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Literature summary for 6.2.1.12 extracted from
- 4-Coumarate:CoA ligase from cell suspension cultures of Petroselinum hortense Hoffm. (1977), Arch. Biochem. Biophys., 184, 237-248.
General Stability
| General Stability | Organism |
|---|---|
| complete stabilization in presence of 30% glycerol or 30% ethylene glycol | Petroselinum crispum |
| sucrose stabilizes | Petroselinum crispum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | - |
Petroselinum crispum |  |
| 4-coumarate | - |
Petroselinum crispum | |
| CoA | - |
Petroselinum crispum | |
| iodoacetamide | - |
Petroselinum crispum | |
| NEM | - |
Petroselinum crispum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
caffeate | 2-coumarate | Petroselinum crispum | |
| 0.02 | - |
ferulate | caffeate | Petroselinum crispum | |
| 0.026 | - |
isoferulate | - |
Petroselinum crispum | |
| 0.028 | - |
3-coumarate | - |
Petroselinum crispum | |
| 0.033 | - |
ferulate | - |
Petroselinum crispum | |
| 0.28 | - |
cinnamate | - |
Petroselinum crispum | |
| 0.34 | - |
5-hydroxyferulate | - |
Petroselinum crispum | |
| 0.5 | - |
4-methoxyferulate | - |
Petroselinum crispum | |
| 0.55 | - |
3,4-dimethoxycinnamate | - |
Petroselinum crispum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 33% of the activation compared to Mg2+ | Petroselinum crispum | |
| Mg2+ | required in a molar ratio of 1:1 with ATP | Petroselinum crispum | |
| Mn2+ | 97% of the activation compared to Mg2+ | Petroselinum crispum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
gel filtration | Petroselinum crispum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Petroselinum crispum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Petroselinum crispum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Petroselinum crispum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Petroselinum crispum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| complete loss of activity in crude enzyme extract after 4 days at 4-6°C. 0.92 M sucrose can reduce the loss of activity to 20%. Complete stabilization in presence of 30% glycerol or 30% ethylene glycol | Petroselinum crispum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-coumarate + CoA | - |
Petroselinum crispum | AMP + diphosphate + 2-coumaroyl-CoA | - |
? | |
| ATP + 3,4,5-trimethoxycinnamate + CoA | no activity | Petroselinum crispum | AMP + diphosphate + 3,4,5-trimethoxycinnamoyl-CoA | - |
? | |
| ATP + 3,4-dimethoxycinnamate + CoA | - |
Petroselinum crispum | AMP + diphosphate + 3,4-dimethoxycinnamoyl-CoA | - |
? | |
| ATP + 3-coumarate + CoA | - |
Petroselinum crispum | AMP + diphosphate + 3-coumaroyl-CoA | - |
? | |
| ATP + 4-coumarate + CoA | - |
Petroselinum crispum | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
| ATP + 4-methoxycinnamate + CoA | - |
Petroselinum crispum | AMP + diphosphate + 4-methoxycinnamoyl-CoA | - |
? | |
| ATP + 4-methoxyferulate + CoA | - |
Petroselinum crispum | AMP + diphosphate + 4-methoxyferuloyl-CoA | - |
? | |
| ATP + 5-hydroxyferulate + CoA | - |
Petroselinum crispum | AMP + diphosphate + 5-hydroxyferuloyl-CoA | - |
? | |
| ATP + caffeate + CoA | - |
Petroselinum crispum | AMP + diphosphate + caffeoyl-CoA | - |
? | |
| ATP + cinnamate + CoA | - |
Petroselinum crispum | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
| ATP + ferulate + CoA | - |
Petroselinum crispum | AMP + diphosphate + feruloyl-CoA | - |
? | |
| ATP + isoferulate + CoA | - |
Petroselinum crispum | AMP + diphosphate + isoferuloyl-CoA | - |
? | |
| ATP + sinapoate + CoA | no activity | Petroselinum crispum | AMP + diphosphate + sinapoyl-CoA | - |
? | |
| additional information | absolute requirement for ATP | Petroselinum crispum | ? | - |
? | |
| additional information | ATP-diphosphate exchange reaction is activated by cinnamate, dihydro-4-coumarate, caprylate and malonate | Petroselinum crispum | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Petroselinum crispum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | ATP-diphosphate exchange | Petroselinum crispum |
| 7.8 | 8 | formation of 4-coumaroyl-CoA | Petroselinum crispum |
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Release 2023.1 (January 2023)
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