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Literature summary for 6.2.1.1 extracted from

  • Liang, M.H.; Qv, X.Y.; Jin, H.H.; Jiang, J.G.
    Characterization and expression of AMP-forming acetyl-CoA synthetase from Dunaliella tertiolecta and its response to nitrogen starvation stress (2016), Sci. Rep., 6, 23445 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Dunaliella tertiolecta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.59
-
potassium acetate at pH 8.0 and 37°C Dunaliella tertiolecta
4.66
-
Sodium acetate at pH 8.0 and 37°C Dunaliella tertiolecta

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
Dunaliella tertiolecta 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetate + CoA Dunaliella tertiolecta
-
AMP + diphosphate + acetyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Dunaliella tertiolecta A0A144QFN1
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap affinity column chromatography Dunaliella tertiolecta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.885
-
crude extract, at pH 8.0 and 37°C Dunaliella tertiolecta
52.873
-
after 59.7fold purification, at pH 8.0 and 37°C Dunaliella tertiolecta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate + CoA
-
Dunaliella tertiolecta AMP + diphosphate + acetyl-CoA
-
?
ATP + potassium acetate + CoA
-
Dunaliella tertiolecta AMP + acetyl-CoA + potassium diphosphate
-
?
ATP + sodium acetate + CoA
-
Dunaliella tertiolecta AMP + acetyl-CoA + sodium diphosphate
-
?

Subunits

Subunits Comment Organism
? x * 100000, thiordeoxin-tagged enzyme, SDS-PAGE Dunaliella tertiolecta
? x * 79720, calculated from amino acid sequence Dunaliella tertiolecta

Synonyms

Synonyms Comment Organism
ACS
-
Dunaliella tertiolecta
AMP-forming acetyl-CoA synthetase
-
Dunaliella tertiolecta
AMP-forming ACS
-
Dunaliella tertiolecta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Dunaliella tertiolecta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Dunaliella tertiolecta

Cofactor

Cofactor Comment Organism Structure
ATP
-
Dunaliella tertiolecta

pI Value

Organism Comment pI Value Maximum pI Value
Dunaliella tertiolecta isoelectric focusing
-
6.7