Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Dunaliella tertiolecta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.59 | - |
potassium acetate | at pH 8.0 and 37°C | Dunaliella tertiolecta | |
4.66 | - |
Sodium acetate | at pH 8.0 and 37°C | Dunaliella tertiolecta |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Dunaliella tertiolecta | 5886 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | Dunaliella tertiolecta | - |
AMP + diphosphate + acetyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dunaliella tertiolecta | A0A144QFN1 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap affinity column chromatography | Dunaliella tertiolecta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.885 | - |
crude extract, at pH 8.0 and 37°C | Dunaliella tertiolecta |
52.873 | - |
after 59.7fold purification, at pH 8.0 and 37°C | Dunaliella tertiolecta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | - |
Dunaliella tertiolecta | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + potassium acetate + CoA | - |
Dunaliella tertiolecta | AMP + acetyl-CoA + potassium diphosphate | - |
? | |
ATP + sodium acetate + CoA | - |
Dunaliella tertiolecta | AMP + acetyl-CoA + sodium diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 100000, thiordeoxin-tagged enzyme, SDS-PAGE | Dunaliella tertiolecta |
? | x * 79720, calculated from amino acid sequence | Dunaliella tertiolecta |
Synonyms | Comment | Organism |
---|---|---|
ACS | - |
Dunaliella tertiolecta |
AMP-forming acetyl-CoA synthetase | - |
Dunaliella tertiolecta |
AMP-forming ACS | - |
Dunaliella tertiolecta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Dunaliella tertiolecta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Dunaliella tertiolecta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Dunaliella tertiolecta |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Dunaliella tertiolecta | isoelectric focusing | - |
6.7 |