Cloned (Comment) | Organism |
---|---|
epxression in Escherichia coli | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
D-Ala | cosubstrate D-Ala, binding of N-terminal Ala residue. pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
3 | - |
D-2-hydroxybutanoate | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
8.3 | - |
D-2-hydroxyvalerate | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
11.4 | - |
D-lactate | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
34 | - |
D-Ala | cosubstrate D-Ala, binding of C-terminal Ala residue. pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, SDS-PAGE | Enterococcus faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
V583, isoform VanB | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ala + D-2-hydroxybutanoate + ATP | - |
Enterococcus faecalis | D-Ala-D-2-hydroxybutanoate + ADP + phosphate | - |
? | |
D-Ala + D-2-hydroxyvalerate + ATP | - |
Enterococcus faecalis | D-Ala-D-2-hydroxyvalerate + ADP + phosphate | - |
? | |
D-Ala + D-Ala + ATP | - |
Enterococcus faecalis | D-Ala-D-Ala + ADP + phosphate | - |
? | |
D-Ala + D-lactate + ATP | - |
Enterococcus faecalis | D-Ala-D-lactate + ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | Enterococcus faecalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
D-2-hydroxybutanoate | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
0.42 | - |
D-2-hydroxyvalerate | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
0.47 | - |
D-lactate | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
4.1 | - |
D-Ala | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0414 | - |
D-lactate | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
0.05 | - |
D-2-hydroxyvalerate | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
0.084 | - |
D-2-hydroxybutanoate | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis | |
0.12 | - |
D-Ala | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecalis |