Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41(DE3) cells | Streptomyces lividans |
Protein Variants | Comment | Organism |
---|---|---|
S608T | when mutant enzyme S608T is acetylated, it loses about 80% activity compared to the wild type | Streptomyces lividans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | when the enzyme is acetylated, it loses about 20% activity compared to the non-acetylated control | Streptomyces lividans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | Streptomyces lividans | - |
AMP + diphosphate + acetyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lividans | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
acetylation | - |
Streptomyces lividans |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography, and Superdex 200 gel filtration | Streptomyces lividans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | - |
Streptomyces lividans | AMP + diphosphate + acetyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72000, His6-tagged enzyme, SDS-PAGE | Streptomyces lividans |
Synonyms | Comment | Organism |
---|---|---|
Acetyl-CoA synthetase | - |
Streptomyces lividans |
ACS | - |
Streptomyces lividans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Streptomyces lividans |