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Literature summary for 6.2.1.1 extracted from

  • Tajima, K.; Han, X.; Hashimoto, Y.; Satoh, Y.; Satoh, T.; Taguchi, S.
    Invitro synthesis of polyhydroxyalkanoates using thermostable acetyl-CoA synthetase, CoA transferase, and PHA synthase from thermotorelant bacteria (2016), J. Biosci. Bioeng., 122, 660-665 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Pelotomaculum thermopropionicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetate + CoA Pelotomaculum thermopropionicum
-
AMP + diphosphate + acetyl-CoA
-
?
ATP + acetate + CoA Pelotomaculum thermopropionicum JCM10971
-
AMP + diphosphate + acetyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Pelotomaculum thermopropionicum
-
-
-
Pelotomaculum thermopropionicum JCM10971
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Pelotomaculum thermopropionicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate + CoA
-
Pelotomaculum thermopropionicum AMP + diphosphate + acetyl-CoA
-
?
ATP + acetate + CoA
-
Pelotomaculum thermopropionicum JCM10971 AMP + diphosphate + acetyl-CoA
-
?

Synonyms

Synonyms Comment Organism
acetyl CoA synthetase
-
Pelotomaculum thermopropionicum
ACS
-
Pelotomaculum thermopropionicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Pelotomaculum thermopropionicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
72
-
the enzyme shows long-term stability at 45°C for at least 72 h Pelotomaculum thermopropionicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Pelotomaculum thermopropionicum

Cofactor

Cofactor Comment Organism Structure
ATP
-
Pelotomaculum thermopropionicum