzymes of Erythrina crista-galli, Robinia pseudoacacia and bamboo show considerable activity with sinapoate Erythrina crista-galli ?
-
 ?
additional information most of angiosperm and gymnosperm enzymes are active with ferulate but not with sinapoate (?). The enzymes of Erythrina crista-galli, Robinia pseudoacacia and bamboo show considerable activity with sinapoate Robinia pseudoacacia ?
-
 ?
additional information most of angiosperm and gymnosperm enzymes are active with ferulate but not with sinapoate (?). The enzymes of Erythrina crista-galli, Robinia pseudoacacia and bamboo show considerable activity with sinapoate Paulownia tomentosa ?
-
 ?
additional information most of angiosperm and gymnosperm enzymes are active with ferulate but not with sinapoate (?). The enzymes of Erythrina crista-galli, Robinia pseudoacacia and bamboo show considerable activity with sinapoate Phyllostachys bambusoides ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.6
-
 Erythrina crista-galli